PV92/PCR Bioinformatics Power Point Follow-up Questions (Pre-lab ...

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1 Οκτ 2013 (πριν από 4 χρόνια και 1 μήνα)

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PV92/PCR Bioinformatics

Power Point Follow
-
up Questions (Pre
-
lab evaluation)


1.

What is the purpose of this lab? What are we trying to do?

2.

What is PCR and why is it important?

3.

Label each step and d
escribe what happens during the three steps in PCR.

a.

Heat to

94°C:

b.

Cool to 60°C:

c.

Warm to 72°C:

4.

Write a short definition of the following terms as related to PCR:

a.

Template DNA:

b.

Primers:

c.

Nucleotides:

d.

Taq Polymerase:

5.

What is the source of this laboratory’s template DNA?

6.

What are the three possible results for the alle
le we are amplifying?

7.

What is the difference between and intron and an exon?