Course Outline

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BIOL 220
-
3 Introductory Microbiology II (3, 0, 2)

Course Outline Winter 2005


Instructors:

Lecture:


Naowarat Cheeptham

Office: S354


Section 01


Emai
l:
ncheeptham@cariboo.bc.ca



Phone: 371
-
5891


Office Hours: MWF 11
-
12 am, Th 10am


12 pm
; or make an appointment


Lab Instructor:

Joanna

Urban

Office: S35
2



Email:
jurban@cariboo.bc.ca



Phone: 371
-
5542


Lab Facu
lty:

Carolynne Fardy Office: S365G



Email
:
fardy@cariboo.bc.ca



Phone:
828
-
5444


Course Description:

BIOL 220
-
3 is a continuation of Introductory Microbiology 1, BIOL 210
-
3. The
course focuses on the vast array of applications in microbiology, which is often referred
to as microbial biotechnology. Lectures will cover the f
undamentals of bacterial genetics
and techniques for genetic engineering as well as the role of microbiology in medicine,
agriculture, and the environment. The theory, practice, and the importance of applied
microbiology in these areas are explored throug
h examples such as water and sewage
treatment. Laboratory sessions provide hands
-
on training in which the techniques covered
in lectures are applied. Experiments on screening for useful products of fermentation
technology and fundamental techniques in mic
robial genetics will be two topics used to
connect the importance of theory and practice in modern biotechnology.


Educational Objectives

BIOL 220
-
3 will introduce the students to a range of important roles that
microorganisms and their useful products pl
ay in modern biotechnology, food
technology, and medical, agricultural, environmental biotechnology. It will lead students
to a better understanding of the connection between the concepts and applications of
microorganisms, to better utilize and manage ou
r microbial communities.


Prerequisites:


In order to proceed to BIOL 220
-
3, a minimum of grade of C in BIOL 210
-
3 must be
achieved.



Course Structure

The course will consist of 3 hours of lectures and 2 hours of laboratory per week
(Note: The laboratory

does not always consist of a 2 hour block and may require work to
be completed outside of the scheduled laboratory period).

Lectures: MWF 10:00


10:50 in S373

Laboratory: Tuesday and Thursday Room S367


Texts/Materials


Main texts:


1.

BIOL 220 Laboratory

Manual available on WebCT.


2.

Madigan, M. T., Martinko, J. M., and Parker, J.
2003
. Brock: Biology of
Microorganisms: an International Edition 8
th

Edition. Prentice
-
Hall International, Inc.
Available in the bookstore. A few copies on reserve for in
-
library
use only (listed
under B
IOL

321).


Other resources in UCC library or on
-
line are:


1.

Holt, J. G.,
Krieg
, N. R.,
Peter H.A Sneath
,
Bergey
, D.
2000
.
Bergey's Manual of
Determinative

Bacteriology.

2.

Jensen, M. M., Wright, D. N., and Robinson, R. A.
1997
. Microbiology for the
Health Sciences: an International Edition 4
th

Edition. Prentice
-
Hall International,
Inc.

3.

Madigan, M. T., Martinko, J. M., and Parker, J.
1997
. Brock: Biology of
Mi
croorganisms: an International Edition 8
th

Edition. Prentice
-
Hall International,
Inc.

4.

Pelczar, M. J., Chan, E. C. S., and Krieg, N.R.
1993
. Microbiology: Concepts and
Applications. McGraw
-
Hill, Inc.

5.

Glazer, A. N.and Nikaido.
H.
1994
. Microbial Biotechnolo
gy: Fundamentals of
Applied Microbiology. W. H. Freeman and Company.

6.

Becker, G. M., Caldwell, G. A., and Zachgo E. A.
1990
. Biotechnology: A
laboratory course. Academic Press, Inc.

7.

Crueger, W. and Crueger, J. A.
1990
. Biotechnology: A Textbook of Industri
al
Microbiology, 2
nd

Edition. Sinauer Associates, Inc.


Assessment and schedule:


Lab quizzes, reports and subjective lab mark (evaluation of lab skills)



20%

Lab Final Exam (during final lab period)




10%

Review quiz


Friday, January 21
st

(based on Brock, Ch. 7)




5 %

Term test #1


Friday, February 11
th

(material from Jan. 10 to Feb. 4) 15%

Term test #2


Fr
iday, March 18
th

(material from Feb. 7 to Mar. 11)



15%

Assignment (
The Faces of Microbiology
, see detail in the attached assignment
)

5%


Final Exam (
ALL

material, scheduled by the Registrar)



30%



IMPORTANT:
In order to
PASS
the course you must complete all laboratory
assignments and lecture assignments. In addition, you must have a passing grade in both
the lecture component (term tests, final exam, and assignment) and the laboratory

component (lab quizzes and reports, subjective lab mark, lab final exam) to pass the
course. If you do not earn more than 50% in either the lecture component or the
laboratory component you will not earn the credit for the course.


If you are unable to be

present for the writing of any quiz or exam you must let Joanna or
I

know within 24 hours of when the test or exam is written. If you do not inform me of
your absence within the 24 hour time period, a zero grade will be given, except under
unusual circum
stances.


Lab quizzes will be administered every week during the laboratory session unless
otherwise stated. Lab quizzes will cover the content of the previous lab as well as the
introductory material and methodologies in the lab being performed that day.

The
average length of a lab quiz is ten minutes. At the end of the course, a final examination
for laboratory (in both theoretical and practical formats) will be given.


The lab and lecture quizzes may include the following question types.



Multiple choi
ce



Both/neither and matching



Fill in the blanks



Short answers


The midterm and final exams may include any of the above question types and in
addition include long answer questions.


Course Topics:

This is a list of topics that we are planning to cover i
n the course. The order in which
chapters are covered may change. You must read material in the text before coming to
class.


Brock
Chapter

Topic


7

Principles of microbial molecular biology

Self
-
study (review quiz on
Jan. 21)

8

Regulation of gene expres
sion


10

Bacterial genetics


15

Microbial genomics


31

Genetic engineering and biotechnology


30

Industrial microbiology


28

Wastewater treatment, water purification