Footprint-Free™ genetic engineering - Transposagen ...

familiarspottyΒιοτεχνολογία

10 Δεκ 2012 (πριν από 4 χρόνια και 6 μήνες)

187 εμφανίσεις

Better Models. Better Results.™
PiggyBac
PiggyBac
Transposon
Transposase
+
- PB Transposase + PB Transposase
“Cut”
GFP
GFP
Chromosomal DNA
“Paste”
at TTAA sites
Phase
Phase
PiggyBac
“Re-excise”
Transposase
+
Puromycin selection (10 μg/ml) 3 days
Footprint-free removal
“Re-excise”
Transposagen Biopharmaceuticals, Inc. | 145 Graham Avenue, A217 | Lexington, KY 40506 | TEL 859.428.8561 | transposagenbio.com