Wu, W - Chemical and Biomolecular Engineering

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12 Φεβ 2013 (πριν από 4 χρόνια και 2 μήνες)

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Wu, W. Y., Gilles, A. R., Hsii, J., Contreras, L., Oak, S
., Perl, M. B., & Wood, D. W.,

Self
-
cleaving purification tags re
-
engi
neered for rapid Topo® cloning,”
Biotechnology Progress

(In press)
.


Fong, B. A., Wu, W.
-
Y. & Wood, D. W., “The potential role o
f self
-
cleaving
purification tags in commercial
-
scale processes,”
Trends in Biotechnology

(
Published online March 30, 2010
).


Wu, W.
-
Y., Fong, B. A., Gillies, A. R. & Wood, D. W., “Recombinant Protein
Purification by Self
-
cleaving Elastin
-
like Polypeptide
Fusion Tag,”
Current
Protocols in Protein Science
, Chapter 26: Unit 26.4.1
-
18,

(2009)
.


Gawrys, M. D., Hartman, I., Landweber, L. F. & Wood, D. W., “Use of engineered
Escherichia coli Cells to Detect Estrogenicity in Everyday Consumer Products,”
Journal of

Chemical Technology and Biotechnology
, Vol. 84, pp. 1834
-
1840,
(2009).


Hartman, I., Gillies, A. R., Arora, S., Andaya, C., Royapet, N., Welsh, W. J., Zauhar,
R J. & Wood, D. W., “Novel Screening Methods Using Shape Signatures and
Engineered Biosensors fo
r Identification of Estrogen Antagonists,”
Pharmaceutical Research
, Vol. 26 (10), pp.
2247
-
2258
, (2009).


Fong, B. A., Wu, W.
-
Y. & Wood, D. W., “Optimization of ELP
-
intein mediated
protein purification by salt substitution,”
Protein Expression and Purifica
tion
,
Vol. 66 (2), pp. 198
-
202, (2009).


Gillies, A. R., Hsii, J. F., Oak, S. & Wood, D. W., “Rapid cloning and purification of
proteins: Gateway vectors for protein purification by self
-
cleaving tags (
Editors’
Choice feature publication
),”
Biotechnology

a
nd Bioengineering
, Vol. 101 (2),
pp. 229
-
240, (2008).


Staii, C., Wood, D. W. & Scoles, G., “Ligand
-
induced Structural Changes in Maltose
Binding Proteins Measured by Atomic Force Microscopy,”
Nano Letters
, Vol. 8
(8), pp. 2503
-
2509, (2008).


Staii, C., Wo
od, D. W. & Scoles, G., “Verification of biochemical activity for
proteins nanografted on gold surfaces,”
Journal of the American Chemical
Society
, Vol. 130 (2), pp. 640
-
646, (2008).


Gillies, A. R.; Skretas, G.; Wood, D. W., “Engineered Systems for Detect
ion and
Discovery of Nuclear Hormone
-
Like Compounds,”
Biotechnology Progress
, Vol.
24, pp. 8
-
16, (2008).


Mee, C., Banki, M. R. and Wood, D. W., “Towards the Elimination of
Chromatography in Protein Purification: Expressing Proteins Engineered to Purify
Th
emselves,”
Chemical Engineering Journal
, Vol. 135, pp. 56
-
62, (2008).


Skretas, G., Meligova, A., Villalonga
-
Barber, C., Mitsiou, D. J., Alexis, M.N., Micha
-
Screttas, M., Steele, B.R., Screttas, C.G., & Wood, D.W., “Engineered Chimeric
Enzymes as Tools for

Drug Discovery: Generating Reliable Bacterial Screens for
the Detection, Discovery and Assessment of Estrogen Receptor Modulators,”
Journal of the American Chemical Society
, Vol. 129 (27), pp. 8443
-
8457, (2007).


Wu, W.
-
Y, Mee, C., Califano, F., Banki, R.

& Wood, D.W., “Recombinant Protein
Purification by Self
-
Cleaving Aggregation Tag,”
Nature Protocols
, Vol. 1, pp.
2257
-
2262, (2006).


Skretas, G. & Wood, D.W., “Rapid Detection of Subtype
-
Selective Nuclear Hormone
Receptor Binding with Bacterial Genetic Se
lection,”
Applied and Environmental
Microbiology
, Vol. 71, pp. 8995
-
8997, (2005).


Banki, M.R. & Wood, D.W., “Inteins and Affinity Resin Substitutes for Protein
Purification and Scale Up,”
Microbial Cell Factories
, Vol. 4:32, (2005).


Banki, M.R., Feng, L.

& Wood, D.W., “Simple Bioseparations Using Self
-
Cleaving
Elastin
-
Like Polypeptide Tags,”
Nature Methods
, Vol. 2, pp. 659
-
661, (2005).

http://dx.doi.org/10.1038/nmeth787


Barnard, G.C., McCool, J.D., Wood, D.W. & Gerngross T.U., “An Integrated
Recombinant
Protein Expression and Purification Platform,”
Applied and
Environmental Microbiology
, Vol. 71, pp. 5735
-
5742 (2005).


