Biotechnology Study Guide false During replication, primase adds a ...

grapeafterthoughtΛογισμικό & κατασκευή λογ/κού

14 Δεκ 2013 (πριν από 3 χρόνια και 4 μέρες)

48 εμφανίσεις

Biotechnology Study Guide


1.

false

1.

During replication, primase adds a DNA primer to RNA.

2.

Recombinant DNA is the term used for

3.

Restrictive Enzyme

4.

List the 4 major areas of biotechnology.

2.

something that prevents or treats disease or alleviates the
symptoms of disease

1.

peptides

2.

medicine

3.

Genome

4.

Mole

3.

source of nutrients, in liquid or solid form, for growing cell cultures

1.

Variable

2.

Media

3.

Mole

4.

mitosis

4.

Vero cells

1.

These cells are cells of African green monkey kidneys.

2.

select the most appropriate ge
l concentraion

3.

Where does translation occur in prokaryotes?

4.

Which solution is more concentrated?

5.

an agent that stimulates the immune system ot provide protections against a particular
antigen or disease

1.

vaccine

2.

auxin

3.

Mole

4.

PAGE

6.

method of producing
identical organisms

1.

Cloning

2.

Cellulose

3.

Genome

4.

vaccine

7.

dominant

1.

When replacing inappropriate chromatography buffer with an appropriate one,
what volume of new buffer should you use for each round of a dialysis of 4 mL of
sample?

2.

How do DNA molecules va
ry from one species to another?

3.

If a plant inherits an allele for purple flowers from one parent and an allele for
yellow flowers from the other parent, and the flowers on this plant end up yellow,
then the yellow allele is

4.

List at least five of the most

common organisms used in biotechnology.

8.

X
-
Gluc and forms a blue product

1.

The enzyme encoded by the GUS gene breaks down

2.

cellulose molecules are

3.

Proteins are composed of

4.

What enzyme secures amino acids together by peptide bonds?

9.

USDA

1.

Separates larger
molecules such as restriction digest fragments

2.

What federal agency regulates the use and production of plants, plant pests,
veterinary supplies and medications and genetically modified plants and animals?

3.

Which of the following is an example of a product

easily found in nature?

4.

Several hundred patients are used to study safety, dosage, and efficacy of the
product.

10.

Phase III

1.

the part of a new plant that ruptures the seed coat during germination is called
the

2.

Sterilizations performed during the years in
which eugenics was practiced were

3.

Several thousand patients receive treatment with the pharmaceutical. In addition
to monitoring safety and efficacy, a comparison with other available treatments is also
done.

4.

What do plant cells need to survive in plant
tissue culture?

11.

enzyme that binds disconnected strands of DNA

1.

DNA Ligase

2.

vaccine

3.

PAGE

4.

mitosis

12.

stem

1.

Respiration

2.

germination

3.

water and food transportation

4.

Isolate gene of interest

13.

gel electrophoresis

1.

Plant cell walls can be ruptured

2.

The columns use
d in FPLC are

3.

The presence of plasmid DNA can be checked by

4.

pollen gathered by a bee

14.

the OH
-

concentration must be decreasing

1.

Determining the concentration of chlorophyll.

2.

If the H+ concentration in a sample is increasing

3.

add loading dye to samples

4.

A

solution that resists changes in pH.

15.

transmittance

1.

A replication fork is

2.

Amount of light that passes through a sample.

3.

Which of the following is a weak base?

4.

stain gel with Coomassie Blue

16.

the swelling of the explant

1.

In nature, the Ti plasmid

2.

The
region of activity on an enzyme is the

3.

Most of the DNA in humans is

4.

the first sign of a successful plant tissue culture is

17.

a mixture of two or more substances where one completely dissolves in the other

1.

Translation

2.

Solution

3.

Liter

4.

Mole

18.

haploid

1.

Multichannel Pipet

2.

peptides

3.

peptide synthesizer

4.

pollen gathered by a bee

19.

