Equipment Name: Differential Centrifugal Sedimentation (DCS) or analytical centrifugation

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21 Φεβ 2014 (πριν από 3 χρόνια και 5 μήνες)

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Eq
u
ipme
n
t

N
a
me:

D
i
f
f
erential

C
e
ntr
i
fugal
S
e
dimentat
i
on (DCS)

or ana
l
y
t
i
cal
ce
n
tri
f
ug
a
t
i
on


C
a
tego
r
y
:

A
.

P
a
rticle

s
ynthe
s
is (cha
r
a
ct
e
ri
s
atio
n
)


C. Pa
r
ticle Cha
r
a
cte
r
i
s
ation

i
n

a
n
d

e
x
-
situ


I
n
stitute:

U
n
iv
e
rsi
t
y

C
o
l
l
ege D
u
blin


L
o
c
a
ti
o
n:

C
e
ntre

f
or

Bi
o
Nano

I
nter
a
ctio
n
s,

C
o
n
w
a
y

I
nstit
u
te
,
U
n
iv
e
r
s
i
t
y

C
o
l
l
e
g
e
D
u
b
l
in,

B
e
l
fie
l
d, Dub
l
i
n 4, Ire
l
and


Conta
c
t

D
e
tails of
T
e
c
h
n
o
logy

Exp
e
rt:
N
a
m
e:
Dr. Marco Mo
n
o
p
o
l
i

Ph
o
ne:

+353

1 716 2418

Fa
x
:

E
-
m
a
i
l
:
marc
o
.mo
n
o
p
o
l
i
@
c
bn
i.ucd.ie


Short t
e
ch
n
ology

descripti
o
n/O
v
e
r
v
i
e
w
:



Differential

C
entrifugal
Se
dimentat
i
on

(
DS
C
) or a
n
a
l
y
t
ical centrif
u
gation

is

s
u
it
a
ble
f
or

high

resoluti
o
n part
i
cle s
i
ze
c
h
a
racter
i
s
a
t
i
on of

v
i
rtually

any

material

from 0
.
003

m
i
c
r
o
n (
3
nm) to

50 micron (
d
epending on
t
he pa
r
t
i
cle d
e
n
s
it
y
).
Particle

size distri
b
utio
n
s a
r
e m
e
asur
e
d usi
n
g a sp
i
nning d
i
sc wi
t
h a sucr
o
se

g
radi
e
nt

to

s
e
p
arate p
a
rtic
le
s on the
ba
s
i
s of

s
i
z
e
,

and the s
y
st
e
m c
a
n
r
outin
e
ly sepa
r
ate
p
articl
e
s that

d
if
f
er in

si
z
e by

as

l
i
t
t
le as 2
-
5
%
,

includi
n
g

in
c
o
mp
l
ex

s
o
lu
t
ions

such
a
s pla
s
m
a or

c
e
l
l culture
m
ed
i
a
.

Unli
k
e mo
s
t

other pa
r
t
i
c
l
e si
z
i
ng
t
ech
n
iqu
e
s, partic
l
es

a
r
e
a
c
tually

s
ep
a
rated a
n
d
t
h
e
n mea
s
ured, so no
p
red
i
ct
i
ve

algo
r
ith
m
s

are u
s
ed.


The

results
f
rom a

DSC s
h
o
w

alm
o
st "c
h
ro
m
atog
r
aph
i
c"

t
y
pe

r
e
s
o
l
ution wh
i
ch

i
s

in dr
a
matic con
t
ra
s
t

with other
pa
r
tic
l
e si
z
in
g

te
c
hni
q
u
e
s

e.g.

light s
c
at
t
ering met
h
od
s
. The ult
r
a
-
hi
g
h resol
u
tion

m
a
kes

t
his an

i
deal method

f
o
r
ef
f
e
c
ti
v
ely res
o
l
v
ing

a
ggr
e
gates

a
nd

a
g
glom
e
rat
e
s
, and

for

o
b
serv
i
ng

re
l
ati
v
e

s
h
if
t
s

in

p
e
a
ks

a
nd

tails on

pa
r
t
i
c
l
e
distri
b
ution
c
ha
r
ts
th
at

are

not clea
r
ly
v
isible

with o
th
er
t
e
c
h
niq
u
es.



UCD have

de
veloped pro
to
c
o
l
s

for the s
t
udy

of

n
anoparticl
e
s ef
f
e
c
t
i
ve

s
i
ze

in situ

in c
o
mp
l
ex

b
iofluids, wh
i
ch

can

b
e
made

avai
l
a
b
le

thro
u
gh

Q
Na
n
o

TA.

