Course Number: - Montgomery College

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Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring200
4©2004


BSMaT

-

page
1

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Course Number:

BT 200

Course Name:


Protein Biotechnology

Semester:


Spring 2004


I. Instructor Information

Professor Name:



Dr. Collins Jones

College Phone Number:


301
-
353
-
1910

Division Office Phone Number:

301
-
353
-
7722

College email address:


collins.jones@montgomerycollege.edu







Office Location:



Room 421 HT Building Germantown Campus






20200 Observation Drive






Germantown MD


Professor Name:



Dr. Lori Kelman

College Phone Number:


301
-
601
-
6929

Division Office Phone Nu
mber:

301
-
353
-
7722

College email address:


lori.kelman@montgomerycollege.edu







Office Location:



Room 418 HT Building Germantown Campus






20200 Observation Drive






Germantown MD







Office Hours:




TBA


II. General Cours
e Information


Course Title:


Protein Biotechnology

Credit Hours:


4


Course Number, Meeting Times and Location:


Day (Kelman)



CRN

35743


Lecture:

Monday

9:00 AM
-

11:40AM

Room HT 405

CRN

35744


Laboratory:

Wednesday

9:00 AM
-

11:40 AM

Room HT 405



Evening (Jones or TBA)


CRN

33973


Lecture:

Monday

6:30
-

9:10 PM

Room HT 406

CRN

33974


Laboratory:

Wednesday

6:30
-

9:10 PM

Room HT 405

Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring200
4©2004


BSMaT

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page
2

of 20
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Course Format:

Lecture and Laboratory Exercises


Prerequisites:


CH 101, BI 107 (prefer BT 115, BT 117 and Ch
em 120 or Chem 203)


Co
-
requisites:


none


Tentative Summary of Topics to be Covered:

Please refer to the course syllabus and








college catalog description


III. Specific Outcomes

On completion of Protein Biotechnology students should have a basic
understanding of the
structure and function of proteins. For example the student should be able to define primary,
secondary and tertiary protein structures and how to determine each. The student should be able
to successfully perform protein quantitation

assays, enzyme assays, PAGE gel electrophoresis,
basic protein purification steps using chromatographic techniques. The student should be able to
describe and carry out functional assays to measure the biological activity of proteins. The
student should

understand and apply basic principles related to protein purification and
manipulation. They should understand principles of protein engineering, expression and peptide
synthesis and be aware of industrial considerations related to proteins production fo
r diagnostic
or therapeutic use.


IV. Text and Supplies


Textbook:

Handouts will be supplied as needed. There is no suitable text for this




course at this level. The student is encouraged to use basic




biotechnology, biochemistry and molecular biol
ogy texts as




supplementary references.



Lab Manual :

Short Protocols in Molecular Biology (4th or 5
th

edit.)

(1999
-

2003).




Ausubel, et al. John Wiley and Sons, New York, N.Y.



Additional Required Materials :

*Safety
Goggles

(must be department
approved)







*Lab Coat







†Scientific Calculator







*Lab Notebook (department approved)







*‡Pen (Black ink only)





*


Items to be obtained before second laboratory







Each lab bench is equipped
with two scientific calculators for use in

the laboratory
-

please do not remove them







Each student will receive a black ink pen on the first day of
Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



P
rotein Biotechnology Spring2004©2004


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laboratory.

V. Grading


A. Requirements


The following requirements must be met in order for a student to pass this course regardless of
th
e student’s grade average:




Attend all laboratories and complete the laboratory exercises ( maximum
of three excused absences allowed)



Complete and turn in assignments



Complete and turn in a laboratory notebook according to specifications for
keeping a no
tebook in the Biotechnology Program



Take and pass all exams



Complete a research paper as assigned in selected Biotechnology classes

B. Course Grade


The final grade for this course will be determined based on the following evaluation criteria :



1. Quiz
zes and Exams


2. Assignments


3. Laboratory Notebook


4. Laboratory Evaluation


5. Research Paper in select Biotechnology classes


6. Final Exam


1. Quizzes and Exams.

1a. Quizzes :

A quiz may be given at any time
-

announced or
unannounced

-

in l
ecture
and / or lab. Quizzes will be considered a means of evaluating your
understanding of the subject material or to ensure you have memorized required
material. Five to eight quizzes, each worth 20
+
10 points will be given in lecture
and lab during the

semester.


1b. Exams : Two exams, each worth approximately 150
-

180 points, will be given
during the semester. Exam questions will be derived exclusively from material
presented in lecture and lab. Emphasis will be placed on analytical thinking /
prob
lem solving skills, but some definitions, multiple choice or short answer /
essay may also be included.
All exams will include at least one page of
laboratory calculations.



Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomeryc
ollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

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4

of 20
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For any quiz or exam all work must be shown, especially when solving problems. A
nswers
only will receive one point.


1c. NO MAKE
-
UP Quizzes or Exams will be given unless appropriate documentation is
presented. If a make
-
up exam must be administered
the additional time the
student has to prepare will be taken into account in selecti
ng appropriate
questions for the make
-
up exam.