The PA approach (Practical Applications) is when you discuss short and long term results and
applications in a conclusion. The PE approach (Possible Errors) is where you discuss an
y of the
possible errors you might have found / came across while conducting the experiment and
how that would cause you to have false data. The REE approach (Results with Evidence and
Explanation) is where you state the evidence from your experiment with
numerical data. In
the REE approach you will say whether or not your data supports or refutes your hypothesis.

1.

Describe the REE, PE, PA approach to writing a conclusion to an experiment.

2.

What is the purpose of SDS during electrophoresis?

3.

Which of the fo
llowing is not a mass/volume concentration unit?

4.

What is the concentration of H+ in a solution with a pH of 3?

20.

describing a solution in which the solvent is water

1.

Aqueous

2.

Proteins

3.

Probe

4.

Media

21.

the liquid above a pellet after centrifugation

1.

Supernatant

2.

Proteins

3.

Pipet

4.

Base Pair

22.

they need stronger nails and hair

1.

what can be added to a plant cutting to encourage it to root?

2.

Which of the following is a strong acid?

3.

Biotechnology research labs are commonly found in all of the following facilities
except

4.

All of the following are reasons some people need recombinant protein
therapeutics except:

23.

either

1.

Gene Therapy

2.

Transformed

3.

Recombinant DNA

4.

Used to separate DNA samples

24.

class of lipids primarily found in cell membranes

1.

Phospholipids

2.

Mitochondr
ia

3.

Steroids

4.

Proteases

25.

Phase I

1.

What are HeLa cells and why are they important?

2.

A few high
-
risk patients are used to help determine drug safety, dosage, and
potential side effects.

3.

All of the following are reasons to use TRIS during an electrophoresis
except

4.

What is the best way to breed 1000 more plants of a rare orchid?

26.

a process of making an RNA code from DNA

1.

Fermentation

2.

Transcription

3.

Antibodies

4.

Cofactor

27.

using pollen from one plant to pollinate a flower on another plant

1.

Molarity

2.

Normality

3.

cross
-
breeding

4.

Phospholipids

28.

amino acids

1.

Exponential Growth

2.

Proteins are composed of

3.

transformation

4.

oligonucleotides

29.

stem cutting, cleared of leaves, containing 2 nodes and shoot tip intact

1.

which propagation method would probably yield the best resul
ts?

2.

Which sample would absorb the most light?

3.

Which food is an example of a plant ovary?

4.

When a regulatory molecule binds to an operator,

30.

2000

1.

A typical E. coli genome contains about _____ genes.

2.

The columns used in FPLC are

3.

Used for separating
proteins

4.

Which sample would absorb the most light?

31.

determining a chromatography protocol in R&D

1.

How is DNA spliced?

2.

Open
-
column chromatography is most useful for

3.

Molecular Biology

4.

Concentration

32.

radicle

1.

The type of ribonucleic acid that is translated
into proteins is

2.

All of these are units of concentration except

3.

The light wavelength that the human eye can see.

4.

the part of a new plant that ruptures the seed coat during germination is called
the

33.

probes used specifically to initiate the polymerase cha
in reaction

1.

Aqueous

2.

AMU

3.

Organic

4.

Primers

34.

hormones, regulation, movement, protection, transport, catalysis, structure, storage

1.

What does it mean to probe DNA?

2.

What are the eight functions of proteins?

3.

water and food transportation

4.

What is a neutral s
olution?

35.

40 g; 95.3 g; 40.3 g; 36.5 g

1.

What is the molecular weight of the following compounds? NaOh; MgCl2; MgO;
HCl

2.

What is the largest scale of producing transformed cells called?

3.

What federal agency funds and conducts biomedical research?

4.

What are
the six characteristics of life?

36.

plasmid

1.

An example of a DNA vector is a(n)

2.

Fermentation

3.

explants

4.

A pure sample of water has

37.

a concentrated form of a reagent that is often diluted to form a "working solution"

1.

