lab schedule - Ccsf

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1 Οκτ 2013 (πριν από 3 χρόνια και 8 μήνες)

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Day

Laboratory Exercise

(Instructions for students)

Links to required readings

In red: new or substantially revised

Teaching notes/To do suggestions for
instructors

in red: To Do for Crima before

WF add

1 day





Print before lab



Do "Before lab"
assignments in your
notebook or on BioPortal



Bring to class

Read/watch/do before coming to
class








15
-
Jan

16
-
Jan







You need to sign into your lab
section in S 315:



1/15: 2:10

with Prof. Pogge

1/16: 10:10
with Prof. Nixon

1/16: 12:10
with Prof.
Apollon





Take roll



Show appropriate notebook



Introduce website



Announce homework for next lab



Announce lab policies, absences

17
-
Jan

Introduction to the lab included
in print package 1, Scientific
method, Protocol
writing



Read: Hillis et al. Principles of

Life
,
concept 1.5 or Chapter 1 in Knisely



Read: Laboratory Safety



Read: Laboratory Notebook



Bring prepared notebook as
described in above file



Optional: Check out UC Berkeley's
site on limits of science



BioPortal quiz on Hillis reading

BioPortal quiz
-

i
ntroduction



Rewrite exercise: chapter 1 in
Knisely is really good. Include new
homework (see SLO activity) due
1/24



Review Introductory quiz questions



Check notebook at end



Grade open questions on BioPortal

22
-
Jan

Model cell
I included in print
package 1



See “Before coming to lab”



Check protocol (
-
2 if not)



Check question (from chemistry they
are used to copy “purpose”



Write homework model 1

24
-
Jan

Model cell II
-

included in print
package 1

Writing lab reports



See “Before coming to lab”



Write paragraph as outlined in Model
cell I (after lab)



Read: Laboratory reports

1 (includes
a rubric used by your instructor)



Check hypotheses



Go over homework findings
(abstracts). Discuss dependent,
independent, and control variables, as
well as positive and negative control (if
applicable). Refine research question.



Collect homework (4 points)



Assign protocol for investigation next
lab (or do yourself)

29
-
Jan

Data collection, organization, and
visualization
-

included in print
package 1




Write a brief introduction to our
investigation, use three properly
cited sources


paper copy due in
lab



Read: Plagiarism



Author guidelines for PLoS
submissions, includes how to
reference citations.



Read:
Rubric for lab report
1



Divide fi
le “lab reports in different
parts, assign Knisely, K. Writing in
Biology


free with textbook 1
-
Introduction and quotations), 2


Title
and results



Write rubrics for 1 and 2



Write intro assignment



Go over introduction, either peer
review or in the form of

ppt with bad
and good examples



Carry out experiment



Announce lab report 2 assignment, let
students know we will practice
graphing next lab



Revise lab report 2 write up, include
graphing,

31
-
Jan

Chymosin I
-





Read: (Micro)Pipettors (you need
this information for the quiz)



Do: Metric conversion tutorial



Review

Units and Measures
if
needed



Pre
-
lab pipet, metric conversion
and
graphing quiz

due on
BioPortal



Add graphing exercise to lab



Write graphing quiz for BioPortal



Do graphing exercise



go over pipetting



check “Test your understanding”

5
-
Feb

Chymosin II






Read: Laboratory reports

2 (
includes
a rubric used by your instructor)



Write: Lab report 2: Title and results


paper copy due in lab or submit
online according to your

instructor



New:
Student conferences lab report 2
or peer review or ppt with common
mistakes



Check for data table chymosin I

7
-
Feb

Introduction to microscopy

Introduction to chi square test of
independence



Read:
Microscopy background



Read: Chi square of independence



Pre
-
lab quiz on microscopes

and
chi square

due on BioPortal




Shorten microscope background



Write chi square of independence,
tailored to
investigation



Add chi square questions to quiz



Check chymosin II graphs (or do
during notebook eval)

12
-
Feb

Scientific literature I



Watch: Video of
experiment
(Science)



Optional: Article summarizing the
research report: Jailbreak rat
(Scientific American)



Submit revised result section,
include
chi square of independence
analysis



You might find it helpful to read p.
39
-
40 in
Writing in Biology



check article, paragraph numbers,
underlined words, definitions



Assign topics for bioethics

14
-
Feb

No lab



19
-
Feb

Bioethics




Optional: TED talk by Paul Root
Wolfe on an astonishing series of
recent bio
-
engineering experiments