NOTE 1:

All material for quizzes and exams will be derived only from content presented in
the lecture and laboratory. You are responsible for this material present or not




-

some material presented is not
covered in the textbook.


NOTE 2:


The use of personal electronic devices other than a calculator will
not

be
permitted during quizzes or exams. For example you may
not

use a cell phone or
PDA during exams even if you say it is for the calculator functio
n. Only the use
of a dedicated calculator is permitted during exams or quizzes. No exceptions.


2. Laboratory Notebook.

A laboratory notebook must maintained by each student when enrolled in a laboratory course in
the Biotechnology Program. The noteboo
k, format and hints on how to record information in the
book will be discussed during the first class. The notebook may checked at any time for
completeness so be sure to bring it to each class beginning with the second laboratory. Grading
will be based
on adherence to format, clarity and legibility, completeness, accuracy, timeliness of
entries, ... All entries must be immediate and permanent.


3. Laboratory Evaluation


Each student taking a Biotechnology Laboratory will be evaluated by his or her in
structor during
the course of the semester. The specifics of the evaluation will be kept confidential. Evaluation
criteria will include:





Time management :

Starts lab on time?







Completes lab on time?







Organizes/ plans work time during the l
ab?




Attentiveness:


Listens to pre
-
lab and in
-
class directions or does he







repeatedly ask questions on items already covered ?

Pays attention to protocols and carries out directions







properly
-

able to follow written and oral directions?







Asks for clarification when confused (a good







thing!)?







To class on time and focused on classroom activity?

Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

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Preparation:


Does the student come to class prepared
-

lab







notebook, lab coat, safety goggles, black ink pen,

Knowledge of pro
tocol (e.g. what is the title, what
reagents and equipment will be needed?)




Behavior / Attitude:

Participates /Enthusiastic
-

does fair share of work?

Treats instructor, other students and lab
environment(equipment, reagents,...) with courtesy

and resp
ect?







Able to work as a team member
-

collectively and







individually ? Asks for and gives assistance as

needed?







Obeys rules of laboratory
-

safety, reagent handling,







...?




“Laboratory Hands”:

Demonstrates proper dexterity, eye
-
hand








coordination to successfully perform basic







laboratory tasks?







Able to competently use and understand basic





Laboratory equipment such as BSC, pipetteman,





pipette aid, balance, electrophoresis apparatus,





spectrophotometer,.
. ?





laboratory evaluation
-

a self evaluation and an evaluation by your lab




partner will be completed at the conclusion of each lab


4. Research Paper


In some biotechnology classes a short paper (5
-
10 pages) will be required. The papers must be
written using a word processor
-

hand
-
written papers will
not

be accepted. The topic, source
material and format of the paper will be discussed in the respective courses.


5. Final Exam



The final exam will be
cumulative

for the entire semester and wil
l include both lecture and lab.
Every student

must take the final exam.

The final will be worth about 275
+

75 points. The final
exam is an extended, comprehensive version of the “hourly” exams. The questions will be
original (i.e.
not

derived from pre
vious exams). Emphasis will be on integrating various topics
covered in lecture and lab over the entire semester.





Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


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C. Standards.

The criteria for assignment of

a letter grade is as follows:




Exam Points







300
-

360



Assignments







50
-

150



Quiz Points







50
-
150



Lab Points (includes evaluation and notebook)


200
-
300



Paper (when assigned)





75



Final Exam



250




Total possible points for semester




925
-
1285


If you wish to es
timate your letter grade during the semester simply add up all of the points from
lab reports and exams returned thus far, divide by the total number of points possible and
multiply by 100 to obtain a percentage (%).



% =



your total points


x 100




total possible points



%






%

90
-
100

= A



70
-

79

= C

80
-

89

= B



60
-

69

= D


At the end of the semester I will draw up a curve based on the TOTAL number of points for each
student. Any student with 90% or greater will rece
ive an "A", 80%
-
89.99% a "B" and so an.

Quizzes and Exams will
not

be curved individually. “Extra credit” assignments to raise a grade
will
not

be offered or accepted.


D. Make
-
up Policy


1. Lecture and Laboratory Information

The student is responsib
le for obtaining lecture notes, handouts and other pertinent information
for any lecture or laboratory material missed. If you know you are going to be absent call a
classmate and ask that individual to obtain extra handouts. The instructor will not reta
in extra
copies of handouts from week to week. Similarly lecture and/or lab notes must be obtained from
a fellow student. The instructor will be happy to answer questions about the material during
regularly scheduled office hours.





2. Quizzes and Exa
ms.

Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

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NO MAKE
-
UP Quizzes or Exams (including the final exam) will be given unless appropriate
documentation is presented. If a make
-
up exam or quiz must be administered
the additional time
the student has to prepare will be taken into account in selecting a
ppropriate questions for the
make
-
up exam.


