Materials Core Instrumentation:

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27 Οκτ 2013 (πριν από 3 χρόνια και 5 μήνες)

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Materials Core Instrumentation:

1.
UV
-
Vis
-
NIR spectrophotometer DU 640B (Beckman)

2.
FTIR spectrophotometer FTIR
-
480 (Jasco)

3.
Spectropolarimeter

J
-
810 (
Jasco
)

4.
Refractive index analysis instrument AR700 (Reichert)

5.
CAM 200 optical contact
-
angle
goniometer

(KSV Instruments)

6.
Ellipsometer

GES 5, variable
-
angle spectroscopic (
Sopra

Inc)

7.
Flame atomic absorption
-

AAnalyst

200 (Perkin Elmer)

8.
SPR spectrometer
Biacore

X

9.
MTS
Synergie

100

10.
HPLC/GPC system (Waters)

11.
Plasma surface treatment unit (Harrick)

More details on each instrument are on the following slides.

1

For more info, please contact Dr.
Gulya

Korneva
:
gkornev@clemson.edu


Spectrophotometer DU 640B, Beckman

Applications:

Measures
Absorbance and
Transmission:

1)
At fixed wavelength;

2)
Wavelength scan;

3)
Kinetics/time

Can be used in qualitative and
quantitative analyses.

2

Wavelength range 190
-
1100 nm


Fourier Transform Infrared
Spectrophotometer FTIR
-
480, Jasco

Applications:



structure analysis;



purity examinations;



quantitative analysis;



identification of unknown compounds:
liquids, semi
-
liquids and flexible solids;



biological materials study;

with HATR plates
ZnSe

highly absorbing
substances can be analyzed (polymers,
rubber, fibers, etc);

Specifications:

Wavelength range: 360 to
7800 cm
-
1
;



Resolution range: 0.5;0.9;2;4;8;16 cm
-
1

3

Accessories
:
1)
MIRacle
TM

single reflection HATR (
horisontal

ATR, Pike technologies) with 2
mm round crystal
ZnSe
. 2) &3) HATR plates in “through” and “flat” configurations. “
Through”



for liquid, powder, or paste, good for volatile compounds;

“flat”


for the analysis of solid
materials including polymer and film samples.


For more info:
www.
jasco
inc.com/Libraries/Application_Notes/IR_01_03.sflb.ashx



Spectropolarimeter J
-
810, Jasco

Specifications

Light source: 150W
Xe

arc lamp (N2 cooled)

Wavelength
range: 163 ~ 900
nm

Accessories
:



Peltier

type temperature controller
Jasco PFD
-
425S



Automated pH
titrator

Jasco ATS 429S


Measures the circular
dichroism

(CD)
and optical rotary dispersion (ORD)
of the sample.

Applications:



Allows to verify the
chirality

of
molecules or compounds;



to estimate fractions of each
different conformation in protein
structure;



the conformational changes of
molecules with temperature/and pH
can be studied.

Useful for verifying that the protein
is in its native conformation.



Protein folding studies.



Purity testing of optically active
substances.

4

Refractive index analysis instrument


Reichert AR700

For more info:
http
://
www.reichertai.com/products.html?productID=17



Temperature Controlled
Automatic
Refractometer


Applications:

Measures refractive index of
transparent, translucent, and
opaque liquids.


Features
:

Reading range of 1.33000 to
1.60000 Refractive Index and the
full
Brix

scale.

Precision: to 0.01
Brix

or
0.00001

5

CAM 200 (KSV)

CAM 200 is a video
camera based fully
computer controlled
contact angle meter.


Applications:



Contact angle
measurements;



Surface free energy
measurements;

For more info:
http://www.terralab.biz/edoc/ksv/KSV_Cam_200_Contact_Angle_Surface_Tension_Meter.pdf


CCD Camera



Absorption and wetting;



Surface/interfacial tension


6

Variable
-
angle spectroscopic
ellipsometer

GES 5 (
Sopra

Inc)

Spectroscopic
ellipsometry

(SE)
is a non
-
contact, non
-
destructive
optical technique.

Applications:



measures material refractive
indices;



layer thicknesses of thin film
layers on flat substrates;



up to 7 layers can be analyzed
in terms of thickness and optical
characteristics for each layer.

