Materials Core Instrumentation:
1.
UV
-
Vis
-
NIR spectrophotometer DU 640B (Beckman)
2.
FTIR spectrophotometer FTIR
-
480 (Jasco)
3.
Spectropolarimeter
J
-
810 (
Jasco
)
4.
Refractive index analysis instrument AR700 (Reichert)
5.
CAM 200 optical contact
-
angle
goniometer
(KSV Instruments)
6.
Ellipsometer
GES 5, variable
-
angle spectroscopic (
Sopra
Inc)
7.
Flame atomic absorption
-
AAnalyst
200 (Perkin Elmer)
8.
SPR spectrometer
Biacore
X
9.
MTS
Synergie
100
10.
HPLC/GPC system (Waters)
11.
Plasma surface treatment unit (Harrick)
More details on each instrument are on the following slides.
1
For more info, please contact Dr.
Gulya
Korneva
:
gkornev@clemson.edu
Spectrophotometer DU 640B, Beckman
Applications:
Measures
Absorbance and
Transmission:
1)
At fixed wavelength;
2)
Wavelength scan;
3)
Kinetics/time
Can be used in qualitative and
quantitative analyses.
2
Wavelength range 190
-
1100 nm
Fourier Transform Infrared
Spectrophotometer FTIR
-
480, Jasco
Applications:
•
structure analysis;
•
purity examinations;
•
quantitative analysis;
•
identification of unknown compounds:
liquids, semi
-
liquids and flexible solids;
•
biological materials study;
with HATR plates
ZnSe
highly absorbing
substances can be analyzed (polymers,
rubber, fibers, etc);
Specifications:
Wavelength range: 360 to
7800 cm
-
1
;
•
Resolution range: 0.5;0.9;2;4;8;16 cm
-
1
3
Accessories
:
1)
MIRacle
TM
single reflection HATR (
horisontal
ATR, Pike technologies) with 2
mm round crystal
ZnSe
. 2) &3) HATR plates in “through” and “flat” configurations. “
Through”
–
for liquid, powder, or paste, good for volatile compounds;
“flat”
–
for the analysis of solid
materials including polymer and film samples.
For more info:
www.
jasco
inc.com/Libraries/Application_Notes/IR_01_03.sflb.ashx
Spectropolarimeter J
-
810, Jasco
Specifications
Light source: 150W
Xe
arc lamp (N2 cooled)
Wavelength
range: 163 ~ 900
nm
Accessories
:
Peltier
type temperature controller
Jasco PFD
-
425S
Automated pH
titrator
Jasco ATS 429S
Measures the circular
dichroism
(CD)
and optical rotary dispersion (ORD)
of the sample.
Applications:
•
Allows to verify the
chirality
of
molecules or compounds;
•
to estimate fractions of each
different conformation in protein
structure;
•
the conformational changes of
molecules with temperature/and pH
can be studied.
Useful for verifying that the protein
is in its native conformation.
•
Protein folding studies.
•
Purity testing of optically active
substances.
4
Refractive index analysis instrument
–
Reichert AR700
For more info:
http
://
www.reichertai.com/products.html?productID=17
Temperature Controlled
Automatic
Refractometer
Applications:
Measures refractive index of
transparent, translucent, and
opaque liquids.
Features
:
Reading range of 1.33000 to
1.60000 Refractive Index and the
full
Brix
scale.
Precision: to 0.01
Brix
or
0.00001
5
CAM 200 (KSV)
CAM 200 is a video
camera based fully
computer controlled
contact angle meter.
Applications:
•
Contact angle
measurements;
•
Surface free energy
measurements;
For more info:
http://www.terralab.biz/edoc/ksv/KSV_Cam_200_Contact_Angle_Surface_Tension_Meter.pdf
CCD Camera
•
Absorption and wetting;
•
Surface/interfacial tension
6
Variable
-
angle spectroscopic
ellipsometer
GES 5 (
Sopra
Inc)
Spectroscopic
ellipsometry
(SE)
is a non
-
contact, non
-
destructive
optical technique.
Applications:
•
measures material refractive
indices;
•
layer thicknesses of thin film
layers on flat substrates;
•
up to 7 layers can be analyzed
in terms of thickness and optical
characteristics for each layer.
