K - JNU

minerbendΒιοτεχνολογία

12 Φεβ 2013 (πριν από 4 χρόνια και 4 μήνες)

153 εμφανίσεις

Dr. K.J.Mukherjee




EDUCATION

B.Tech, IIT Kanpur (Chemical Engg) 1984,

M.Tech, IIT Delhi (Biochemical Engg) 1987.

Ph.D, IIT Delhi (Biochemical Engg) 1993



RESEARCH


Our research is focused on “Optimization of recombinant prot
ein over expression”. We
use bioprocess strategies in conjunction with genetic ones to get overexpression of a
range of proteins which is achieved by resolving the various bottlenecks that arise in
recombinant protein production, which requires a multi, pr
onged approach. Since we also
work with therapeutically important recombinant proteins these find natural use in
industry.


RECENT PUBLICATIONS


1.

Subramanian Ramalingam, Pennathur Gautam, Krishna Jyoti
Mukherjee

and
Guhan

Jayaraman “
Effects of post
-
induct
ion feed strategies on secretory
production of recombinant streptokinase in
Escherichia coli

.

Biochemical
Engineering Journal
,


January 2007


2.

Poonam Srivastava and
K J Mukherjee
'Kinetic studies of recombinant human
Interferon
-
alpha (rhIFN
-
alpha) expressi
on in transient state continuous cultures.'
Biochemical Engineering Journal, July 2005


3.

Yogender Pal and
K.J. Mukherjee

Production and purification of recombinant
human granulocyte
-
macrophage colony stimulating factor(GM
-
CSF) from high
cell density cultu
res of Pichia pastoris",

Applied Microbiology and
Biotechnology
, June 2005


4.

Palash Bhattacharaya, Gaurav Pandey, Poonam Srivastava,
K.J.Mukherjee

"Combined effect of protein fusion and signal sequence greatly enhances the
production of recombinant GMCSF in

E.coli
" Molecular Biotechnology
June
2005, vol. 30, iss. 2, pp. 103
-
116(14
)


5.

Poonam Srivastava, Palash Bhattacharaya, Gaurav Pandey,
K.J.Mukherjee
,
‘Overexpression and purification of recombinant human Interferon alpha2b in
Escherichia coli
.’ Protein Expr
ession and Purification

Vol 41, Issue 2, June 2005,
Pages 313
-
322


6.

Amardeep Khushoo, Yogender Pal and
K.J. Mukherjee.

“Optimization of
extracellular production of recombinant asparaginase in
Escherichia coli

in shake
flask and bioreactor”. Applied Microbi
ology and Biotechnology . January 2005


7.

Amardeep Kushoo, Yogender Pal, Bairab Nath Singh,
K. J. Mukherjee

Extracellular expression and single step purification of recombinant Escherichia
coli

L

asparaginase II Protein Expression & Purification 38 (2004)
29
-
36


8.

K.J. Mukherjee
, D.C.D. Rowe, N.A. Watkins and D.K. Summers; Studies on
Single Chain Antibody Expression in Quiescent
Escherichia coli

Appl and Env.
Microbiol. (2004), 70: 3005
-
12.


9.

J.C. Gupta and
K.J. Mukherjee
; Stability studies of recombinant
Sac
charomyces
cerevisiae

in the presence of varying selection pressure;
Biotechnol Bioeng.
(2002) Aug 5;79(3):362


10.

S.S. Yazdani and
K.J. Mukherjee
; Continuous culture studies on the stability and
expression of
recombinant streptokinase in
Escherichia coli
;
Bi
oprocess Biosyst
Eng (2002), 24: 341
-
346


11.

Jagdish Gupta and
K.J. Mukherjee
; Stable maintenance of plasmid in continuous
culture of yeast under non
-
selective conditions; J Biosci Bioeng.
(2001):

92(4):


12.

Poonam Srivastava and
K.J. Mukherjee
; Cloning charac
terization and
expression of xylanase gene from
Bacillus lyticus

in
Escherichia coli
and
Bacillus
subtilis
; Prep Biochem and Biotechnol.

(
2001) Nov;31(4):389
-
400


13.

Y. Pal, J.C. Gupta and
K.J. Mukherjee
; Optimizing recombinant protein
expression in the T7 sy
stem under the control of the proUp promoter; Biotech
Lett 23; 41
-
46 (2001)


14.

J.C. Gupta, G. Pandey and
K.J. Mukherjee
; Two stage cultivation of
recombinant
Saccharomyces cerevisiae

to enhance plasmid stability under non
-
selective conditions: Experimental s
tudy and modelling; Enzyme Microbiol
Technol; 28, 89
-
99 (2001)


PROJECTS:

“Development of novel platform for recombinant protein expression using quiescent
cells” under the Indo
-
UK collaboration on Biotechnology”

Department of Biotechnology
,

May 2006 to Ma
y 2008
,

9.9 lacs


“Metabolic Engineering of
E.coli

to remove the bottlenecks in recombinant protein
expression”

Department of Biotechnology,

September2005 to Sept 2008, Rs. 40 Lacs




Development of an integrated bioprocess strategy for the production of
interferon β”,

PRDSF programme of DST June, 2005 to June, 2007 Rs. 105 lacs total (share of JNU =
72.5 lacs )


“Large scale production of antibody fragments in
E.coli

and methylotrophic yeast”

UGC
‘ UPOE” April, 2002 to March, 2007 Rs. 25 lacs



“Process
optimization of Asparaginase production in recombinant
E.coli”

Department of Biotechnology, October, 2000 to September, 2003, 19 Lacs (approx)


“Optimization of Xylanase production using a recombinant
Bacillus

strain


Department
of Biotechnology from

Apr
il, 1997 to October, 2000, Rs.20 Lacs (approx)








CAREER


1991


July 2000: Assistant Professor, Centre for Biotechnology, JNU

August 2000


till date: Associate Professor,

School of Biotechnology, JNU



AWARDS:


Awarded the Commonwealth fellowship for one year at Dept. of Genetics Univ. of
Cambridge from 1
st

Oct 2001 to 30
th

Sept 2002.