Phospholipids

2.

transfection

3.

Stock Solutio
n

4.

Dilution

38.

paper chromatography

1.

A solvent is used to separate out proteins, deposited on a filter paper, based on
their size and solubility.

2.

countries or regions with poor soil quality can grow plants

3.

How should the concentration of a colorless sample
be determined?

4.

How can colorless proteins be monitored during electrophoresis?

39.

23

1.

After doing a plasmid preparation, the presence of DNA can be confirmed by

2.

A flower heterozygous for pink petals produced 100 seeds from a cross with
another heterozygous
pink flowered plant. The seeds have been collected and planted
by a research assistant and they are now flowering. Which of the following numbers
would be expected for the number of blue flowering (recessive) offspring plants?

3.

Which of the following struc
tures are found in prokaryotic cells?

4.

new gene combinations are produced during meiosis as a result of

40.

circular piece of DNA used in recombination technology

1.

Probe

2.

Gram

3.

Plasmid

4.

placebo

41.

the substance in a solution that is being dissolved

1.

Balance

2.

Liter

3.

Solute

4.

drug

42.

making large numbers of batches of similar compounds at the same time

1.

peptides

2.

meiosis

3.

Base Pair

4.

parallel synthesis

43.

UV light spec

1.

Determine the concentration of calf thymus DNA sample.

2.

Nucleotides are connected together in a DNA m
olecule by

3.

DNA strand replication begins with an RNA primer.

4.

Reverse Transcriptase

44.

base

1.

An aqueous solution that ionizes and yields OH
-
.

2.

fruit that ripens slowly

3.

food production and storage

4.

Calculate the mass of 17 moles of HCl.

45.

quaternary structure

1.

oligonucleotides

2.

Prokaryotic DNA is contained in

3.

Disulfide bonds between different polypeptide chains

4.

List the 4 major areas of biotechnology.

46.

in the nucleus

1.

Where does transcription occur in eukaryotic cells?

2.

Which of the following is a strong acid?

3.

How does a buffer work?

4.

What are the six characteristics of life?

47.

repeating units that make up polymers

1.

Volume

2.

Enzymes

3.

Morals

4.

Monomers

48.

water
-
based media

1.

A spinner flask is

2.

Hydroponically grown plants are grown in

3.

Hydrophilic

4.

During fermentation,

49.

an instrument usually used to measure volumes between .1 mL and 50 mL

1.

Codon

2.

Mass

3.

Pipet

4.

Media

50.

the bottom of the meniscus should be at the graduation

1.

When measuring liquids using a graduated cylinder or pipet

2.

When a regulatory molecule binds to an
operator,

3.

A solution that resists changes in pH.

4.

run molecular weight standards in one lane

51.

an immortal tumor cell fused with a specific white blood cell that will produce monoclonal
antibodies

1.

Hybridomas

2.

haploid

3.

inbreeding

4.

Hybridize

52.

the scaled
-
up pr
ocess by which cells are grown under optimal conditions for maximum cell
division and product fermentation

1.

Fermentation

2.

Genetics

3.

transfection

4.

screening

53.

all of the above

1.

What is the concentration of H+ in a solution with a pH of 3?

2.

What part of an
amino acid differs from one to the other?

3.

List three factors that affect enzyme activity.

4.

Reverse transcriptase generates a lot of errors. What affect does this have on the
newly synthesized HIV particles?

54.

recombinant DNA

1.

A solution that resists changes

in pH.

2.

Host cell type is considered for target gene.

3.

A piece of DNA that results from being spliced into a vector is called

4.

Transfer gene of interest into a host organism

55.

proteins that break down other proteins

1.

Proteases

2.

Operon

3.

Aqueous

4.

hydroponics

56.

when cells grow rapidly such that in each cell cycle, the cell count doubles

1.

Cytology

2.

Exponential Growth

3.

Cellulose

4.

