Genetic Engineering and Genes (GE) (PDF , 24kb)

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11 Δεκ 2012 (πριν από 4 χρόνια και 6 μήνες)

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Yr3op1
1
-
12
14
(10 credits)
-
Module No.0330101
GENETIC ENGINEERING AND GENES (GE)
Module Coordinator:Prof A J Wilkinson
Autumn
This module will begin with a brief introduction to genes and genomes.There will
follow an exploration of
factors regulating gene expression in bacteria and a discussion of how knowledge of gene control can be
exploited to express foreign (human) proteins in genetically modified micro
-
organisms.The course will
continue with a descript
ion of the molecular events associated with the remarkable process of protein
biosynthesis with an emphasis on the insights emerging from the structure of the ribosome.We will go on to
explore techniques for isolating and precisely manipulating genes.The
re will then follow a discussion of the
structure of genomes and the emerging field of genomics.Finally,we will illustrate how genetic engineering
is making penetrating breakthroughs in our understanding of protein function and in the treatment and
diag
nosis of disease.
Topics:
Control of Gene Expression
:
We will discuss how transcription is controlled in bacterial cells introducing the concept of
regulatory ‘consensus’ sequences and describing the structure and function of bacterial
RNA polymer
ase.We will go on to describe the interplay of proteins and DNA sequences
in the regulation of transcription in the
lac
operon,and genetic decision making in
bacteriophage lambda.
AJW
7
lectures
Protein Synthesis
:
The amino acid residues along a pro
tein chain are linked by peptide bonds.These bonds
are thermodynamically unstable so energy is required for their formation.The complex
cellular machinery of protein biosynthesis is described covering transcription of DNA into
RNA and the role of trans
fer RNAs in translating the genetic code into messenger RNA at
the ribosome.
KSW
5
lectures
Genomes and Genetic Engineering
:
After a consideration of DNA replication,we will explore how our knowledge of gene
structure and function can be exploited th
rough genetic engineering.Methods of gene
cloning and expression will be covered as will methods for sequencing DNA.At the heart of
the discussion will be the techniques of DNA hybridisation and polymerase chain reaction
(PCR).We will finish the course
with further consideration of results emerging from
genome sequencing projects together with a case study illustrating the impact of genetic
engineering.
AJW
6
lectures
Workshop:
1 x 3h
AJW/KSW
Design of oligon
ucleotides for Genetic
Engineering
Tutorials:
1
KSW/AJW
Gene Control & Protein Biosynthesis
/General Revision
Prerequisites:
Core Modules 1
-
4
A
ssessment:
1
1
4
h written paper (80%) (Week 1,Term 8) plus in
-
course assessed exercise associated
with workshop (20%).