Features:

Thickness measured: from
monoatomic

to several microns thick. The beam size: 1 mm
2

and 10 mm
2
;

The variable angles: from 7º to 90º with variable +/
-

0.01º;

spectral range from 200 nm to 800 nm


7


For more info:
http://www.sopra
-
sa.com/ges5e
-
for
-
retd
-
pdt
-
5
-
16.php


Flame atomic absorption spectrometer
-

AAnalyst

200, Perkin Elmer

Applications:



for determining very precisely the
concentration of a particular element in a
liquid sample. Can detect from 1 to 1000
µg/L.

For each element, the specific lamp is
required. Currently, Ca lamp is available.

The list of lamps/elements for analysis on
AAnalyst

200 can be found on
https://www.vwrcanlab.com/programs/equipment/
pdf/Model%20AAnalyst%20200%20Spectrometer
s.pdf



8

Biacore

is a methodology for measuring the
biomolecular

interactions in real time using
the phenomenon of
surface Plasmon
resonance (SPR).

Applications:
it allows to study:


Kinetics
of binding

• Solution affinity

• Steady state affinity

• Yes/No binding


Ligand

fishing

• Affinity ranking

• Protein concentration assays

• Protein percent activity assays

• Thermodynamics

Interactions between
nucleic
acids, lipids,
small
molecules, whole cells, and surfaces

can be studied.


BiAcoreX

SPR spectrometer

Specifications:


Two
Flow cell capability


Temperature regulation = 4
-
40
o
C


Flow rates
1
-
100μL/min
, steps of 1
μL


Required
sample volume: injection
volume
(min
-
5
μL

+
20
μl
)


Molecular weight detection: >180
Da


Refractive index range: 1.33
-

1.40



Sensitivity ≈ 20 RU, where
RU, resonance
units,
1RU
=
1
pg/mm
2

of sensor surface
.

9

Some applications are described in a paper by Y. Wei
and R. A. Latour,
Langmuir
2009,
25(10), 5637

5646

For more info:
http://web.bf.uni
-
lj.si/bi/sprcenter/BiacoreX.pdf


Materials Testing System (MTS)
Synergie

100

Testing:

Tensile
, Flex, Peel/Tear, Strain, and Compression test
methods.

1) Loadings available: 10 N (2
lbf
)

2) Speed
range: 0.001
-
1000 mm/min (0.00004
-
39 in/min)

3) Max
specimen thickness

for grips: 13
mm (0.5 in
)

4) 50 mm (2.0 inch) diameter lightweight compression
platens

Face
set, 25x25 mm rubber, for 100/2000 advantage
pneumatic and screw action
grips.


For more info:
http://www.mts.com/en/Bio/Materials/index.asp


10

HPLC/GPC system (Waters)

Applications:
high resolution

separations and purifications

Includes:

I.
Binary Gradient Pumping


System (1525)

I.
Autosampler

(2707)

II.
Photodiode Array Detector (2998)

III.
Refractive Index Detector (2414)

IV.
Column Heater

V.
Fraction Collector


Specifications:

I.
Binary
Gradient Pumping
System: Flow Rate
Range:
0
to 10
ml/min; max
operating
p
ressure
: 6000
psi.

II.
Autosampler
: Loop Volumes: 100 and 500 µl.

III.
Refractive Index Detector: Refractive index range: 1.00 to 1.75 RIU.

IV.
Column Heater: Temperature range: 20
o
C to 60
o
C; column capacity: up to
four 7.8 mm x 300 mm columns






For more info:
http://www.waters.com/waters/nav.htm?locale=en_US&cid=514256

11

Plasma cleaner/sterilizer, Harrick

For more info:
http://harrickplasma.com/applications_biomaterials.php


Applications:



Plasma activation of substrate surfaces to
render surface hydrophilic; promote attachment
and adhesion of functional biological species or
coatings to:

1)
Enhance cell adhesion, coverage, and
proliferation on tissue scaffolds.

2)
Promote adsorption of selective functional
biological species while resisting, adhesion
of bacteria and fouling microorganisms.



Removes organic contamination from surfaces.



Modifies or enhances the physical and chemical characteristics of surfaces: The
high energy plasma particles combine with the contaminant to form carbon dioxide
(CO
2
) or methane (CH
4
).

12