Genomics II: Functional Genomics, Proteomics, and Bioinformatics

moredwarfΒιοτεχνολογία

1 Οκτ 2013 (πριν από 4 χρόνια και 1 μήνα)

90 εμφανίσεις

Chapter 22

Genomics II: Functional Genomics, Proteomics, and Bioinformatics


Study Outline

1.

Functional genomics

a.

A microarray can identify genes that are transcribed

b.

DNA microarrays can be used to identify DNA
-
protein binding at the genome level

c.

Gene knocko
ut collections allow researchers to study gene function at the genomic level

2.

Proteomics

a.

The proteome is much larger than the genome

b.

Two
-
dimensional gel electrophoresis is used to separate a mixture of cellular proteins

c.

Mass spectrometry is used to identify

proteins

d.

Protein microarrays can be used to study protein expression and function

3.

Bioinformatics

a.

Sequence files are analyzed by computer programs

b.

The scientific community has collected sequence files and stored them in large computer
databases

c.

Different c
omputational strategies can identify functional genetic sequences

d.

Homologous genes are derived from the same ancestral gene

e.

Genetic sequences can be used to predict the structure of RNA and proteins