Banki, M.R., Gerngross, T.U. & Wood, D.W., “Novel and Economical Purification of
Recombinant Proteins: Intein
-
Mediated Protein Purificati
on Using
In Vivo

Polyhydroxybutyrate (PHB) Matrix Association,”
Protein Science
, Vol. 14, pp.
1387
-
1395, (2005).


Bradley, L.H., Kleiner, R.E., Wang, A.F., Hecht, M.H. & Wood, D.W., “An Intein
-
Based Genetic Selection Allows the Construction of a High
-
Quali
ty Library of
Binary Patterned
De Novo

Protein Sequences,”
Protein Engineering Design and
Selection
, Vol. 18, pp. 201
-
207, (2005).


Skretas, G. & Wood, D.W., “A Bacterial Biosensor of Endocrine Modulators,”
Journal of Molecular Biology
, Vol. 349, pp. 464
-
4
74, (2005).


Skretas, G. & Wood, D.W., “Regulation of Protein Activity with Small
-
Molecule
-
Controlled Inteins,”
Protein Science
, Vol. 14, pp. 523
-
532, (2005).


Wood, D., “Simplified Protein Purification Using Engineered Self
-
Cleaving Affinity
Tags,”
Journa
l of Chemical Technology and Biotechnology
, Vol. 78, pp. 103
-
110,
(2003).


Wu, W., Wood, D., Belfort, G., Derbyshire, V. & Belfort, M., “Intein
-
Mediated
Purification of Cytotoxic Endonuclease I
-
TevI by Insertional Inactivation and pH
-
Controllable Splicing,

Nucleic Acids Research
, Vol. 30, pp. 4864
-
71, (2002).


Wood, D., “Conquering the Proteome: Complexity, Cooperation and Commerce,”
Trends in Biotechnology
, Vol. 19, pp. 375
-
376, (2001).


Wood, D., Derbyshire, V., Wu, W., Chartrain, M., Belfort, M. and Bel
fort, G.,
“Optimized Single
-
Step Affinity Purification with a Self
-
Cleaving Intein Applied
to Human Acidic Fibroblast Growth Factor,”
Biotechnology Progress
, Vol. 16,
pp. 1055
-
1063, (2000).


Wood, D., Wu, W., Derbyshire, V,. Belfort, G. and Belfort, M., “A

Genetic System
Yields Self
-
Cleaving Inteins for Bioseparations,”
Nature Biotechnology
, Vol. 17,
pp. 889
-
892, (1999).


Derbyshire, V., Wood, D., Wu, W., Dansereau, J., Dalgaard, J. and Belfort, M.,
“Genetic Definition of a Protein
-
Splicing Domain: Function
al Mini
-
Inteins
Support Structure Predictions and a Model for Intein Evolution,”
Proc. Natl.
Acad. Sci. USA,

Vol. 94, pp. 11466
-
11471, (1997).


EDITED VOLUME

Belfort, M., Derbyshire, V., Stoddard, B.L. & Wood, D.W. (Eds.),
Homing
Endonucleases and Inteins
.

Series: Nucleic Acids and Molecular Biology, Vol. 16,
Springer, Berlin, (2005).


CHAPTERS

Gillies, A., Banki, M. R. & Wood, D. W., “PHB
-
Intein Mediated Protein Purification
Strategy,” in
Methods in Molecular Biology: High Throughput Protein Expression
an
d Purification
, Vol. 498, (ed. Sharon A. Doyle). Humana Press, Totowa, NJ, USA,
(2009).


Gillies, A.G. & Wood, D.W., "Inteins in Protein Engineering," in
Protein Engineering
Handbook
, (eds. Stefan Lutz and Uwe Bornscheuer). Wiley
-
VCH Publishers,
Weinheim,
Germany, (2009).


Wood, D.W. & Skretas, G., “Intein Reporter and Selection Systems,” In
Homing
Endonucleases and Inteins
. Series: Nucleic Acids and Molecular Biology, Vol. 16,
(eds. M. Belfort, V. Derbyshire, B.L. Stoddard & D.W. Wood). Springer, Berlin,
(2005).


Wood, D.W., Harcum, S. & Belfort, G., “Industrial Applications of Intein
Technology,” In
Homing Endonucleases and Inteins
. Series: Nucleic Acids and
Molecular Biology, Vol. 16, (eds. M. Belfort, V. Derbyshire, B.L. Stoddard & D.W.
Wood). Springer
, Berlin, (2005).


PATENTS

US Patent # 6,933,362:

Belfort, G., Belfort, M., Derbyshire, V., Wood, D., and
Wu, W., “Genetic System and Self
-
Cleaving Inteins therefrom, Bioseparations
Employing Same, Protein Purification by Inactivation with Inteins in Linke
r
Region and pH Controllable Intein Splicing, and Methods for Determining
Critical, Generalizable Residues for Varying Intein Activity.”


US Patent #
7,592,144
:

Wood, D. and Skretas, G., “Bacterial Ligand
-
Binding
Sensor.”