Syllabus for Entrance Test in M.Sc. Biotechnology

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12 Φεβ 2013 (πριν από 4 χρόνια και 2 μήνες)

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Syllabus for Entrance Test in M.Sc. Biotechnology

History, development and scope of microbiology; general characteristics of micro-organisms;
structural and reproductive diversity, classification and economic importance of bacteria; general
characteristics of actinomycetes, cyanobacteria, mycoplasma, fungi and algae. Biochemical and
immunological characterization of microbes; general account of Rickettsiae, viruses, Chlamydiae;
microbial associations, microbial ecosystem, microbial growth; microbiology of air, water and soil.
Biological N
2
-fixation. Genetics and regulation of N
2
– fixation, hydrogen metabolism, cyanophages.
Industrial microbiology: Use of microbes in fermentation, production of antibiotics, enzymes,
organic acids, alcohol and vitamins; biofertilizer, lignocellulose bioconversion; mycorrhizae;
biopesticides, integrated pest management, quarantine; sewage treatment, bio-remediation biogas
generation; microbes from extreme environment, single cell protein, microbial toxins and human
health.
General introduction, scope & importance of biochemistry. Water and it’s biological
significance; structure, classification and biological importance of carbohydrates, lipids, proteins,
nucleic acids, enzymes, vitamins, steroids, pigments and hormones; chemical equilibrium, biological
transport process; biosynthesis of carbohydrates in animal tissues, glycogen metabolism and genetic
defects, regulation of lactose synthesis; metabolism of lipids / nucleic acids , proteins; mechanism of
enzyme action, kinetics; oxidation of fatty acids in animal tissues, its regulation and inhibition, amino
acid catabolism, urea cycle, genetic defects, glycogenesis, glycogenolysis and gluconeogensis etc.
Ultra-structure of the generalized eukaryotic cell and cell organelles; cell division, chromosome
structure, special types of chromosomes, modern concepts of gene, linkage and crossing over,
mendelism, interaction of genes, extra chromosomal inheritance, gene mutation; DNA structure and
replication, RNA structure and types, protein biosynthesis, gene expression and regulation-operon
concept, central dogma, gene mutation, DNA damage and repair; scope and importance of genetic
engineering and biotechnology, plasmids, cosmids, vectors, gene cloning, gene transfer and
recombinant DNA technology, application in agriculture, medicine and industry, microbes as tools in
biotechnology; somatic hybridization, protoplast fusion.
Basic concepts of immunity, antigen, antibodies, immunological techniques, immune response
to tumor cells, bacterial, viral, fungal immunology, immunoprophylaxis, immunotherapy.
Chromatography, electrophoresis, centrifugation, electron microscopy , fluorescence
microscopy, phase contrast microscopy, NMR spectroscopy, spectrophotometry, colorimetry, isolation,
preservation, improvement of microbial cultures, microbial growth kinetics, stains, DNA sequencing &
fingerprinting, PCR, blotting techniques, fermentors, bioreactors, immobilization, basic concepts on
plant and animal tissue culture.
Water relation and mineral nutrition in plants; metabolism and bioenergetics- respiratory
metabolisms, photosynthesis; plant growth and development.
Concepts of biodiversity, endangered plant species, conservation, ethnobotany, taxonomic
characters and medicinal importance of angiospermic plants.
Basic concept, factors, ecosystem, energy flow, food chains, recycling of matters, Ecological
types, succession, pollution, green belt designing, dryland farming, social and agroforestry, metal
prospecting, biodiversity etc.
Theorems of probability, measurement of central tendencies and dispersion, t and X
2
tests,
basic structure and functioning of computers, application of computer in biology. basic concept of
bioinformatics.