Icilin Behavior Protocol
mice and bring them to behavior room.
Weigh the mice and record their weights. To hel
identify the mice
later on, use a sharpie
and mark their tails. For example, mouse #2 has two marks on its tail.
Leave the mice in the behavior room and let them acclimate for 2 hours with white noise in the
mice are acclimating, suspend icilin in
1% Tween80 in PBS.
(Skip this step if you already have 1% Tween)
To make 1% Tween80
, make a
large volume in a 50mL conical
and vortex until Tween is completely dissolved
example, to make 40mL of the solution, add 39.6mL of PBS and 400
and vortex well
Based on the total weight
of the mice, determine how much icilin and 1% Tween80 you
The following formulas will make a
by 1.2 adjusts for
amount of icilin and 1% Tween
that you just calculated
into an eppendorf tube
and vortex. Icilin doe
need to be
wrap the tube.
mixture for 2 hours.
The sonicator is set only to 60 minutes
, so you
will need to start it again after an hour
. Before you
start the second hour of sonication
take out the icilin
mixture and vortex it lightly
After 2 hours of acclimating the mice, take the mice out of their cage
and acclimate them in the
for 20 minutes.
When ready to continue, inject the appropriate amount of icilin solution into each mouse
calculate how much icilin to use, follow this formula:
Each unit on the syringe is 10
L. For example, if you need to inject 90
L, you will need
When doing an intraperitoneal injection, make
sure the hole in the syringe is facing up. Go in at
about a 30
angle and aspirate (suck up) to make sure there are no colored fluids. If it is clean,
inject the icilin and place the mouse back in
the behavior box.
Make sure to put a piece of
n between the
boxes so the mice cannot see each other.
In between each experiment, clean up any mouse droppings
Stack two boxes on top of each other to prevent the mice from jumping out.
Observe the mice for 20 minutes
with sunglasses on.
the number of jumps and wet
shakes every 5 minutes.
After the experiments,
clean the boxes
thoroughly with the citrus spray and rinse them
off with the water spray. Clean the experiment surface in the same manner. Clean the desk with
At the end of every week, clean the boxes and lids with soap and water. Let them air dry
over the weekend.