Activity 3: Characterization and Simulation: Cell Environment

foulgirlsΠολεοδομικά Έργα

15 Νοε 2013 (πριν από 3 χρόνια και 9 μήνες)

85 εμφανίσεις

Multidisciplinary Team Building Grant

28 March 2012

Guan et al.
1

Activity 3: Characterization and Simulation:

Cell Environment

Peter M. Anderson, Materials Science

Heather Powell, Materials Science/Biomedical Engineering

Samir Ghadialli, Biomedical Engineering

Gregory Lafyatis, Physics


Supported Graduate Student:

Yanyi Xu



Support: OSU Materials Research Institute

Multidisciplinary Team Building Grant

28 March 2012

Guan et al.
2

Activity 3: Goal


Quantify



the 3D mechanical environment experienced by cells


Approach


accurately characterize fibrous matrix geometry


inform finite element models with


fiber properties


matrix geometry


matrix macromechanical response


use FE model to determine


local mechanical environment (Green's function)


cell force footprints


correlate environment and differentiation

cyclic excitation

Multidisciplinary Team Building Grant

28 March 2012

Guan et al.
3

Activity 3: FE Simulations


Relates Structure and Properties

matrix

geometry

mechanical

re獰潮獥㨠浡瑲楸

䙩F楴e 䕬E浥n琠
䵯Mel


浥捨慮楣慬a
properties: fibers

cell force
footprint

energy
partitioning

Agarwal (AFMic)

Powell/Ghadiali (OM)

Lafyatis (DeCo)

Ghadiali (SiWa)

Powell (UniT)

Anderson (NInd)

Multidisciplinary Team Building Grant

28 March 2012

Guan et al.
4

Activity 3: Matrix Geometry


Confocal Fluorescence
Microscopy


heavy die loading/imaging
(Powell/Ghadiali)


deconvolution (Lafyatis)


conversion to finite element
data file (Ghadiali/Anderson)

Multidisciplinary Team Building Grant

28 March 2012

Guan et al.
5

Activity 3: Preliminary Simulations


ABAQUS software


54 fibers: beam elements


20 x 20 x 5
m
洠syst敭


110% axial strain


imposed in X direction.


Negative transverse
strain


Possion effect


X

Y

Z

Simu

Scaffold Simulations

Multidisciplinary Team Building Grant

28 March 2012

Guan et al.
6

Activity 3: Preliminary Simulations