Ap
p
licabil
i
ty of

these

pro
t
oco
l
s

to

oth
e
r

bi
o
fluids,

su
c
h

a
s

riv
e
r

w
ater

c
o
nta
i
ni
n
g
natural
o
rgan
i
c mat
t
er a
n
d nan
o
pa
r
t
i
cles

disp
e
rsed in

c
o
n
s
u
m
er
p
roduc
t
s (
e
.g.

cr
èmes) a
r
e u
n
derway.


Main

Featu
r
es (Equ
i
pm
e
nt Ca
p
abi
l
ities
)
:



Size R
a
nge

Ca
p
ability


The p
r
a
c
t
i
c
a
l

measu
r
ement

range in

a
ny

appl
i
c
a
tion

d
epends
u
pon

the de
n
sity

of

the pa
r
ticl
e
s

to

be measu
r
ed. With
very

dense
p
articl
e
s (f
o
r
e
xample,

6

ti
m
es

t
he d
e
n
s
i
t
y

of

water) t
h
e ma
x
imum
a
nd minimum

sizes
a
re
sm
aller (/
1
0
micro
n
s to

u
n
der

0
.
0
0
5 mi
c
ro
n
). Wi
t
h low den
s
ity

part
i
cles (for e
xa
mple,

from 0
.
85 g/
c
c to

1.10 g
/
cc) the

m
axi
m
um
and mini
m
um

sizes
a
re la
r
ger

(/
7
5 mi
cr
ons

t
o
/
0.02

micron). T
h
e pr
a
ctic
a
l d
yn
amic
r
an
g
e (ra
t
io of

larg
e
st
t
o smalle
s
t
sizes)
i
n a single

anal
y
sis

i
s about

70 using fi
x
ed cen
t
rifuge spe
e
d,

and up
t
o 1
00
0 usi
n
g ra
m
p
i
ng of

s
p
e
ed
d
uring
the analys
i
s. For
f
i
x
ed sp
e
ed, if

the lar
g
e
s
t

pa
r
t
i
cle
t
o

be measu
r
ed

is 3

micro
n
s,
t
hen pa
r
tic
le
s as

small
a
s

0.02
micron
c
an
n
ormally

be
m
easur
e
d in

a sing
l
e analys
i
s of

less
t
han 1

hou
r
.

F
o
r
r
amped

s
p
e
e
d
,

if the
l
arg
e
st

particle




to

be measu
r
ed is 10 m
i
cron
s
,

then
p
ar
t
icles le
s
s th
a
n 0
.
02 mi
c
ron

could
n
or
m
a
l
ly be mea
s
ur
ed in

a

single

run.



Anal
y
sis

T
im
e


Anal
y
sis

t
ime

will

depend
u
pon the ran
g
e of sizes
t
h
a
t

is being a
na
l
y
z
e
d a
n
d
t
he

density

of

t
he particles

b
e
ing
measu
r
ed. For

the

maj
o
rity

of

applicati
o
n
s
,

anal
y
sis

ti
mes
a
re in

t
h
e range

of

3
t
o 15
m
inut
e
s
f
per sample.

For
ex
t
remely
f
ine partic
l
es

(0
.
005 to

0.06
m
ic
r
o
n),

the a
n
alysis ti
m
e
ca
n re
a
ch

30 m
i
nutes
o
r mo
r
e.

CPS

has

d
evelop
e
d
techn
i
qu
e
s to

allow a
n
alys
i
s of

most

t
y
pes

of

v
ery

s
m
all partic
l
es

in

a

reason
a
ble

t
ime.

We can

m
ea
s
u
re
t
he

size
distri
b
ution of

one or m
o
re
of

your speci
f
ic s
a
m
pl
e
s to

determ
i
ne
t
he

e
x
a
c
t

analys
i
s ti
m
e

pri
o
r to

y
our

appl
i
c
a
t
ion,

in
ord
e
r to

have

a

more
a
ccura
t
e estima
t
e

of

the
v
isit duration
r
equ
i
re
m
ent
s
.


