Evaluation of a new method for antifungal susceptibility testing for ...

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Evaluation of a new method for antifungal susceptibility testing for
azoles







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Author(s):

Sabra W

(Sabra, W.)
1
,
2
,

Tawfik AF

(Tawfik, A. F.)
1
,

Shibl AM

(Shibl, A. M.)
1

Source:

WORLD JOURNAL OF MICROBIOLOGY &
BIOTECHNOLOGY

Volume:

26

Issue:

3

Pa
ges:

451
-
457

Published:

MAR 2010


Times Cited:

0

References:

23

Citation Map


Abstract:

The interpretation of the end points in azole antifungal drug suscep
tibility testing is
challenging, in part due to incomplete growth inhibition of Candida species. Since the reference Clinical
and Laboratory Standards Institute (CLSI) broth microdilution method have limitation with azoles, a new
modification of the CLSI m
icrodilution protocol was evaluated. We measure the decrease in growth rate
(mu) of exponentially growing cultures in accordance to different azole concentrations at time intervals
up to 10 h. Using 15 different Candida strains, an overall agreement within

+/
-

A 2 dilutions by the CLSI
method at 24 h in RPMI and the mu
-
dependent method for three antifungal agents (fluconazole
-

itraconazole and voriconazole) was achieved. MIC measurement by the new method was less sensitive
to the medium used or the inoculum

size applied. The presented data suggested that, measuring the in
vitro inhibition kinetics at the logarithmic phase could have advantages for addressing susceptibility
testing toward azoles.

Document Type:

Article

Language:

English

Author Keywords:

Tr
ailing; CLSI broth microdilution method; Azoles; Growth rates; MIC; Candida

KeyWords Plus:

CANDIDA
-
ALBICANS BIOFILMS; CRYPTOCOCCUS
-
NEOFORMANS; BROTH
MICRODILUTION; DRUG
-
RESISTANCE; IN
-
VITRO; GROWTH; FLUCONAZOLE; ASPERGILLUS;
MICAFUNGIN; RESPONSES

Reprint

Address:

Sabra, W (reprint author), King Saud Univ, Coll Pharm, Dept Pharmaceut, Microbiol
Unit, POB 2457, Riyadh 11451, Saudi Arabia

Addresses:


1. King Saud Univ, Coll Pharm, Dept Pharmaceut, Microbiol Unit, Riyadh 11451, Saudi Arabia


2. Univ Alexandr
ia, Fac Sci, Dept Microbiol, Alexandria, Egypt

E
-
mail Addresses:

waelsabra@web.de

Publisher:

SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA

Subject Category:

Biotechnology & Applied Microbiology

IDS Number:

556Z
F

ISSN:

0959
-
3993

DOI:

10.1007/s11274
-
009
-
0188
-
y