Supplementary Data
Average Monosaccharide Composition
Calculations
•
Example data in this table originates from work done
for the following publication:
“Glycosylation of sputum
mucins
is altered in cystic
fibrosis patients”, 2007.
Glycobiology
, vol. 17 no. 7;
pp 698
-
712
•
The values are from a control sample.
•
The full composition of the patients glycosylation
profile is presented in the Table S1a, and an example
calculation for the
HexNAc
portion of this metric is
provided in Table S1b.
•
One composition is assigned to each mass, but there
may be more than one structure for a given
composition (e.g. 675.2).
•
The compositions are weighted using % abundance
(relative intensity).
Table 1a
Details of Control Patient Glycosylation profile as used to
calculate monosaccharide composition metric.
m/z
, mass to charge
ratio;
z
charge state of the ion;
HexNAc
,
N
-
acetylhexosamine
; Hex,
Hexose
;
Fuc
,
Fucose
;
NeuAc
,
N
-
acetylneuraminic
acid;
Sulf
,
sulfate
modification.
Table S1b
Sample computation of average monosaccharide
composition for one sample. (
HexNAc
residue). The intensities of
the structures are converted to percentages. Each instance of the
residue is multiplied by the % abundance, the results summed and
divided by 100.
Table S1c
Structures of the compositions found in example control
patient glycosylation profile (S1a &
b
). Structures were drawn
using
GlycoWorkBench
(
http://www.glycoworkbench.org/
) and use
the Consortium for Functional Glycomics (CFG) structure
nomenclature.
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