Materials Core Instrumentation:

Materials Core Instrumentation:

1.
UV
-
Vis
-
NIR spectrophotometer DU 640B (Beckman)

2.
FTIR spectrophotometer FTIR
-
480 (Jasco)

3.
Spectropolarimeter

J
-
810 (
Jasco
)

4.
Refractive index analysis instrument AR700 (Reichert)

5.
CAM 200 optical contact
-
angle
goniometer

(KSV Instruments)

6.
Ellipsometer

GES 5, variable
-
angle spectroscopic (
Sopra

Inc)

7.
Flame atomic absorption
-

AAnalyst

200 (Perkin Elmer)

8.
SPR spectrometer
Biacore

X

9.
MTS
Synergie

100

10.
HPLC/GPC system (Waters)

11.
Plasma surface treatment unit (Harrick)

More details on each instrument are on the following slides.

1

For more info, please contact Dr.
Gulya

Korneva
:
gkornev@clemson.edu


Spectrophotometer DU 640B, Beckman

Applications:

Measures
Absorbance and
Transmission:

1)
At fixed wavelength;

2)
Wavelength scan;

3)
Kinetics/time

Can be used in qualitative and
quantitative analyses.

2

Wavelength range 190
-
1100 nm


Fourier Transform Infrared
Spectrophotometer FTIR
-
480, Jasco

Applications:

•

structure analysis;

•

purity examinations;

•

quantitative analysis;

•

identification of unknown compounds:
liquids, semi
-
liquids and flexible solids;

•

biological materials study;

with HATR plates
ZnSe

highly absorbing
substances can be analyzed (polymers,
rubber, fibers, etc);

Specifications:

Wavelength range: 360 to
7800 cm
-
1
;

•

Resolution range: 0.5;0.9;2;4;8;16 cm
-
1

3

Accessories
:
1)
MIRacle
TM

single reflection HATR (
horisontal

ATR, Pike technologies) with 2
mm round crystal
ZnSe
. 2) &3) HATR plates in “through” and “flat” configurations. “
Through”

–

for liquid, powder, or paste, good for volatile compounds;

“flat”
–

for the analysis of solid
materials including polymer and film samples.


For more info:
www.
jasco
inc.com/Libraries/Application_Notes/IR_01_03.sflb.ashx



Spectropolarimeter J
-
810, Jasco

Specifications

Light source: 150W
Xe

arc lamp (N2 cooled)

Wavelength
range: 163 ~ 900
nm

Accessories
:



Peltier

type temperature controller
Jasco PFD
-
425S



Automated pH
titrator

Jasco ATS 429S


Measures the circular
dichroism

(CD)
and optical rotary dispersion (ORD)
of the sample.

Applications:

•

Allows to verify the
chirality

of
molecules or compounds;

•

to estimate fractions of each
different conformation in protein
structure;

•

the conformational changes of
molecules with temperature/and pH
can be studied.

Useful for verifying that the protein
is in its native conformation.

•

Protein folding studies.

•

Purity testing of optically active
substances.

4

Refractive index analysis instrument
–

Reichert AR700

For more info:
http
://
www.reichertai.com/products.html?productID=17



Temperature Controlled
Automatic
Refractometer


Applications:

Measures refractive index of
transparent, translucent, and
opaque liquids.


Features
:

Reading range of 1.33000 to
1.60000 Refractive Index and the
full
Brix

scale.

Precision: to 0.01
Brix

or
0.00001

5

CAM 200 (KSV)

CAM 200 is a video
camera based fully
computer controlled
contact angle meter.


Applications:

•

Contact angle
measurements;

•

Surface free energy
measurements;

For more info:
http://www.terralab.biz/edoc/ksv/KSV_Cam_200_Contact_Angle_Surface_Tension_Meter.pdf


CCD Camera

•

Absorption and wetting;

•

Surface/interfacial tension


6

Variable
-
angle spectroscopic
ellipsometer

GES 5 (
Sopra

Inc)

Spectroscopic
ellipsometry

(SE)
is a non
-
contact, non
-
destructive
optical technique.

Applications:

•

measures material refractive
indices;

•

layer thicknesses of thin film
layers on flat substrates;

•

up to 7 layers can be analyzed
in terms of thickness and optical
characteristics for each layer.

