Ozone Cancer Studies Table of Contents

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Ozone Cancer Studies

Table of Contents

Ozone Selectively Inhibits Growth of Human Cancer Cells

Ozone Therapy for Tumor
Oxygenation: a Pilot Study

Ozone Plus Cobalt Therapy In Patients Suffering From Prostatic Cancer

Adjuvant Ozone therapy in Adv
anced Head and Neck Tumors: A Comparative Study

Effect of a parenteral ozone
oxygen mixture on the concentration of immunoglobulins (IgA, IgG,
IgM), of vitamin A and lysozyme activity in patie
nts with cervical cancer

Intravenous injection therapy with an ozone
oxygen mixture in gynecological radiotherapy

Ozone and gyn
ecologic radiotherapy

Cancer outcomes at the Hufeland: a best
case series review

Ozone therapy in a Complex Treatment of Breast


Three case reports on the holistic approach to patients with neoplasm including the use of
medical ozone

en Therapy for Gynecologic Carcinomas

Ozonotherapy in the Early Postoperative Period in the Surgical Treatment of Lung Cancer

In Vitro Synergistic Activity of 5
fluorouracil with Low
dose Ozone Against a Chemoresistant
Tumor Cell Line and Fresh Human Tumor Cells

Restoration of Normoxia

by Ozone Therapy May Control Neoplastic Growth

Study Extracts:

1. Ozone Selectively Inhibits Growth of Human Cancer Cells

From Science magazine Vol. 209, 22 Aug 1980, pp. 931


The growth of human cancer cells from lung, breast, and uterine
tumors was selectively inhibited in
a dose
dependent manner by ozone at 0.3 to 0.8 parts per million of ozone in ambient air during 8
days of culture. Human lung diploid fibroblasts served as noncancerous control cells. The presence
of ozone at 0.3 to 0.5
parts per million inhibited cancer cell growth 40 and 60 percent, respectively.
The non
cancerous lung cells were unaffected at these levels. Exposure to ozone at 0.8 parts per
million inhibited cancer cell growth more than 90 percent and control cell grow
th less than 50
percent. Evidently, the mechanisms for defense against ozone damage are impaired in human
cancer cells.

Ozone Therapy for Tumor Oxygenation: a Pilot

Authors: Bernardino Clavo1; Juan L. Pérez2; Laura López1; Gerardo Suárez1; Marta Lloret1; Victor
Rodríguez3; David Macías2; Maite Santana1; María A. Hernández1; Roberto Martín
Oliva2; Francisco

Source: Evidence
based Complementary and

Alternative Medicine, Volume 1, Number 1, 01 June
2004, pp. 93
98(6) Publisher: Oxford University Press


Tumor hypoxia is an adverse factor for chemotherapy and radiotherapy. Ozone therapy is a non
conventional form of medicine that has been used successfully in the treatment of ischemic
disorders. This prospective study was designed to assess the effect of o
zone therapy on tumor
oxygenation. Eighteen subjects were recruited for the study. Systemic ozone therapy was
administered by autohemotransfusion on three alternate days over one week. Tumor oxygenation
levels were measured using polarographic needle probe
s before and after the first and the third
ozone therapy session. Overall, no statistically significant change was observed in the tumor
oxygenation in the 18 patients. However, a significant decrease was observed in hypoxic values 10
and 5 mmHg of pO<inf>
2</inf>. When individually assessed, a significant and inverse non
correlation was observed between increase in oxygenation and the initial tumor pO<inf>2</inf>
values at each measuring time
point, thus indicating that the more poorly
oxygenated tum
benefited most (rho =
0.725; P = 0.001). Additionally, the effect of ozone therapy was found to be
lower in patients with higher hemoglobin concentrations (rho =
0.531; P < 0.034). Despite being
administered over a very short period, ozone therapy im
proved oxygenation in the most hypoxic
tumors. Ozone therapy as adjuvant in chemo
radiotherapy warrants further research.