Solvent

57.

study of molecules that are found in cells

1.

Molecular Biology

2.

extracellular

3.

Cloning

4.

placebo

58.

flower or cone

1.

elution

2.

fraction

3.

Dilution

4.

reporduction

59.

an entire set of an organism's genetic information

1.

Balance

2.

placebo

3.

ATP

4.

Genome

60.

segments of nucleic acid that are 50 nucleotides or less in length

1.

Micropipet

2.

Clinical Trials

3.

oligonucleotides

4.

Antibodies

61.

values that help one
decide between right and wrong

1.

Volume

2.

Morals

3.

Mass

4.

Monomers

62.

product marketing

1.

all of the following are goals of plant biotechnology researchers who modify
plants and plant DNA except:

2.

All of the following must be monitored in the quality control and
quality
assurance departments of a pharmaceutical company except:

3.

All of the following may be used to induce changes at a particular locus on a DNA
molecule except:

4.

All of the following are benefits of producing human pharmacueticals in plants
rather tha
n in bacteria except:

63.

mass spectrometer

1.

Which sample would absorb the most light?

2.

How should the concentration of a colorless sample be determined?

3.

run molecular weight standards in one lane

4.

How can the molecular mass of a protein be determined?

64.

the de
sired result of endonuclease activity for the purpose of rDNA production

1.

microarray

2.

Pipet

3.

Sticky Ends

4.

Monomers

65.

true

1.

When considering the structure of DNA, we would say that complementary
strands are antiparallel.

2.

What enabled the onset of recombinant

DNA technology?

3.

How should the concentration of a colorless sample be determined?

4.

Monitoring the enzyme activity of amylase with an indicator solution.

66.

gene expression is turned off

1.

especially large molecules must be separated

2.

Which solution is more
concentrated?

3.

What are common enzyme cofactors?

4.

When a regulatory molecule binds to an operator,

67.

a single cell that is formed when an egg and sperm combine

1.

Virus

2.

Genome

3.

zygote

4.

frit

68.

pharmaceutical

1.

The type of ribonucleic acid that is translated into
proteins is

2.

The order of amino acids on a polypeptide chain is encoded by

3.

What organelle has no membrane and is the site of protein synthesis?

4.

A new _____ is an example of a product that could have been derived from
genetic engineering.

69.

a small glass sl
ide or silicon chip with thousands of samples on it that can be used to assess
the presence of a DNA sequence related to the expression of certain proteins

1.

microarray

2.

Mass

3.

Probe

4.

Balance

70.

a scientific magazine or periodical of published experiments

1.

Starch

2.

dominant

3.

Journal

4.

Solute

71.

plant tissue

1.

In a mini
-
prep, how is DNA extracted in the final step?

2.

If transformed bacteria containing the Ti plasmid are incubated with growth
media and _____, transgenic plants will develop.

3.

If the H+ concentration
in a sample is increasing

4.

All of the following are true about meristematic cells except:

72.

assays 99.9% reactivity

1.

column chromatography is used for all of the following except:

2.

All of the following might be reasons a product would not be marketable excep
t:

3.

About how long does the clinical testing phase typically last?

4.

the first sign of a successful plant tissue culture is

73.

all of these

1.

Enhancer

2.

Genes code for

3.

Reagent

4.

Probe

74.

a solution with equal numbers of H+ and OH
-

1.

transformation

2.

Transcription

3.

What is a neutral solution?

4.

What is the plant model organism?

75.

an antibiotic resistance gene is added to the vector

1.

What is a form of selection used to identify transformed cells?

2.

As a solution becomes more dilute

3.

How is gene expression controlled in
prokaryotic cells?

4.

Which of the following structures are found in prokaryotic cells?

76.

root

1.

restriction enzyme

2.

Photosynthesis

3.

food storage and anchorage

4.

Organic

77.

at the ribosomes

1.

Which food is an example of a plant ovary?

2.

Which of the following is a st
rong acid?