Features:

Thickness measured: from
monoatomic

to several microns thick. The beam size: 1 mm
2

and 10 mm
2
;

The variable angles: from 7º to 90º with variable +/
-

0.01º;

spectral range from 200 nm to 800 nm


7


For more info:
http://www.sopra
-
sa.com/ges5e
-
for
-
retd
-
pdt
-
5
-
16.php


Flame atomic absorption spectrometer
-

AAnalyst

200, Perkin Elmer

Applications:

•

for determining very precisely the
concentration of a particular element in a
liquid sample. Can detect from 1 to 1000
µg/L.

For each element, the specific lamp is
required. Currently, Ca lamp is available.

The list of lamps/elements for analysis on
AAnalyst

200 can be found on
https://www.vwrcanlab.com/programs/equipment/
pdf/Model%20AAnalyst%20200%20Spectrometer
s.pdf



8

Biacore

is a methodology for measuring the
biomolecular

interactions in real time using
the phenomenon of
surface Plasmon
resonance (SPR).

Applications:
it allows to study:

•
Kinetics
of binding

• Solution affinity

• Steady state affinity

• Yes/No binding

•
Ligand

fishing

• Affinity ranking

• Protein concentration assays

• Protein percent activity assays

• Thermodynamics

Interactions between
nucleic
acids, lipids,
small
molecules, whole cells, and surfaces

can be studied.


BiAcoreX

SPR spectrometer

Specifications:


Two
Flow cell capability


Temperature regulation = 4
-
40
o
C


Flow rates
1
-
100μL/min
, steps of 1
μL


Required
sample volume: injection
volume
(min
-
5
μL

+
20
μl
)


Molecular weight detection: >180
Da


Refractive index range: 1.33
-

1.40



Sensitivity ≈ 20 RU, where
RU, resonance
units,
1RU
=
1
pg/mm
2

of sensor surface
.

9

Some applications are described in a paper by Y. Wei
and R. A. Latour,
Langmuir
2009,
25(10), 5637
–
5646

For more info:
http://web.bf.uni
-
lj.si/bi/sprcenter/BiacoreX.pdf


Materials Testing System (MTS)
Synergie

100

Testing:

Tensile
, Flex, Peel/Tear, Strain, and Compression test
methods.

1) Loadings available: 10 N (2
lbf
)

2) Speed
range: 0.001
-
1000 mm/min (0.00004
-
39 in/min)

3) Max
specimen thickness

for grips: 13
mm (0.5 in
)

4) 50 mm (2.0 inch) diameter lightweight compression
platens

Face
set, 25x25 mm rubber, for 100/2000 advantage
pneumatic and screw action
grips.


For more info:
http://www.mts.com/en/Bio/Materials/index.asp


10

HPLC/GPC system (Waters)

Applications:
high resolution

separations and purifications

Includes:

I.
Binary Gradient Pumping


System (1525)

I.
Autosampler

(2707)

II.
Photodiode Array Detector (2998)

III.
Refractive Index Detector (2414)

IV.
Column Heater

V.
Fraction Collector


Specifications:

I.
Binary
Gradient Pumping
System: Flow Rate
Range:
0
to 10
ml/min; max
operating
p
ressure
: 6000
psi.

II.
Autosampler
: Loop Volumes: 100 and 500 µl.

III.
Refractive Index Detector: Refractive index range: 1.00 to 1.75 RIU.

IV.
Column Heater: Temperature range: 20
o
C to 60
o
C; column capacity: up to
four 7.8 mm x 300 mm columns






For more info:
http://www.waters.com/waters/nav.htm?locale=en_US&cid=514256

11

Plasma cleaner/sterilizer, Harrick

For more info:
http://harrickplasma.com/applications_biomaterials.php


Applications:

•

Plasma activation of substrate surfaces to
render surface hydrophilic; promote attachment
and adhesion of functional biological species or
coatings to:

1)
Enhance cell adhesion, coverage, and
proliferation on tissue scaffolds.

2)
Promote adsorption of selective functional
biological species while resisting, adhesion
of bacteria and fouling microorganisms.

•

Removes organic contamination from surfaces.

•

Modifies or enhances the physical and chemical characteristics of surfaces: The
high energy plasma particles combine with the contaminant to form carbon dioxide
(CO
2
) or methane (CH
4
).

12