Iss
u
es

t
o
c
o
n
sid
e
r

when
d
e
s
i
g
ning TA

e
x
p
e
ri
m
ent
s
:





D
e
n
s
ity

of

y
our

p
a
rticl
e
s


the
lower l
i
mit

of

separation

is dep
e
nd
e
nt

on
t
he partic
l
e de
n
sity;





Co
n
c
e
ntrati
o
n range

for
u
se

in

the me
a
s
u
r
em
e
nt
s
;




When

me
a
suring n
a
no
m
ateria
l
s

s
i
ze

a
n
d size

distr
i
b
u
tion

in comp
l
ex

media,

con
t
rol s
a
mpl
e
s

in buf
f
er or s
i
mple
s
o
luti
o
ns

s
h
o
uld al
s
o be

p
erfor
m
ed.



T
y
p
ical
S
a
m
ples & I
m
a
g
es:




D
C
S equ
i
p
m
ent,

and

ex
a
mple

of det
e
rminati
o
n

of the

ef
f
e
c
ti
v
e

size

of

n
an
o
pa
r
tic
l
es

in

t
he

a
b
sen
c
e

(b
a
re)

a
n
d
pr
e
s
e
n
ce

(
coron
a
)

of

h
u
m
an

pl
a
sma.

T
he

co
r
ona

i
s very

simil
a
r

i
n

situ
and

fol
l
owi
n
g

w
ashi
n
g

to

re
m
ove

t
he

lo
o
s
e
ly
bound

prote
in
s.




Key ref
e
re
n
ces:





Mono
p
oli
M
P,

Walczyk

D, Cam
p
bell
A
,

El
i
a G,

L
y
n
c
h

I
,

Bombelli

FB, Da
w
s
o
n
K
A.

Ph
y
si
c
al
-
chemi
c
al a
s
p
e
cts of
protein

coro
n
a:

releva
n
ce

t
o in

v
i
tro and in

v
i
vo

biologi
c
al i
m
p
a
cts of

nano
p
artic
le
s.

J Am

Ch
e
m
So
c
.

201
1
,

133
,
2
5
2
5
-
2
5
34.





Wa
l
c
z
y
k D,
B
ombelli F
B
,

Mono
p
oli
M
P,

L
y
n
c
h
I
, Da
w
son

K
A
.

Wh
a
t

the c
e
ll "sees"

in

bionan
o
scie
n
c
e
. J Am

Chem
So
c.
2
01
0
,

132,

5
76
1
-
5
7
68.




Any
f
urth
e
r I
n
for
m
atio
n
:


Al
t
hou
g
h

not

s
p
e
cific

a
s

a

UCD

i
n
s
tal
l
ation,

p
ro
p
osals

u
s
i
ng

D
S
C

t
o

c
h
a
racter
i
se

nan
o
m
ater
i
al

disp
er
sio
n
s

in

situ

in
c
o
mp
l
ex

flui
d
s

c
a
n

also

pe
rform

me
a
su
r
ements

u
sing

dynam
i
c

lig
h
t

scat
t
ering

(
DLS)

and

Na
noSight

f
o
r

comp
a
rison




of

the si
z
e
s
a
nd s
i
ze

d
i
stribu
t
ions

by

t
h
e
se

m
eth
o
d
s
.




If required,
a
ccess

to

h
u
m
an

pl
a
sma acquir
e
d

u
sing

f
rom

the Dubl
i
n

Blood

B
a
nk

c
a
n also

be provid
e
d.

Th
i
s

shou
l
d
be

ind
i
c
a
ted

in

the

appl
i
cation

form,

a
l
ong

with

t
h
e

volume

req
u
e
s
ted

(t
y
pica
l
ly

15

mls

ar
e

s
u
f
f
ic
i
ent

for

p
rotein
c
o
r
ona studi
e
s
)
.

If U
s
ers are

b
rin
g
i
n
g

their

o
wn

hu
m
an

biolog
i
c
a
l s
a
mp
l
e
s
,

full

ethical

a
p
pr
o
val

must

be

in

pla
c
e

f
o
r
this,

and
UC
D re
q
ui
r
es

copies in
a
dva
n
ce

of

any

s
a
mples

b
eing

bro
u
ght to

U
C
D
.




The

D
CS

ins
t
allation

c
a
n

be

u
s
e
d

in

conju
n
ction

w
i
th

the

EM

i
n
stallation

for

c
h
a
racter
i
s
a
t
i
on

of

nan
o
m
ateria
l
s

in
c
o
mp
l
ex

me
d
ia.

A
n e
x
ample of

s
u
ch

a
c
omp
a
rison is

s
h
o
wn in

Wa
l
c
z
y
k et

al.,

JAC
S
,

2010,

1
32,

576
1
-
5
76
8.