Phil-Biotechnology - Winsoft Tech

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12 Φεβ 2013 (πριν από 4 χρόνια και 8 μήνες)

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M.PHIL


BIOTECHNOLOGY


CURRICULUM




Paper


I

RESEARCH METHODOLOGY THEORY AND TECHNIQUES


(Bio Technology, Biochemistry, Botany, Chemistry, Commerce, Computer Science, Education, Electronics, Microbiology, Home Scie
nce,
Library Science, Management, Phy
sics, Psychology, Public Administration, Sociology, Tourism Management, Zoology)

Unit
-

I

Research


Definition


Importance and Meaning of research


Characteristics of research


Types of Research


Steps
in research


Identification, Selection and form
ulation of research problem


Research questions


Research design


Formulation of Hypothesis


Review of Literature.

Unit


II


Sampling techniques : Sampling theory


types of sampling


Steps in sampling


Sampling and Non
-
sampling error


Sample size


Advantages and limitations of sampling.


Collection of Data : Primary Data


Meaning


Data Collection methods


Secondary data


Meaning


Relevances,
limitations and cautions.

Unit


III


Statistics in Research


Measure of Central tendency


Dispersio
n


Skewness and Kurtosis in research.


Hypothesis


Fundamentals of Hypothesis testing


Standard Error


Point and Interval estimates


Important Non
-
Parametric tests : Sign, Run, Kruskal


Wallis tests and Mann
-
Whitney test.

Unit


IV


Para metric tests

: Testing of significance


mean, Proportion, Variance and Correlation


testing for Significance of
difference between means, proportions, variances and correlation co
-
efficient.

Chi
-
square tests


ANOVA


One
-
way and Two
-
way.

Unit


V


Research Report :

Types of reports


contents


styles of reporting


Steps in drafting reports


Editing the final draft


Evaluating the final draft.


Reference Books

1. Statistical Methods





-

S.P. Gupta

2. Research Methodology Methods and Techniques
-

C.R. Kothar
i

3. Statistics (Theory and Practice)



-

B.N. Gupta

4. Research Methodology Methods and Statistical Techniques
-

Santosh Gupta






BIO TECHNOLOGY IN AGRICULTURE

Pa
per

I
I


UNIT 1:

History and scope of Biotechnology. Aim application and role of Biote
chnology in Agriculture,
Forestry, Medical, Industry and human genome development. Future trends and development in
Biotechnology.

UNIT 2:

DNA as genetic material, structure and replication, organization of pro and eukaryotic genome.
Gene expression, c
ontrol and terminator gene technology
-

Western and Northern blotting

UNIT 3:

Direct method of gene transfer technology


electrophoration


micro injection


Plasmid
transformation


Agro bacterium uses


Production of transgenic plants


Gene cloning


Bi
o
reactors


Gene pool


RELP, AFLP, RAPD markers.

UNIT 4:

Biotechnology of some important Crop plants


Rice, Sugarcane and cotton


Micro propagation


Protoplast fusion embryo culture


Synthetic seed


embryo rescue


somatic embryogenesis


someclonal

variation.


UNIT 5:

Biotechnology in animal cell culture
-

Stem cells


Production of vaccines


enzyme


Human
genome concept


PCR Reaction


Gene mapping


Human Disease Control.

REFERENCE

1.

Microbiology


Michael J. Pelezar Jr.E.C.S. Chacor.Noel R. Kri
eg

2.

General Microbilogy


Vol.II


Power & Daginawala

3.

(Eds) Mehta S.L., Lodha, M.L. and Sane, P.V. 1993. Recent Advances in plat
Biochemistry. Publication and information divisions ICAR, New Delhi.

4.

(Eds) D.K. Salunkhe, Kadam S.S and Chavsan J.K. 1985. Po
st harvest
Biotechnology of food legumes. CRC Press, Boca Raton, Florida, USA.

5.

(Eds). Salunkhe D.K, Chavsan J.K. and Kadam S.S (1985). CRC Press, Boca Raton,
Florida Post harves Biotechnology of cereals, USA.

6.

Gupta P.K. Elements of Biotechnology Rastogi.

7.

Chawia H.S. Introduction to Plant Biotechnology, Oxford University press and IBH.

8.

Primrose S.B. Molecular Biotechnology Blackwell Se. Publications


Paper 3


Environmental biotechnology

UNIT


I


Introduction


Goals


Scope


Fundamentals or Biological sy
stem


cells


cell transport through plasma membrane


energetic and respiration


Cell cycle


Fundamentals of genetics


Immune system


triology of environment


basic principles
and population ecology


Food chain, Food web in ecosystem.


UNIT


II


M
olecular modeling


Introduction


Molecular structure and internal energy


application macromolecular modeling


Molecular mechanics and molecular dynamics of oligopeptides, Nucleotides, Proteins, Drug molecules, molecular design


emerging areas in Bioi
nformatics


Bio
-
computing.


UNIT III

Study of microbial population in soil, water, and air:


isolation, screening, and enumeration. Food microbiology


types of
microorganisms in meet, and meat products. vegetables, dairy products; production of baker’s

yeast, and other dairy products.

UNIT IV

Industrial


microbial synthesis of commercial products, organic acids, alcohols, alcoholic beverages and industrial enzymes;
biofertilizers and bioinsecticides. vaccine production from microbes; antibiotics produc
tion.


UNIT V

Waste water treatment


Physical, chemical and biological treatment processes. Activated sludge, oxidation ditches, trickling
filter, towers, rotating discs, rotating drums, oxidation ponds. Anaerobic digestion, anaerobic filters.Bioremediati
on of
contaminated soils and waste lands. Soil waste;
-

composting, vermiculture and methane production


References:

1. Microbial biotechnology, Fundamentals of applied microbiology by A.N.Glazer and

H.Nikaido, W.H.Freeman and Company.

2. P.F.Stanbary and
A.Whittaker, Principles of Fermentation Technology, Pergamann

Press.

3. Casida, Industrial Microbiology

4. Prescott, Industrial Microbiology

5. Alan and Scragg, 1999, Environmental Biotechnology. Pearson Education Ltd.

England.

6. S.N.Jogdand, 1995, Enviro
nmental Biotechnology Himlaya Publishing House Bombay.

7. Waste Water Engineering


Treatment, Disposal and reuse. Metcalf and Eddy, Inc.,

TATA McGraw Hill, New Delhi.

8. A.K.De, Environmental Chemistry Willey Eastern Ltd. New Delhi.

9.D.Allsopp and K.J.Se
al, Introduction to Biodeterioration, ELBS/Edward Arnold.