Document Type: Research article

DOI: 10.1093/ecam/neh009

Affiliations: 1: Radiation Oncology and Research Unit, Las Palmas (Canary
Islands), Spain 2: Medical
Physics, Las Palmas (Canary Islands), Spain 3: La Paterna Medical Center, Las Palmas (Canary Islands),
Spain 4: Chronic Pain Unit, Dr Negrín Hospital, Las Palmas (Canary Islands), Spain

3. Ozone Plus Cobalt Therapy In Patients S
uffering From Prostatic Cancer

Reported at the 2nd International Symposium on Ozone Applications: March 1997 by L. Borrego, L. L.
Borrero, E. C. Díaz1, S. Menéndez2, L. R. Borrego3, R. A. Borrego3. This study involved these
institutes in Cuba: Provincial
Center of Retinitis Pigmentosa, Holgu'n, V.I. Provincial Center of
Retinitis Pigmentosa, Holguín, Ozone Research Center.

Prostatic adenocarcinoma is the most frequent tumor in men older than 50 years old, representing
the 16.7 % of all the death cases of
cancer in male sex. With an early diagnose and an appropriate
treatment the survival increase in 5 years, in 91 % of cases. Cancer metabolism is fundamentally
anaerobic, for that reason, increasing oxygenation in damaged tissues, may diminished the side
fects produced by radiation treatments, as well as increase radio
sensibility. The aim of this study
is to evaluate the efficacy of ozone therapy in conjunction with cobalt
60 therapy, in the treatment
of patients suffering from prostatic cancer, taking in
to account the influence that ozone exerts in
tissue oxygenation. 70 patients with malignant neoplasia in prostate, stage A and B of the disease
(intracapsular) were treated by an oncourology muldisciplinary group of the "Lenin" Hospital
(Holguin Province)
. 35 patients were treated with rectal ozone plus cobalt therapy (ozone group)
and 35 with cobalt therapy only (control group). The appearance of side effects (dermatitis radiation,
cystitis, proctitis) and the behavior of some humoral and clinical paramet
ers were evaluated up to 6
months after finishing the treatment. 84 % and 52 % of side effects were presented in control group
and ozone group, respectively. Prostatic specific antigen (PSA) decrease, less than 10 ng/mL, in 92 %
of patients treated with oz
one and in 52 % in control group,

one month after finishing the treatment. 88 % and 80 % of clinical and humoral control of the
disease were obtained in ozone group and control group, respectively, 6 months later. It is
concluded that, combining cobalt th
erapy with ozone, a decrease in side effects and an increase in
sensibility were achieved.

Adjuvant Ozone Therapy in Ad
vanced Head and Neck Tumors: A Comparative Study


Clavo B, Ruiz A, Lloret M, Lopez L, Suarez G, Macias D, Rodriguez V, Hernandez MA, Martin
Oliva R,
Quintero S, Cuyas JM, Robaina F.

Advanced head and neck (H&N) tumors have a poor prognosis, and this

is worsened by the
occurrence of hypoxia and ischemia in the tumors. Ozone therapy has proved useful in the
treatment of ischemic syndromes, and several studies have described a potential increase of
oxygenation in tissues and tumors. The aim of this pros
pective study was to evaluate the clinical
effect of Ozone therapy in patients with advanced H&N cancer in the course of their scheduled
radiotherapy. Over a period of 3 years, 19 patients with advanced H&N tumors who were
undergoing treatment in our depar
tment with non
standard fractionated radiotherapy plus oral
tegafur. A group of 12 patients was additionally treated with intravenous chemotherapy before
and/or during radiotherapy. In the other group of seven patients, systemic ozone therapy was
red twice weekly during radiotherapy. The ozone therapy group was older (64 versus 54
years old, P = 0.006), with a higher percentage of lymph node involvement (71% versus 8%, P =
0.019) and with a trend to more unfavorable tumor stage (57% versus 8% IVb +

IVc stages, P =

0.073). However, there was no significant difference in overall survival between the chemotherapy
(median 6 months) and ozone therapy (8 months) groups. Although these results have to be viewed
with caution because of the limited number o
f patients, they suggest that ozone therapy could have
had some positive effect during the treatment of our patients with advanced H&N tumors. The
adjuvant administration of ozone therapy during the chemo
radiotherapy for these tumors merits
further resear

PMID: 15841266 [PubMed

as supplied by publisher]

Effect of a parenteral ozone
oxygen mixture on the concentr
ation of immunoglobulins (IgA, IgG,
IgM), of vitamin A and lysozyme activity in patients with cervical cancer

Geburtshilfe Frauenheilkd. 1987 Dec;47(12):343
5 written in German

Enzelsberger H
Metka M
Salzer H

Frauenklinik Wien.