3.

What are the eight functions of proteins?

4.

Where does translation occur in prokaryotes?

78.

relatively large molecules

1.

especially large molecules must be separated

2.

When a regulatory molecule binds to an operator,

3.

In gel filtration chromatography
, which molecules would be collected in the first
fraction?

4.

The wavelength at which a sample absorbs the most light.

79.

an instrument that produces short sections of DNA, up to a few hundred base pairs in length

1.

parallel synthesis

2.

DNA ligase

3.

recessive

4.

DNA synthesizer

80.

10 microliters of DNA solution

1.

pollen gathered by a bee

2.

Which of the following can be measured using a micropipet?

3.

Identify a molecule that can be improved / enhanced

4.

The columns used in FPLC are

81.

by freezing with liquid nitrogen

1.

transg
enic plants

2.

Plant cell walls can be ruptured

3.

Used in vertical gel boxes

4.

peptide synthesizer

82.

using a UV spec

1.

How should the concentration of a colorless sample be determined?

2.

Host cell type is considered for target gene.

3.

As a solution becomes more dil
ute

4.

Which of the following is a weak base?

83.

true

1.

When scaling up cell cultures, assays are done

2.

These cells are cells of African green monkey kidneys.

3.

A characteristic of aging cells is that their telomeres become shorter.

4.

Transfer gene of interest int
o a host organism

84.

each new DNA molecule has half of the old one

1.

In nature, the Ti plasmid

2.

Exponential Growth

3.

Semi
-
conservative replication means:

4.

Proteins are composed of

85.

molecule that contains carbon and produced by living things

1.

explants

2.

cGMP

3.

Organic

4.

Proteins

86.

denature the proteins and give them a negative charge

1.

what serves as a vector for plant transformations?

2.

What is the purpose of SDS during electrophoresis?

3.

What are common enzyme cofactors?

4.

Which way do proteins migrate during gel ele
ctrophoresis?

87.

transformed

1.

kanamycin added to the growth media and a callus forms.

2.

Calculate the mass of 17 moles of HCl.

3.

Used in vertical gel boxes

4.

Light with wavelengths between 200 and 350 nm.

88.

tertiary structure

1.

The columns used in FPLC are

2.

Polypeptide folding due to interactions between charged R groups

3.

Determining the concentration of chlorophyll.

4.

Prokaryotic DNA is contained in

89.

50%

1.

What are the six characteristics of life?

2.

What percent of the offspring will have wrinkled seeds? [Use
the following cross:
A plant that is homozygous recessive for wrinkled seeds is crossed with a plant that is
heterozygous dominant for smooth seeds.]

3.

countries or regions with poor soil quality can grow plants

4.

When measuring liquids using a graduated cyl
inder or pipet

90.

sprouting of a seed into a new plant

1.

screening

2.

mitosis

3.

Media

4.

germination

91.

macromolecules that include fats, waxes, steroids and oils

1.

Solution

2.

diploid

3.

zygote

4.

Lipids

92.

psychology

1.

Determining the concentration of melanin pigment.

2.

Biotech
nologists must have a minimal understanding of all except

3.

Determining the concentration of chlorophyll.

4.

When scaling up cell cultures, assays are done

93.

hybridomas

1.

Monoclonal antibodies are produced by

2.

Nucleic Acids

3.

DNA synthesizer

4.

pollen gathered by a

bee

94.

2
-
5 years

1.

About how long does the clinical testing phase typically last?

2.

what can be used as cuttings during plant propagation?

3.

rose with long lasting blooms

4.

An aqueous solution that ionizes and yields H+.

95.

both a and b are correct

1.

The
antiparallel nature of double stranded DNA means

2.

A solution that resists changes in pH.

3.

Which of the following is a weak base?

4.

As a solution becomes more dilute

96.

Research and development of a product is very costly and having exclusive rights to the
prod
uct for a set period of time helps secure profitability

1.

