Genetic Manipulation of Microorganisms

hollandmercifulΒιοτεχνολογία

11 Δεκ 2012 (πριν από 4 χρόνια και 11 μήνες)

196 εμφανίσεις


UIB

U

n

i

v

e

r

s

i

t

a

t



d

e



l

e

s

I

l

l

e

s



B

a

l

e

a

r

s



Master in Microbiology


COURSE DESCRIPTION

2006
-
2007 Academic Year


Technical information

Course

Course title: Genetic Manipulation of Microorganisms

Course code:
a cumplimentar por el Centro de Tecnologías de la Informació
n

Ty
pe of course: Optional

Level of course: Postgraduate

Year of study: First

Semester: First

Timetable: See the timetable for the Master in Microbiology programme

Language of instruction: Spanish/Catalan, reading comprehension skills in
English required

Lect
urers

Supervising lecturer

Name: Dr. Elena García
-
Valdés


Contact:
dbaegv0@uib.es


Other lecturers

Name:






Contact:

Name:






Contact:

Name:






Contact:

Name:






Contact:

Prerequisites:

A solid background (a
t the b
achelor’s degree level) in biochemistry, molecular biology,
genetics, cellular biology, microbiology, plant
-
animal physiology and chemistry

Number of ECTS credits 8

Number of classroom hours: 52

Independent study hours: 148

Description:

Microbial g
enetics. The foundations of genetic engineering in microorganisms. Basic
and advanced cloning techniques in prokaryote and eukaryote microorganisms

Course competences

Specific:

E9
-

Understand the role of microorganisms in the development of recombinant DNA

techniques, their influence in the development of biological concepts and their
biotechnological applications

E10
-

Acquire a practical grounding in the different application
s of recombinant DNA
technology


Generic:

G1
-

Acquire an integrated view of microo
rganisms, their biological properties and their
roles and applications in the fields of ecology, health, industry, agriculture and
biotechnology


G2
-

To
e
nhance
prior
knowledge

of microbiology and the elements involved in
developing and/or applying ideas a
t the research level as well as in other areas


Course contents

1
-
General features of the prokaryote genome. Size, structure, organisation and
functioning. Genomics today.

2
-

Genetic engineering. Introduction: Concept of cloning. Legal definition of geneti
c
manipulation. General overview of the cloning process. Stages. Cloning objectives.

3
-

DNA and cDNA: Initial material in a cloning process.

4
-

Cloning with plasmid vectors. General characteristics. Plasmids in genetic
engineering.
-
Cloning vectors in

E. c
oli
. First generation plasmids: pBR22.
Characteristic origin. Second
-
generation plasmids: pUC and derivatives.

5
-
Restriction enzymes. Definition.
Significance in nature.
T
ypes
. Nomenclature.
Practical applications.

6
-
Union of DNA molecules. Strategies: Ho
mopolymer strings.
Adaptors, d
ecam
eric

links. Alkaline phosphatase. DNA Ligases.

7
-
Transformation methods. Concept. Protocol competent cells. Conjugation,
Transfection in genetic engineering.

8
-
Selection of cloned genes. Direct methods. Examples. Indirect
methods. Probes.
Example of marking non
-
radioactive probes. Examples. Indirect methods.
Antibodies.

9
-
Cloning the somatostatin gene.

10
-
Other cloning vectors: Viral vectors. Introduction: life cycle of the phage lambda.
Genetic organisation of the phage l
ambda. Insertion and replacement vectors.
Different viral vectors.
Cosmids
. Genomic libraries.

11
-
Other cloning vectors: Expression vectors. Introduction: Molecular signals that
affect gene expression. Definition of expression vectors. Examples.

12
-

Metho
ds for nucleic acid extraction and purification


Methodology and student workload

1.

Teaching method: Classroom sessions

Classroom/independent work: 15/15

E
-
learning: yes

Type of group: whole group

2.

Teaching method:
Practical l
aboratory

work

Classroom/indepe
ndent work: 10/20

E
-
learning: yes

Type of group: individual

3.

Teaching method:
Supervised l
aboratory

work

Classroom/independent work: 15/30

E
-
learning: yes

Type of group: individual

4.

Teaching method: Group work presentations (seminars)

Classroom/independent

work: 4/8

E
-
learning: yes

Type of group: groups of 2/3, whole group presentation

5.

Teaching method: Tutorials

Classroom/independent work: 4/0

E
-
learning: no

Type of group: groups of four

6.

Teaching method: Theoretical work

Classroom/independent work: 0/30

E
-
learning: yes

Type of group:
groups of
2/3

7.

Teaching method: Theoretical study

Classroom/independent work: 0/45

E
-
learning: yes

Type of group: individual

8.

Teaching method: Evaluation

Classroom/independent work: 4/0

E
-
learning: no

Type of group: individual


Assessment instruments, criteria and learning agreement

Assessment criteria:

Knowledge of the role of microorganisms in the development of recombinant DNA
techniques, their influence on the development of biological concepts and their
biotechnological ap
plications. Acquisition of the practical foundations
underlying

the
various applications of recombinant DNA technology. Students must attend practical
sessions and pass the final exam to earn a passing grade in the course.

Assessment instruments:

Final exa
m

Group work presentations

Presentation of a report on practical work

Group work

Practical lab work

Supervised lab practicum

Lecture classes.

Grading criteria:

Final exam:

50%

Group work presentations:

15%

Presentation of a report on practical work:

5%

On
-
going assessment of group work:

10%

On
-
going assessment of practical lab work:

5%

On
-
going assessment of supervised lab practicum
:

10%

On
-
going assessment of lecture classes:

5%.

Assessment based on a learning agreement:

No


Independent study material and
recommended reading

GLICK, B.R.; PASTERNAK, J.J. (2003).
Molecular

Biotechnology. Principles and
A
pplications of Recombinant DNA
. Third ed. ASM Press. Washington
.


Bibliography, resources and annexes

OLD, PRIMROSE, S.B. (1994).
Principles of Gene Manipulat
ion

. Blackwell ed.

PERERA J., TORMOS A. & GARCÍA J.L. (2002).
Ingeniería
Genetics
. Vol. II. Ed.
Síntesis. Madrid
.

IZQUIERDO, M. (1999).
Ingeniería
genetics

y transferencia génica.

Ediciones
Pirámide, S.A. Madrid.

KENDREW, J. (1994).
The Encyclopedia of Mol
ecular Biology
. Blackwell Science.
Oxford.

KING, R.C.; STANSFIELD, W. D. (1997).
A Dictionary of Genetics
. Fifth edition.

Oxford University Press.

LUQUE, J.; HERRÁEZ, A. (2001).
Texto ilustrado de
Molecular biology

e Ingeniería
Genetics
. Conceptos, Técnica
s y Aplicaciones en Ciencias de la Salud.

Ed.
Harcourt, S.A.

NICHOLL, D.S.T. (2002).
An introduction to genetic engineering
. 2ª Ed. Cambridge
University Press. Cambridge.

PRIMROSE, S.B. (1998).
Principles of Genome Analysis
. Blackwell Science Ltd.


Link to

the course teaching guide