In the literature you can find several therapeutic possibilities in the treatment of carcinoma by
additive ozone therapy. We investigated the consequences of ozone
therapy for the immunological
status, lysozyme and vitamin A. 21 women with progressive cervical cancer (Stage III, IV) got besides
the conventional irradiation therapy also an additive ozone therapy. After irradiation and ozone
therapy a small decrease in

IgG, IgA and IgM can be seen. A statistical significance could not be
evaluated. There was no difference to the control group in lysozyme and vitamin A.

PMID: 3436505 [PubMed

indexed for MEDLINE]

Intravenous injection therapy for genital cancer with an ozone
oxygen mixture in gynecological

Injektionsbehandlung in der gynäkologischen Strahlentherapie

Hernuss, P.; E. Müller
Tyl & J. Dimop

Strahlentherapeutische Klinik der Universität Wien, A
1097 Wien, Alserstraße 4, Austria.

Strahlentherapie 148(3): 242
245, 1974.

Written in German


40 female patients with primary and 5 patients with recurrence of genital cancer after tr
have received radiotherapy and a daily parenteral ozone mixture medication. It appears that under
influence of the supplementary ozone
oxygen mixture treatment, the regression of female genital
tumors in both groups studied was faster. The side
ects of the radiotherapy were reduced and the
general condition of the patients improved. The best results were observed in patients with
recurrence of poorly oxygen
supplied genital tumors.

Ozone and gynecologic radiotherapy

Original study written in German and titled: Ozon und gynakologische Strahlentherapie

Hernuss P; Muller
Tyl E; Wicke L

Strahlentherapie (1975 Nov), 150(5), 493
9. ISSN:0039

PubMed ID 1216


A short survey is given of the historical development and the physical basis of ozone therapy. Ozone
is used in medicine as well as in other spheres. Papers reporting good results of ozone treatment in
carcinoma seemed of particular interest. The efficacy
of ozone as an adjuvant to the irradiation of
carcinosarcomas of rats was confirmed by us. On account of this fact ozone was introduced by us as
an adjuvant to the irradiation of women with gynaecological cancer and appeared to give good
results. The mecha
nism of action of ozone is not yet fully clarified and several theories are discussed.
Investigations are currently being undertaken in respect to the behaviour of several substances in
the organism during ozone therapy.

Cancer outcomes at the Hufeland: a best
case series review


(the Hufeland is a complementary/alternative medicine institute)

Integr Cancer Ther
. 2005 Jun;4(2):156

Jacobson JS, Grann VR, Gnatt MA, Hibshoosh H, Austin JH, Millar WS, Neugut AI.

Department of Epidemiology, Mailman School of Public Health, New York, NY 10032, USA.

A best
case series review is an efficient tool with which to scr
een complex complementary and
alternative treatments for cancer as candidates for further study. STUDY DESIGN: The National
Cancer Institute and other agencies have adopted the best
case series method to evaluate cancer
treatments involving complementary a
nd alternative medicine (CAM) for further study. The authors
conducted a best
case series review of the Hufeland Klinik. Established in 1985 in Bad Mergentheim,
Germany, this facility treats more than 500 cancer patients per year. Hufeland treatment includ
dietary modification, injections, ozone therapy, active fever therapy, psychotherapy, and sometimes
hormone therapy and/or low
dose chemotherapy. The goal of the treatment is to prolong survival
and to maintain good quality of life. METHODS: The clinic
provided summaries of 27 cases in which
patients with longer than expected survival had agreed to make their medical records available for
review. The review involved pathologic confirmation of disease and radiologic confirmation of
complete response (CR)
or partial response (PR) not attributable to conventional treatment.
RESULTS: Based on the summaries and an exhaustive 2
year search for medical records, slides, and
imaging data, 12 of 27 cases were selected for full review, and 5 (3 CRs and 2 PRs) were j
udged best
cases. CONCLUSION: Most patients with common cancers receive conventional treatment before
coming to Hufeland, and many are treated with chemotherapy and/or hormonal therapy while
there. Hence, only a few could be considered for review. With 5 o
f 12 patients showing a treatment
response, the authors conclude that the Hufeland treatment merits further study. They also
recommend the development of criteria with which to evaluate best
case series reviews of complex
CAM treatments for patients with a
dvanced cancer.