Which way do proteins migrate during gel electrophoresis?

2.

What is the purpose of SDS when harvesting intracellular proteins?

3.

Why is patent protection an important concern in biotechnology and
pharm
aceutical companies?

4.

Folding due to interactions between polar / nonpolar amino acids

97.

lysosome

1.

How is gene expression controlled in prokaryotic cells?

2.

What organelle breaks down food molecules, wastes and invading antigens?

3.

The antiparallel nature of
double stranded DNA means

4.

what serves as a vector for plant transformations?

98.

cytokinin

1.

The columns used in FPLC are

2.

Plant cell walls can be ruptured

3.

faster growing roots

4.

faster development of callus during plant tissue culture

99.

bursts cells open

1.

cytokinin

2.

lysozyme

3.

Probe

4.

biochip

100.

a unit of measurement for volume, approximately equal to one quart

1.

Control

2.

Mole

3.

Liter

4.

cloning

101.

.2351 mL

1.

Used in horizontal gel boxes

2.

rose with long lasting blooms

3.

The columns used in FPLC are

4.

Convert 235.1
microliters to mL. Would it be

102.

extracellular

1.

All of these are units of concentration except

2.

What does it mean to probe DNA?

3.

These cells are cells of African green monkey kidneys.

4.

_____ proteins are easier to isolate from cell cultures.

103.

a protein produc
t grown in plants for human medicinal purposes

1.

Clinical Trials

2.

plant
-
based pharmaceutical

3.

Supernatant

4.

Base Pair

104.

active site

1.

The enzyme encoded by the GUS gene breaks down

2.

The region of activity on an enzyme is the

3.

As a solution becomes more dilute

4.

A solution that resists changes in pH.

105.

type of plant hormone that directs cell elongation

1.

auxin

2.

frit

3.

Exon

4.

Sugar

106.

chemical used in an experiment

1.

meiosis

2.

Media

3.

Reagent

4.

Pipet

107.

medical clinics

1.

Biotechnology research labs are commonly found in all of the

following facilities
except

2.

What federal agency funds and conducts biomedical research?

3.

What part of an amino acid differs from one to the other?

4.

faster development of callus during plant tissue culture

108.

pore size

1.

Which sample is the most concentrated?

2.

cells are burst open to retrieve DNA

3.

Which of the following is a weak base?

4.

especially large molecules must be separated

109.

the synthesis of drug molecules in a laboratory from simpler, pre
-
existing molecules

1.

Recombinant DNA

2.

Proteins

3.

organic synthesis

4.

dominant

110.

a DNA molecule that is used as a host to carry foreign pieces of DNA into a cell

1.

Vector

2.

ATP

3.

Liter

4.

Weight

111.

sonication of cell culture

1.

Which of the following is a weak base?

2.

Which of the following methods may be used to recover intracellular
proteins?

3.

Which of the following is a strong acid?

4.

How many different amino acids are known to be in proteins?

112.

only when moving cells from spinner flasks into fermenters

1.

pH is a measurement of _____ in a sample.

2.

When scaling up cell cultures, assays ar
e done

3.

extracellular

4.

add loading dye to samples

113.

620 g

1.

tall variety of sunflowers

2.

What are common enzyme cofactors?

3.

cellulose molecules are

4.

Calculate the mass of 17 moles of HCl.

114.

visible light spec

1.

the most challenging method of asexual plant
propagation is

2.

The enzyme encoded by the GUS gene breaks down

3.

Monitoring the enzyme activity of amylase with an indicator solution.

4.

How do DNA molecules vary from one species to another?

115.

Francis Galton

1.

What organelle has no membrane and is the site of
protein synthesis?

2.

What is the purpose of conducting double
-
blind tests during clinical trials?

3.

What is the best way to breed 1000 more plants of a rare orchid?

4.