Bioethics Presentation due



Bioethics talking points



Personal refl
ection



Bioethics quote



check quote (I do gallery walk) and
have students present on their topic


21
-
Feb

Mitosis



Watch: Mitosis animation



Read laboratory report 3
(discussion
and abstract)



Submit discussion/abstract for
investigation



Read:
Principles of Life pp. 128
-
132



BioPortal quiz on mitosis



New:
Peer review discussion and
abstract and/or instructor conferences
or ppt with good/bad examples

26
-
Feb

Meiosis, Chi square



Read:
Principles of Life pp. 134
-
141



Read chi square background



BioPortal quiz on meiosis


Rewrite qui square to distinguish from
independence

28
-
Feb

Amylase




Read: How City College and E. coli
got their amylase
-

by Dr. Sheri
Miraglia



Revise entire lab report incorporating
peer and instructor feedback submit
all drafts



Read: Centrifuges (you need thi
s
information for the quiz)



Pre
-
lab quiz on centrifuges due on
BioPortal



See “Before coming to class



check arguments for hypotheses



check test your understanding



check protocol

5
-
Mar

Genetics, Chi square goodness of
fit




Read: Chi square background



Read: Hilis, Principles of Life,
Appendix B



Pre
-
lab quiz on BioPortal



Introduce aseptic assignment



Could aseptic intro be take
-
home?



Write assignment part
for aseptic pre
-
lab

7
-
Mar

Lab midterm exam

Notebook due



Bring two abstract pertaining to
aseptic investigation that can serve
as background information




Lab exam:
justify your choice of
abstracts

12
-
Mar

Aseptic techniques




Discuss variables



Discuss data collection



Make sure enough data for statistical
analysis

14
-
Mar

Bioinformatics online lab, we will do
this in class together

Read up to "Questions to answer",
answer discussion questions in

your notebook.



See “Before coming to class”



After class:
Review: Bioinformatics
Power Point Presentation adapted
from Alexandra Schnoes



Write

review questions for
bioinformatics

19
-
Mar

PCR I






Watch: PCR animation (you need
this information for the quiz).



Pre
-
lab quiz on PCR due



21
-
Mar

PCR II




Watch: Electrophoresis animation



Read: Introduction to electrophoresis



Pre
-
lab quiz on electrophoresis
due



Full report on Aseptic lab is due,
including chi square of

independence, paper copy

2
-
Apr

Scientific
literature II



See “Before coming to class”



Article: Quantifying the Integration of
Quorum
-
Sensing Signals with
Single
-
Cell Resolution



Read:
Synopsis: Going With the
Glow: How Bacteria Integrate
Molecular Signals to Synchronize
Bioluminescence



TED talk by Bonnie Bassler (we will
watch this together in lab)



New: Instructor


student conferences
on lab report

4
-
Apr

GE I



See “Before coming to class”



Check table with reagents and
protocol



Rewrite
procedures

9
-
Apr

Autoinduction



Watch: Lux operon animation



If you loved Dr. Bassler's TED talk,
you might want to check out Dr.
Bassler's longer seminars
(Bioseminar, HHMI Holiday Lecture)



Revised lab report due



See “Before coming to class”



Check test your understanding



Pre
-
lab quiz autoinduction

11
-
Apr

Check
autoinduction results

GE II



Review: Introduction to
electrophoresis



See “Before coming to class”



Check protocol

16
-
Apr

Lifestyle analysis take home




Familiarize yourself

with this online
diet and exercise analysis tool



Nutritional research sites: e.g.,
American Society of Nutritional
Sciences and Harvard School of
Public Health



Discussion forum essay practice

Write discussion forum questions

18
-
Apr

GE II)

Scientific
literature 3, preparation



In a group of 3
-
4 students, choose
one of the articles below. Note that
often there are easier articles about
the articles available. Do a search!




Introduce oral presentation



Talk about group work



Review available articles

4/23

Check GE III results

Cardiovascular system lab

Sign up for presentation topic

BioPortal quiz on oral presentations



Lifestyle
quiz on BioPortal





Check protocol and material list



grade nutrition quiz



prepare sign up sheet for
presentations



write quiz on chaper 8 Knisely

425

No lab





4/30

Drugs and the brain



Pre
-
lab quiz on
BioPortal


(Re)write quiz on drug and the brain

5/2

ELISA



Pre lab quiz on oral presentation


5/7

Scientific Literature 3

-

Student
presentations I

Presentation rubric

(adjust to 32 points total)

+ 8 for participation in discussion
and assessments






Rewrite presentation rubric?



Rewrite instructions for sci lit III,
emphasize
reflection part

5/9

Student presentations II

Review for final






5/14

Lab final

Lab notebook due