PMID: 15911928 [PubMed

indexed for MEDLINE]


therapy in a Complex Treatment of Breast Cancer

Kontorschikova, Claudia N.1; Anna V. Alaysova & Igor G
. Terentiev.

Chair of clinical and and Laboratory Diagnostics, Department of Oncology, Nizhni Novgorod State
Medical Academy, 10/1 Minin sq., N. Novgorod, 603005, Russia.

In: Proceedings of the 15th Ozone World Congress, 11th

15th September 2001, Medic
al Therapy
Conference (IOA 2001, Ed.), Speedprint Macmedia Ltd, Ealing, London, UK, 2001.


There have been followed up 52 women with breast cancer, confirmed histologically, age ranging
from 40 to 60 years. 32 patients along with cytostatic thera
py have undergone a course of ozone
therapy of intravenous infusions or rectal insufflations and ozonated water per os. 20 women were
on conventional polychemotherapy. The groups were compatible according the age, stage of the
disease and accompanying path
ology. Involvement of ozone therapy in a complex treatment of
patients with breast cancer helped to diminish the incidence and degree of cytostatics toxical side
effects, improve their life quality and immunological parameters and significally increase the

of antioxidant defence system.

Three case reports on the holistic approach to patients with neoplasm including the use of
medical ozone

Thomas Marshall
Manifold, Wimbledon Clinic of Natural Medicine, London, UK.

In this report we present the successful treatment of three patients with malignant carcinoma with a
combined alternative therapy including Ozone, diet, Homoeopathy, herbs and immunotherapy.
ough treatment with Ozone is not a very aggressive procedure as compared to a large number of
conventional measures, usually other traditional alternative therapies such as Homoeopathy and
herbs are first given to our patients. Then, the patient's response

is evaluated and Ozone is added in
many cases to the initial treatment scheme. Although positive tests for acid phosphatase were never
found in our male patient, P.S.A. levels were highly suggestive of prostate adenocarcinoma which
clearly responded to th
erapy on two occasions. There is a lot of controversy now on whether the
benefits of aggressive treatment in prostate cancer outweigh the associated risks and side effects.
Conventional aggressive treatment include radiation and surgery. A fair percentage
of patients who
undergo this kind of treatment suffer from complications such as impotency (30
50%), mild to
severe incontinence and even death.13 In the case of a residual ovarian tumour, the recovery of the
patient and the return of her lab tests, in par
ticular CA 12.5, to normal values, were clearly
associated to the administration of a combined treatment including Ozone as a major component. In
our patient with breast cancer, there was a dramatic change in the regional histopathology including
the unusu
al reversion of a metastatic tumour process to a localised disease. This event could only be
explained by the intensive therapeutic measures applied soon after the initial pathologic report. It is
also remarkable the way the patient responded to a new trea
tment programme when a new lump
had emerged. These events clearly challenge usual protocols adopted for the conventional
treatment of cancer. It is not unusual to obtain similar satisfactory results with this alternative
treatment programme in other cases
of cancer patients in our clinic. However, an appropriate
documentation of these cases implies a big economic effort from our patients which unfortunately
limits the frequency of clinical reporting. Likewise, it is really difficult to determine the efficie
ncy of
Ozone as a single therapeutic measure in cancer patients. It would obviously be unethical to deprive
these patients of empirically and even scientifically tested alternative measures such as dietary
supplementation in order to establish the real pot
ential of this gas in Oncology.

oxygen Therapy for Gynecologic Carcinomas

The effect of parenteral
ozone oxygen mix
ture administration on free fatty acids and triglycerides in
patients with gynecologic carcinomas

Fortschr Med. 1979 Mar 15;97(10):451

Original article written in German

Tyl E, Salzer H, Reisinger L, Washuttl J, Wurst F.


