JS 196e: Scientific Basis of DNA Typing continued and Methods ...

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14 Δεκ 2013 (πριν από 3 χρόνια και 6 μήνες)

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JS 196e: Scientific Basis of DNA Typing continued
and Methods used to Study DNA

I.
Pre class activities

a.
Quiz

b.
Assignment and announcements


II. Learning Objectives


Definition and Inheritance review

Introduction to DNA
-

continued

a.
DNA structure

b.
Be able to draw DNA structure


Base Pairing AT, GC

c.
Understand DNA replication

Summary of Introduction to DNA


Overview of Methods used in Forensic DNA typing


Screening


Extraction


Quantification


Assignments and
Announcements


Friday 9 Feb: Guest lecture by Dr. Chris Chen of CCNY on
nanotechnology
-

1 point extra credit for attending and
writing a 500 word summary


Required assignments for 12 Feb


Work on Laboratory Notebooks


Study for a quiz


Read Butler C3/InmanC2


Review : Special collection guidelines for Biological Evidence
-

http://www.cacnews.org/wordfiles/DNA%20SampleHandling.doc


Where’s Daddy?

Father

Child #1

Child #2

Child #3

Mother

PCR product size (bp)

11

14

11

12

14

8

14

12

12

8

Inheritance Review


Dad is homozygous (A,
A) and mom is
heterozygous (A, a).


In your teams, draw the
Punnett Sqaure that
demonstrates the
inheritance of these
alleles.


What percentage of their
children will be
heterozygous?

DNA

Structure

What is it?

Bases (AGCT) form the stairs of the ladder, are
faithfully paired and exhibit
differences.

P


S
-
A : T
-
S

P P

S
-
G : C
-
S

P P

S
-
A : T
-
S

P P

S
-
G : C
-
S


P


Sugars (S) and phosphates (P) form the sides of the ladder (
identical for all
DNA
).

Bases (AGCT) form the stairs of the ladder, are faithfully paired by hydrogen
bonds and exhibit differences.
A : T and G : C



A = T

G


C

T = A

A = T

C


G

T

C

C

A

G

G

T

A

G

C

T = A

T = A

C


G

A = T

A = T

G


C

5’

3’

3’

5’

3’

3’

5’

denatured

strands

hybridized

strands

Hydrogen bonds

C


G

C


G

G


C

Phosphate
-
sugar backbone