Sedimentation Sample Preparation Form

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22 Φεβ 2014 (πριν από 3 χρόνια και 5 μήνες)

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Sedimentation Sample

Preparation F
orm

Please
fill out this form as much as you can and
either
e
-
mail
it to

sfchase@unh.edu

or
mail it to:


Center to Advance Molecular Interaction Science

University of New Hampshire

Rudman Hall 379

46 College Road

Durham, New Hampshire 03824

I. Contact Information:

Contact
Name:


Address:


City:


State:


Zip code:


E
-
mail address:


Telephone:



II. Sample Information:

If
you have less than 3 samples, leave the corresponding fields bl
ank:






Sample 1

Sample 2

Sample 3

Brief Description of
Sample




Approx. Molecular
Weight




Detection system (Int.,
FDS, or Abs

and wavelength if
Abs)




Buffer components
and concentrations




OD of Sample vs.
Buffer




OD of Buffer vs.
Wate
r




Partial Spec. Volume
(V
-
bar)





Density of Buffer rel.
to H
2
O at 20C




Viscosity of Buffer rel.
to H
2
O at 20C




Run Temperature




Storage conditions
(temperature)




Special Notes

(handling or anything

else we need to know)




Amino Acid S
equence, Sample 1:


Please
write

a description for the amino acid sequence of sample 1:







Please enter the amino acid sequence of sample 1 (single letter code):










Amino Acid Sequence, Sample 2:


Please enter a description for the amino acid sequ
ence of sample 2:







Please enter the amino acid sequence of sample 2 (single letter code):

































Amino Acid Sequence, Sample 3:


Please
write
r a description for the amino acid sequence of sample 3:







Please write

the amino

acid sequence of sample 3 (single letter code):






















Please
bold or check YES to indicate that you have read the Chemical Compatibility
Table
:

Yes

No

I have read t
he
Chemical Compatibility Table

(below
)
and understand
that I am liable for any damages caused by failing to identify
damaging chemicals in my buffer system


Chemical Compatibility Table

Chemical
Group:

Anodized
Aluminum

Charcoal/
Epoxy

Description:

Acids

(aq):

S

S

acetic acid (5%)/ethanoic acid

Acids (aq):

S

S

acetic acid (60%)/ethanoic acid

Acids (aq):

S

S

acetic acid (glacial)/ethanoic acid

Acids (aq):

S

S

boric acid

Acids (aq):

S

U

chromic acid (10%)

Acids (aq):

S

S

citric acid/2
-
hydroxy
-
1,2,3
-
pro
panetricarboxylic acid

Acids (aq):

U

S

hydrochloric acid (10%)

Acids (aq):

U

U

hydrochloric acid (50%)

Acids (aq):

S

S

iodoacetic acid/2
-
iodoethanoic acid

Acids (aq):

S

M

mercaptoacetic acid/2
-
mercaptoethanoic
acid

Acids (aq):

S

U

nitric acid (10%)

A
cids (aq):

S

U

nitric acid (50%)

Acids (aq):

-

S

oleic acid/cis
-
9
-
octadecenoic acid

Acids (aq):

U

S

oxalic acid/ethanedioic acid

Acids (aq):

U

1

perchloric acid (70%)1

Acids (aq):

U

S

phosphoric acid mixture (10%)

Acids (aq):

U

S

phosphoric acid mixtu
re (50%)

Acids (aq):

S

M

picric acid/2,4,6
-
trinitrophenol

Acids (aq):

-

-

saturated fatty acids (see stearic acid)

Acids (aq):

-

S

stearic acid/octadecanoic acid

Acids (aq):

U

S

sulfosalicylic acid/3
-
carboxy
-
4
-
hydroxybenzenesulfonic acid

Acids (aq):

U

U

sulfuric acid (10%)

Acids (aq):

U

U

sulfuric acid (50%)

Acids (aq):

-

-

thioglycolic acid (see mercaptoacetic acid)

Acids (aq):

U

S

trichloroacetic acid/trichloroethanoic acid

Acids (aq):

-

-

unsaturated fatty acids (sea oleic acid)




Chemical
Anodized
Charcoal/ Epoxy

Description:

Gr
oup:

Aluminum

Bases (aq):

U

S

ammonium hydroxide (10%)

Bases (aq):

U

U

ammonium hydroxide (28%)

Bases (aq):

S

U

aniline/benzenamine

Bases (aq):

U

S

potassium hydroxide (5%)

Bases (aq):

U

S

potassium hydroxide (45%
)

Bases (aq):

S

U

pyridine (50%)/azabenzene

Bases (aq):

U

S

sodium hydroxide (1%)

Bases (aq):

U

S

sodium hydroxide (>l%)

Chemical
Group:

Anodized
Aluminum

Charcoal/
Epoxy

Description:

Salts (aq):

U

S

aluminum chloride

Salts (aq):

S

S

ammonium acetat
e/ammonium ethanoate

Salts (aq):

S

S

ammonium carbonate

Salts (aq):

-

S

ammonium phosphate

Salts (aq):

M

S

ammonium sulfate

Salts (aq):

U

S

barium salts

Salts (aq):

U

S

calcium chloride

Salts (aq):

U

S

guanidine hydrochloride/l
-
aminomethanamidine hyd
rochloride

Salts (aq):

S

S

magnesium chloride

Salts (aq):

S

S

nickel salts

Salts (aq):

S

S

potassium bromide

Salts (aq):

U

S

potassium carbonate

Salts (aq):

S

S

potassium chloride

Salts (aq):

S

S

potassium permanganate

Salts (aq):

S

S

silver nitrate

Salts (aq):

S

S

sodium borate

Salts (aq):