As some aut
hors suspect that ozone influences the metabolic process of fat, we tried to analyse the
influence of an ozone
oxygen gas which was applied parenterally. 40 patients with gynecological
cancer received 10 ml ozone
oxygen gas with a content of 450 gamma ozon
e and venous blood was
removed before and 10 minutes after application. The serum was lyophilized and the level of fatty
acids and triglycerids was determined by the method of Randerath (1965). a statistically significant
decrease of the concentrations was

observed after application of ozone. Different theories as to the
cause of this action are discussed.

PMID: 369965 [PubMed

indexed for MEDLINE]

Ozonotherapy in the E
arly Postoperative Period in the Surgical Treatment of Lung Cancer

V.P. Potanin, V.M. Belopukhov, R.M. Taziev, M.Kh. Mustafin, I.D. Khalimov


The ozonotherapy

influence on the postoperative treatment in patients after the radical lung
resection due to the lung cancer is analyzed. The studies were performed from 1996 to 1999. The
patients were divided into two group: the patients of the first group (40 persons)
were treated with
the standard infusion therapy in the postoperative period, the patients of the

second group (160
persons) were

treated with ozoned crystalloids combined with the standard therapy. Due to the
ozonotherapy the purulent complications in the
second group reduced by a factor of 12,5 and
mortality reduced by a factor of 2,6.

In Vitro Synergistic Activity of 5
rouracil with Low
dose Ozone Against a Chemoresistant
Tumor Cell Line and Fresh Human Tumor Cells

Chemotherapy. 1990;36(2):147

Zanker KS, Kroczek R., Institute of Immunology and Experimental Surgery, University of
Witten/Herdecke, FRG.


We followed the concept that the chemical reactivity of ozone depends upon its oxidative
properties. Activated oxygen species are causally involved in toxicity of certain chemotherapeutic
drugs. We have tested this prediction in human cell cultures eithe
r to overcome chemoresistance
and/or to increase chemical cytotoxicity. Our results indicate that ozone in combination with 5
fluorouracil (5
FU) makes a 5
resistant cell line susceptible for the combined treatment modality.
Furthermore, ozone acts syne
rgistically or at least additive to chemotherapy in different tumor cell
suspensions, derived from the breast and the colon.

PMID: 2311442 [PubMed

indexed for MEDLINE]

Restoration of Normoxia by Ozone Therapy May Control Neoplastic Growth

J Altern Complement Med. 2005 Apr;11(2):257

Bocci V, Larini A, Micheli V.

Department of Physiology, University of Siena, Italy.



In contrast to normal tissues, tumors thrive in hypoxic environments. This appears to be because
they can metastasize and secrete angiopoietins for enhancing neoangiogenesis and further tumor
spread. Thus, during chronic ischemia
, normal tissues tend to die, while neoplasms tend to grow.
During the past two decades, it has been shown in arteriopathic patients that ozonated
autohemotherapy is therapeutically useful because it increases oxygen delivery in hypoxic tissues,
leading to

normoxia. Although several oxygenation approaches have been tested, none is able to
restore normoxia permanently in patients with cancer. We postulate that a prolonged cycle of
ozonated autohemotherapy may correct tumor hypoxia, lead to less aggressive tu
mor behavior, and
represent a valid adjuvant during or after chemo

or radiotherapy. Moreover, it may re
the chronic oxidative stress and reduce fatigue.

Quantity of Papers

There is a very large number of papers on ozone. The above 14 are onl
y the tip of the iceburg.
Searching in PubMed for ozone returns over 8,000 items.

Conclusiveness of Papers

The above 14 studies are probably the most impressive studies of ozone and cancer. Take the time
to look them over and you will have the best feel f
or how conclusive ozone studies have been.