Chapter 9 – Biotechnology and gene manipulation

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1 Δεκ 2012 (πριν από 4 χρόνια και 8 μήνες)

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Chapter 9


Biotechnology and gene manipulation


Key Concepts




Tools and techniques

o

Gel electrophoresis

o

DNA profiling

o

DNA sequencing

o

DNA recombination

o

DNA amplification

o

Gene cloning

o

Gene transformation

o

Gene delivery systems



Task

Co
mpleted


Qns: 1,2,3,4,
5,6,9,10,15,16,17,19,21,27,29,34,35,36,37,38,43


Apply Understanding Qns: 1,2,3,4,7



Look at the ethical issues surrounding the use of gene cloning, gene
transformation or gene delivery. Write a half page summary




Make an annotated diagram, distinguis
hing between sticky and blunt
ends.




Construct a flow diagram summarising the process of PCR




Draw an annotated diagram showing the process of gel electrophoresis
and the results. Include the equipment you need, and how the process
works.




Create
a VENN diagram looking at transferring genes using vectors
(Viral vectors, Liposome vectors, Plasmid vectors)




Create a concept map showing the different applications of biotechnology

(Eg Gene Technology and Cancer, Malnutrition, Biotechnology &
Medicin
e, Genetic testing, Production of pharmaceuticals, gene therapy,
Agricultural and environmental applications of biotechnology, Cloning,
Stem cell technology





Create study cards on the following topics:




Biotechnology



Genetic engineering / gene technolog
y



Restriction Enzymes (also known as Restriction endonucleases)



Restriction fragments



Recognition sites



DNA ligase



Recombinant DNA technology



Blunt Ends, Sticky Ends



PCR



Gel electrophoresis



Gene probes



DNA sequencing



Gene cloning


using plasmids



Plasmids



Transferring genes (Viral Vectors, Liposome vectors, Plasmid
vectors



DNA profiling (STRs) (VNTRs)



Applications of Biotechnology



Cloning



Stem cell technology



Implications and issues of gene technology (Ethical, Medical)