GENE TRANSFER AND GENETIC ENGINEERING

igocheddarΒιοτεχνολογία

14 Δεκ 2012 (πριν από 4 χρόνια και 10 μήνες)

140 εμφανίσεις

1

GENE TRANSFER AND
GENETIC ENGINEERING

CHAPTER 8

2

Gene Transfer


Vertical
to offspring




Lateral (horizontal)

to others in same generation

3

Bacterial Gene Transfer and
Recombinant DNA Technology


Transformation


Griffith’s
experiment






4

Transformation


Mechanism





Significance


Recombination?

5

Transduction


Mechanism





Significance

Transducing phages:

p1, p22

Can be used in genetic mapping

http://jb.asm.org/cgi/reprint/174/23/7876

6

Conjugation


Mechanism





Significance

Also used for mapping

Conjugation pilus

7

Plasmids


F plasmid
(conjugation)



Resistance plasmids



Virulence plasmids

8

Plasmids (cont.)


Tumor
-
inducing plasmids



Agrobacterium tumefaciens and plants




Plasmids conferring novel catabolism

Degradation pathways on a plasmid

9

Genetic Engineering


Genetic fusions



reporter fusions



promoter fusions




10

Genetic Engineering


Protoplast fusion


electroporation

11

Genetic Engineering (cont.)


Gene
Amplification





Recombinant
DNA cloning

12

Applications


Medical



Industrial



Agricultural



Hybridomas

13

Risks and Benefits

Frankenstein?


Frankenfoods?

14

Recombinant DNA technology


Plasmids
-

autonomous replicating extrachromasomal
DNA


restriction enzymes

15

Viral technology
-

phage cloning

16

Homologous recombination

Used to make gene

knockouts in bacteria and

yeast

17

Transposons
-

jumping genes

18

Evidence for extensive
transfer of antibiotic
resistance genes in

Bacteroides

sp.

http://mcb.illinois.edu/faculty/profile/abigails