Features:
Thickness measured: from
monoatomic
to several microns thick. The beam size: 1 mm
2
and 10 mm
2
;
The variable angles: from 7º to 90º with variable +/
-
0.01º;
spectral range from 200 nm to 800 nm
7
For more info:
http://www.sopra
-
sa.com/ges5e
-
for
-
retd
-
pdt
-
5
-
16.php
Flame atomic absorption spectrometer
-
AAnalyst
200, Perkin Elmer
Applications:
•
for determining very precisely the
concentration of a particular element in a
liquid sample. Can detect from 1 to 1000
µg/L.
For each element, the specific lamp is
required. Currently, Ca lamp is available.
The list of lamps/elements for analysis on
AAnalyst
200 can be found on
https://www.vwrcanlab.com/programs/equipment/
pdf/Model%20AAnalyst%20200%20Spectrometer
s.pdf
8
Biacore
is a methodology for measuring the
biomolecular
interactions in real time using
the phenomenon of
surface Plasmon
resonance (SPR).
Applications:
it allows to study:
•
Kinetics
of binding
• Solution affinity
• Steady state affinity
• Yes/No binding
•
Ligand
fishing
• Affinity ranking
• Protein concentration assays
• Protein percent activity assays
• Thermodynamics
Interactions between
nucleic
acids, lipids,
small
molecules, whole cells, and surfaces
can be studied.
BiAcoreX
SPR spectrometer
Specifications:
Two
Flow cell capability
Temperature regulation = 4
-
40
o
C
Flow rates
1
-
100μL/min
, steps of 1
μL
Required
sample volume: injection
volume
(min
-
5
μL
+
20
μl
)
Molecular weight detection: >180
Da
Refractive index range: 1.33
-
1.40
Sensitivity ≈ 20 RU, where
RU, resonance
units,
1RU
=
1
pg/mm
2
of sensor surface
.
9
Some applications are described in a paper by Y. Wei
and R. A. Latour,
Langmuir
2009,
25(10), 5637
–
5646
For more info:
http://web.bf.uni
-
lj.si/bi/sprcenter/BiacoreX.pdf
Materials Testing System (MTS)
Synergie
100
Testing:
Tensile
, Flex, Peel/Tear, Strain, and Compression test
methods.
1) Loadings available: 10 N (2
lbf
)
2) Speed
range: 0.001
-
1000 mm/min (0.00004
-
39 in/min)
3) Max
specimen thickness
for grips: 13
mm (0.5 in
)
4) 50 mm (2.0 inch) diameter lightweight compression
platens
Face
set, 25x25 mm rubber, for 100/2000 advantage
pneumatic and screw action
grips.
For more info:
http://www.mts.com/en/Bio/Materials/index.asp
10
HPLC/GPC system (Waters)
Applications:
high resolution
separations and purifications
Includes:
I.
Binary Gradient Pumping
System (1525)
I.
Autosampler
(2707)
II.
Photodiode Array Detector (2998)
III.
Refractive Index Detector (2414)
IV.
Column Heater
V.
Fraction Collector
Specifications:
I.
Binary
Gradient Pumping
System: Flow Rate
Range:
0
to 10
ml/min; max
operating
p
ressure
: 6000
psi.
II.
Autosampler
: Loop Volumes: 100 and 500 µl.
III.
Refractive Index Detector: Refractive index range: 1.00 to 1.75 RIU.
IV.
Column Heater: Temperature range: 20
o
C to 60
o
C; column capacity: up to
four 7.8 mm x 300 mm columns
For more info:
http://www.waters.com/waters/nav.htm?locale=en_US&cid=514256
11
Plasma cleaner/sterilizer, Harrick
For more info:
http://harrickplasma.com/applications_biomaterials.php
Applications:
•
Plasma activation of substrate surfaces to
render surface hydrophilic; promote attachment
and adhesion of functional biological species or
coatings to:
1)
Enhance cell adhesion, coverage, and
proliferation on tissue scaffolds.
2)
Promote adsorption of selective functional
biological species while resisting, adhesion
of bacteria and fouling microorganisms.
•
Removes organic contamination from surfaces.
•
Modifies or enhances the physical and chemical characteristics of surfaces: The
high energy plasma particles combine with the contaminant to form carbon dioxide
(CO
2
) or methane (CH
4
).
12
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