Who was the scientist that coined the name eugenics for the theory of social
change was

116.

colu
mn chromatography

1.

antibody
-
antigen interactions are the basis of this method. As a sample flows
through the antibody
-
coupled resin, specific epitopes on the protein of interest will
bind to the antibodies.

2.

What is the concentration of H+ in a solution wi
th a pH of 3?

3.

Folding due to interactions between polar / nonpolar amino acids

4.

Amount of light that passes through a sample.

117.

0.4 mL

1.

When propagating plants by tissue culture, what type of tissue is used?

2.

What is the concentration of H+ in a solution wi
th a pH of 3?

3.

300 mL of a 1/40 dilution of 20 xTBE buffer stock solution is needed, what
volume of solute is needed?

4.

All of the following regulate gene expression in eukaryotes except:

118.

a specific region on an antigen where an antibody will bind

1.

Virus

2.

C
ofactor

3.

Steroids

4.

cloning

119.

Wash the vessel with laboratory soap and water. Rinse with tap water until no
evidence of soap remains. Rinse five+ times with tap water. Do a final rinse with deionized
water.

1.

All of the following should be included in a legal
scientific notebook except:

2.

Checking for the presence of an unknown, colorless protein.

3.

Disulfide bonds between different polypeptide chains

4.

Describe how glassware should be prepared before using it to prepare or store
solutions.

120.

a factor that gets chan
ged during an experiment

1.

explants

2.

Virus

3.

Variable

4.

Organic

121.

200
-
350

1.

Light with wavelengths between 200 and 350 nm.

2.

how do woody plants increase in width?

3.

What is a neutral solution?

4.

What range of wavelength is used by UV specs?

122.

macromolecules that dir
ect synthesis of cellular molecules

1.

screening

2.

sonication

3.

Nucleic Acids

4.

Cofactor

123.

a specialized type of B
-
cell that remains in the body for long periods of time with the
ability to make antibodies to a specific antigen

1.

B
-
cells

2.

Hormones

3.

Micropipet

4.

memory cell

124.

a specific kind of antibody made by hybridomas to target a particular epitope

1.

Recombinant DNA

2.

transformation

3.

Mitochondria

4.

Monoclonal Antibody

125.

visible light spec

1.

If the H+ concentration in a sample is increasing

2.

Determining the concentrati
on of melanin pigment.

3.

List the 4 major areas of biotechnology.

4.

Eukaryotic gene expression can be increased by

126.

specialized cells of the immune system that are used to generate and release
antibodies

1.

zygote

2.

dominant

3.

Steroids

4.

B
-
cells

127.

Alu I

1.

Which restr
iction enzyme listed in the chart below will result in a blunt end cut?

2.

Which of the following can be measured using a micropipet?

3.

What measurement does the detector in a spec make?

4.

A pair of alleles for a particular trait is called

128.

using a cell lysis
solution

1.

cells are burst open to retrieve DNA

2.

Used in horizontal gel boxes

3.

Concentration

4.

During fermentation,

129.

four polypeptides made up that protein

1.

Transfer gene of interest into a host organism

2.

A single protein was denatured and electrophoresed.
After visualization, four
bands were noticed. Two were small and two were large. What can you infer from
these results?

3.

A piece of DNA that results from being spliced into a vector is called

4.

Separates larger molecules such as restriction digest fragments

130.

all are units of concentration

1.

Fermentation

2.

All of these are units of concentration except

3.

A solution that resists changes in pH.

4.

Determining the concentration of chlorophyll.

131.

a molecule on which an enzyme acts

1.

Carbohydrates

2.

Substrate

3.

Solute

4.

Base
Pair

132.

plasma membrane

1.

Which of the following is the correct order of organization
-

smallest to largest?

2.

Which food is an example of a plant seed?

3.

Which of the following is a strong acid?

4.

Which of the following structures are found in prokaryotic cells?

133.

cells, tissue, organ, organ system, organism

1.

Which of the following is the correct order of organization
-

smallest to largest?

2.

Which food is an example of a plant ovary?

3.

