Image Processing Using ImageJ

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6 Νοε 2013 (πριν από 3 χρόνια και 5 μήνες)

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Image Processing Using ImageJ

Norbert Vischer

Centre for Advanced Microscopy

University of Amsterdam

Image Processing

Part 1

Image processing Image Analysis

Data Organization

From Images to Results

From Images to Results

ImageJ

Menus

Full well capacity:

15 000 electrons

Image as a matrix

Image

as a matrix (CCD)

An

image is a matrix of numbers

4 MP camera:


1 MPixel red

2 MPixel green

1 MPixel blue

4 MP color image


4 MPixel red

4 MPixel green

4 MPixel blue

Missing fields are interpolated

Color images

Color

images

File types

File

types (Save Command)

TIFF

(Tag Image File Format) :


8
-
bit 16 bit, 32bit, RGB,
uncompressed in ImageJ


JPEG

/ JFIF:


Lossy Compression, 1:25


GIF

(Graphics Interchange Format) :


8 bit Graphics, Animation, lossless


PNG

(Portable Network Graphics):


RGB Graphics, Transparency,
lossless, superior to GIF, for
screenshots


DICOM


FITS

File types

File

types

-

8 Bit

-

16 Bit

-

RGB color

-
32 Bit Floating point

-
---------------------------

-


(Hyper
-
)Stacks

-


Metadata

-


Spatial calibration

-
Density calibration



-


Only uncompressed TIFF in
ImageJ

TIFF is the most versatile format

TIFF: A File system within a file

TIFF

Format

Hyperstacks need TIFF


JPEG Compression / Decompression

JPEG

Compression

JPEG Artifacts

8 * 8 cell

JPEG Artifacts

Bit depth of image types

Bit

depth