BIOTECHNOLOGY - NARC

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23 Οκτ 2013 (πριν από 3 χρόνια και 5 μήνες)

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Nepal Agricultural Resear
ch Council

Syllabus for Scientist (S
-
1)

Open and internal competition examination

Sub Faculty


Biotechnology


1.

Tissue Culture:

a)

History

b)

Micropropagation

c)

Somatic embryogenesis

d)

Virus and pathogen elimination

e)

Haploid production by anther and ovary culture

f)

Embr
yo rescue

g)

Ploidy manipulation

h)

Germplasm conservation, storage and transportation (invitro, exsitu
conservation and cryopreservation)

i)

Production of secondary metabolites by cultured cells

j)

Callus culture

k)

Suspension culture

l)

Variability and instability in tiss
ue culture (Somaclonal variation)


2.

Molecular biology:

a)

Organization of plant genes, repetitive DNA

b)

Restriction enzymes

c)

Functional genes (Encoding RNA molecules, Proteins)

d)

Gene/s isolation and identification (cDNA, Genomic Library,
Transposons)

e)

Cloning state
gy (Plasmid, Cosmid, Bacteriophage etc.)

f)

Regulation of gene expression

g)

DNA methylation

h)

Antisense RNA

i)

Mutagenesis


3.

Cell biology:

a)

Isolation, culture and plant regeneration from protoplast

b)

Protoplast fusion

c)

Gene transfer into plant cells (Agrobacterium mediat
ed, direct gene
transfer)


4.

Methodologies and techniques:

a)

PCR techniques (RAPD, Microsatellite, AFLP)

b)

RFLP

c)

Agarose gel, starch gel and SDS
-
PAGE electrophoresis

d)

DNA sequencing

e)

Preparation of media, buffer and reagents for tissue culture and DNA
anlysis

f)

DNA
and isozyme extraction, isozyme staining

g)

Genetic analysis using computer software (MSTAT, GenSTAT, NTSys)

References:


1.

Bhojwani S and MK Razdan (1986). Plant tissue culture: Theory and practice.
Elsevier, Amsterdam.

2.

Reinert J and MM Yeoman (1982). Plant c
ell and tissue culture: a laboratory manual.
Berlin, Heidelberg and NewYork, Springer
-
Verlag.

3.

Kumar HD (1996). Molecular biology and biotechnology. Vikash Publishing House
Pvt. Ltd. New Delhi.

4.

Lindsey K and MGK Jones (1992). Plant biotechnology in agricult
ure. John Wiley
and Sons. Ltd., England.

5.

Maniatis T, E Fritsch and J Sambrook (1989). Molecular cloning: a laboratory
manual, Academy Press, New York.

6.

Old RW and SB Primorse (1985). Principles of gene manipulation: an introduction to
genetic engineering.

7.

N
eumann KH, W Barz and E Reinhard (1985). Primary and secondary metabolites of
plant cell culture. Heidelberg, Springer
-
Verlag

8.

Semal J (Ed.) (1986). Somaclonal variations and crop improvement. Dordrecht,
Nijhoff.

9.

Klee H, R Horsch and S Rogers (1987). Agrob
acterium mediated plant
transformation and its further application to biology. Ann. Rev. Plant Physiology 38,
pp 467
-
486.

10.

De Robertis EDP and EMF De Robertis (Jr.) (1995). Cell and molecular biology. BI
Waverly Pvt. Ltd., New Delhi.

11.

Meltzer SJ (1998). PCR
in bioanalysis. Human Press Inc. New Jersey

12.

Turner PC, AG McLennan, AD Bates and MRH White (1999). Instant notes in
molecular biology. Viva Books Pvt. Ltd., New Delhi.




Model Questions (Biotechnology, S
-
1)


Objective questions (20 questions), tick mark
the right answer:



20



1.

A cell with a haploid chromosome content (a) Aneuploid (b) Gamete (c) Ovule
(d) Gene


Define the following term (any five only)
:






10


1.

Physical map

2.

Plaque



Give short notes on any eight of the following
:





40


1.

Secondary metabolites

2.

Embryo rescue


Explain in detail:









20


Gene cloning


What do you understand by the following term (10 questions):



10


1.

Spectrophotometer
.