Which of the following is an example of sterile technique?

4.

Which of the
following is a weak base?

134.

in eukaryotic cells to remove introns

1.

In a mini
-
prep, how is DNA extracted in the final step?

2.

What suffix is generally given to enzymes?

3.

What measurement does the detector in a spec make?

4.

Under what conditions do post transcri
ptional modifications to mRNA occur?

135.

the origin and development of a disease

1.

Cytology

2.

Proteases

3.

Photosynthesis

4.

pathogenesis

136.

350 nm

1.

At what wavelength would a red molecule absorb the most light?

2.

What enabled the onset of recombinant DNA technology?

3.

W
hat do plant cells need to survive in plant tissue culture?

4.

What range of wavelength is used by visible light specs?

137.

heat shock in warm water bath followed by ice bath

1.

What organelle breaks down food molecules, wastes and invading antigens?

2.

What enzyme
secures amino acids together by peptide bonds?

3.

What treatment encourages cells to incorporate rDNA plasmids during a bacterial
transformation?

4.

What is the purpose of conducting double
-
blind tests during clinical trials?

138.

the amount of a substance in propo
rtion to another substance

1.

elution

2.

Intron

3.

Concentration

4.

Stock Solution

139.

meters

1.

Volume is measured in all these units except

2.

Used to separate DNA samples

3.

pH is a measurement of _____ in a sample.

4.

Calculate the mass of 17 moles of HCl.

140.

40 mL

1.

How can
antibodies recognize different epitopes?

2.

Identify a molecule that can be improved / enhanced

3.

When replacing inappropriate chromatography buffer with an appropriate one,
what volume of new buffer should you use for each round of a dialysis of 4 mL of
samp
le?

4.

When scaling up cell cultures, assays are done

141.

process by which plants use light energy to make chemical energy

1.

Photosynthesis

2.

Antibodies

3.

peptides

4.

Steroids

142.

hormones

1.

Genes that control the production of _____ trigger cell specialization during
seed
germination.

2.

Harvest desired product from cell culture

3.

Thin layer chromatography is useful for separating

4.

What is the purpose of SDS when harvesting intracellular proteins?

143.

primary structure

1.

Most of the DNA in humans is

2.

An example of a DNA vector

is a(n)

3.

A pure sample of water has

4.

The order of amino acids in a polypeptide chain

144.

pH balance, temperature, concentration

1.

The region of activity on an enzyme is the

2.

The light wavelength that the human eye can see.

3.

Which of the following is a strong
acid?

4.

List three factors that affect enzyme activity.

145.

nucleotide that serves as energy storage molecule

1.

Gram

2.

ATP

3.

Vector

4.

Exon

146.

cloned

1.

after a plant is transformed with human DNA, it must be _____ and then planted
in the field.

2.

What is the most
convenient way to determine the pH of a single sample?

3.

countries or regions with poor soil quality can grow plants

4.

What is the concentration of H+ in a solution with a pH of 3?

147.

1.4 au

1.

Which solution is more concentrated?

2.

Which sample is the most concen
trated?

3.

What is the plant model organism?

4.

Why are plasmid preparations done?

148.

operons

1.

How is gene expression controlled in prokaryotic cells?

2.

Where does transcription occur in eukaryotic cells?

3.

How can antibodies recognize different epitopes?

4.

What are

the eight functions of proteins?

149.

not transformed

1.

faster development of callus during plant tissue culture

2.

x
-
gluc added to the growth media and plants are a vibrant green

3.

Convert 1.7 L to mL. Would it be

4.

food storage and anchorage

150.

negatively charged
beads

1.

If a positively charged molecule is to be separated by ion exchange
chromatography, what sort of resin should be used?

2.

If you want to separate 75 kD and 300 kD molecules from each other by gel
filtration chromatography, which pore size would yield
the best result?

3.

Host cell type is considered for target gene.

4.

How might the fractions containing colorless target proteins be visualized after
chromatography?