U

S

sodium carbonate

Salts (aq):

U

S

sodium chloride

Salts (aq):

S

S

sodium nitrate

Salts (aq):

S

S

sodium sulfate

Salts (aq):

S

S

sodium sulfite

Salts (aq):

U

S

zinc chloride

Salts (aq):

S

S

zinc sulfate

Chemical Group:

Anodized
Charcoal/
Description:

Aluminum

Epoxy

Gradient Forming Materials (aq):

-

S

cesium acetate/cesium
ethanoate

Gradient Forming Materials (aq):

S

S

cesium bromide

Gradient Forming Materials (aq):

S

S

cesium chloride

Gradient Form
ing Materials (aq):

S

S

cesium formate/cesium
methanoate

Gradient Forming Materials (aq):

S

S

cesium iodide

Gradient Forming Materials (aq):

S

S

cesium sulfate

Gradient Forming Materials (aq):

S

S

dextran or dextran sulfate

Gradient Forming Materials (
aq):

S

S

Ficoll
-
Paque

Gradient Forming Materials (aq):

S

S

glycerol/1,2,3
-
propanetriol

Gradient Forming Materials (aq):

S

S

metrizamide

Gradient Forming Materials (aq):

S

S

rubidium bromide

Gradient Forming Materials (aq):

S

S

rubidium chloride

Gradie
nt Forming Materials (aq):

S

S

sodium bromide

Gradient Forming Materials (aq):

S

S

sodium iodide

Gradient Forming Materials (aq):

S

S

sucrose/beta
-
D
-
fructofuranosyl
-
alpha
-
D
-
glucopyranoside

Gradient Forming Materials (aq):

S

S

sucrose, alkaline/beta
-
D
-
fr
uctofuranosyl
-
alpha
-
D
-

Gradient Forming Materials (aq):



glucopyranoside

Chemical
Group:

Anodized
Aluminum

Charcoal/
Epoxy

Description:

Solvents:

S

U

acetone/2
-
propanone3

Solvents:

S

M

acetonitrile/ethanenitrile3

Solvents:

S

U

benzene
3

Solvents:

1

U

carbon tetrachloride/tetrachloromethane

Solvents:

1

S

chloroform/trichloromethane

Solvents:

S

U

cresol mixture/methylphenol

Solvents:

S

S

cyclohexane3

Solvents:

S

S

diethyl ether/ethoxyethane3

Solvents:

-

M

diethyl ketone/3
-
pentanone3

Solvents:

S

M

N,N
-
dimethyiformamide/N,N
-
dimethylmethanamide3

Solvents:

S

S

dimethyl sulfoxide/sulfinylbis(methane)

Solvents:

S

S

dioxane/1,4
-
dioxacyclohexane3

Solvents:

S

S

ethanol (50%)3

Solvents:

S

S

ethanol (95%)3

Solvents:

-

-

ether (see diethyl

ether)

Solvents:

S

S

ethyl acetate/ethyl ethanoate3

Solvents:

S

S

ethylene glycol/ 1,2
-
ethanediol

Solvents:

S

S

hexane3

Solvents:

S

S

isopropyl alcohol/2
-
propanol3

Solvents:

S

S

kerosene3

Solvents:

S

S

methanol3

Solvents:

1

S

methylene chloride/dic
hloromethane

Solvents:

S

S

methyl ethyl ketone/2
-
butanone3

Solvents:

S

M

phenol (5%)

Solvents:

S

U

phenol (50%)

Solvents:

S

U

tetrahydrofuran3

Solvents:

S

S

toluene/methylbenzene3

Solvents:

S

S

water

Solvents:

S

S

xylene mixture/dimethylbenzene3

Chemical
Group:

Anodized
Aluminum

Charcoal/
Epoxy

Description:

Detergents:

U

S

Alconox

Detergents:

S

S

deoxycholate, sodium dodecyl sulfate, Triton
X
-
100

Detergents:

S

S

Haemo
-
Sol

Detergents:

S

S

Solution 555Tm (20%)

Detergents:

S

S

Zephiran chlori
de (1%)

Detergents:

-

-

dibutyl phthalate (see n
-
butyl phthalate)

Detergents:

S

S

diethyl pyrocarbonate/pyrocarbonic acid
diethyl ester

Detergents:

-

U

ethylene oxide vapor4/oxirane3

Detergents:

S

S

formaldehyde/methanal

Detergents:

-

-

formalin (40%
) (see formaldehyde)

Detergents:

U

U

hydrogen peroxide (3%)

Detergents:

U

U

hydrogen peroxide (10%)1

Detergents:

S

M

2
-
mercaptoethanol3

Detergents:

S

S

oils (petroleum)

Detergents:

-

S

oils (other)

Detergents:

S

S

physiologic media (e.g., culture med
ia, milk,
serum, urine)

Detergents:

S

M

sodium hypochlorite

Detergents:

S

S

Tris buffer (neutral
pH)/tris(hydroxymethyl)aminomethane

Detergents:

S

S

urea

Chemical
Group:

Anodized
Aluminum

Charcoal/
Epoxy

Description:

Other:

S

S

n
-
butyl phthalate4/dib
utyl 1,2
-
benzenedicarboxylate






Legend:

S

satisfactory resistance

M

marginal resistance

U

unsatisfactory resistance

1

explosion hazard due to possible material/chemical reaction under rotor failure
conditions

2

OK below 26º C only

3

Flammability h
azard. Not recommended for use in any type of centrifuge because
vapors may be ignited by exposure to electrical contacts. Depending on the
centrifuge type, such exposure could occur either during normal centrifugation or
under failure conditions.

4

nonaq
ueous

-

Unknown