3. Laboratories.

There are
no make
-
up labs

for Biotechnology courses. Time and budget do not permit make
-
up
labs. The laboratory materials are expensive and often have a short time during which they are
usable

-

usually one or more of the substances used in any given laboratory exercise will no
longer be viable after 24 hours. If more than one section of a Biotechnology Laboratory is
offered the student must request permission from both instructors to make up
the lab.
Permission is at the discretion of the instructors

and is also dependent on space and reagent
availability. The make up lab must correspond to the lab scheduled for that week.


4. Incompletes.

Only under the most extenuating of circumstances wi
ll an incomplete (grade of “I”) be issued.
Proper documentation must be presented and the grade must be completed within one semester
of the incomplete issuance. If the incomplete is not completed within one semester the grade is
automatically converted
to “F” (college policy).


E. Late Policy

Points will be deducted for any work handed in late (e.g. take home exams, research paper). 5%
of the total possible points for that assignment will be deducted from the score for each day the
work is late, includ
ing weekends and school holidays. Work that is more than 7 days late will
not be accepted. Work due at the end of the semester must be handed in by 5:00 PM the last day
of final exams
-

work handed in after that will be not be accepted or graded.


F. Au
dit Policy


Students who audit Biotechnology lecture classes are not required to take quizzes or exams but
are welcome to do so. Students who audit the laboratory section must accept the same
obligations and responsibilities as students who take the class

for credit
-

they are expected to
participate in all aspects of the laboratory experience such as keep a notebook, be prepared, take
quizzes, demonstrate regular attendance, work as part of the team ...






VI. Classroom Policies


Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery College

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


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A. Attendance and
Withdrawal from Class

1.

Lecture



Strongly encouraged. Material will be covered that is
not

presented in the text or on handouts.
You are responsible

for all material covered in lecture
-

present or not
, including any handouts
or homework problems. In
structors do
not

keep extra material

for the following week. If you
are absent or late without an excuse and miss a quiz or exam you will receive a “zero” for that
test (also see below on practicums / internships). Be aware that changes in exam dates and

other
scheduling adjustments may be announced during lecture
-

it is
your

responsibility to be aware
of any changes. Multiple / consecutive absences are noted. A student who is repeatedly absent
without a valid excuse from lecture will receive the lowe
st possible priority with respect to
assistance and office hours. All quizzes will be given and collected within the

first twenty
minutes of class
-

if you are absent or late you will miss the quiz and will receive a zero for that
quiz. (Note : If you com
e late you must still complete the quiz in the first twenty minutes.)


2.

Lab


ATTENDANCE IS ABSOLUTELY REQUIRED !!





There are be no make
-
up labs for Biotechnology courses. The laboratory



materials are expensive and often have a short time during whic
h they are usable
-



usually one or more of the substances used in any given laboratory exercise will



no longer be viable after 24 hours. Budget and time constraints do not allow for



the purchase of additional material.




Each absence will be counted

as a zero;
3 unexcused labs

during the
course of the semester will result in a
failing grade

for the lab

and by default, in
compliance with departmental policy, a failing grade for the
entire course
. This
policy will be strictly adhered to.




Latecomers

or unprepared

students will be sent home and considered
absent. The unprepared student is a danger to all who are working in the lab. See
prelab requirements.



3. For Students Enrolled in the Biotechnology (BT) Program


A. Attendance

All students enr
olled in BT courses will be asked to sign an attendance sheet for each class
(lecture and lab). An important part of the Biotech program provides the option for students to
spend a minimum of 160 hours at each of the Practicums at a specific off campus jo
b site. While
at the Practicum each of you will represent the entire Biotechnology program at Montgomery
College
-

if you fail to live up to your obligations you will not only fail the Practicum but you
will also compromise chances for future students to

participate at that particular job site. One of
the most important criteria in obtaining departmental permission and support to participate in the
Biotechnology Practicums will be your attendance record in BT classes. Therefore, in order to
Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

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9

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-




have documen
tation of your attendance (and thus a prediction of your practicum attendance)
attendance will be recorded for each class by placing a "sign in" sheet on the front desk at the
start of each class. Students with a poor attendance record (absent more than
2 times) or
students who are consistently late will not be recommended for participation in the Practicums
by the BT coordinator (i.e. not have department support in locating a work site
-

the student will
be on his or her own).
Please note
: students sl
eeping in class will be considered absent
-

if you
are that tired go home. You are welcome to come back and take our classes when you are
awake. Attendance (or lack of) in lecture will
not

influence your lecture grade
-

only the level of
assistance you re
ceive in locating a Practicum site. Attendance for laboratories is mandatory
(see above). Also students with
repeated

absences will receive the lowest priority with respect to
office hours.


B. Academic Honesty

Students in the Biotechnology Program will

be held to the highest standards of academic
integrity. Human lives can depend on your honesty and your commitment to record and report
your own data and answers truthfully. All students enrolled in the Biotechnology program are
expected to do their own

work on homework assignments, quizzes, exams, papers and laboratory
notebooks. Cheating in any form (copying from exams, falsification or copying of data,
plagiarism, purchased reports, ...) is not acceptable. It is critical that our assessment of your
knowledge level and laboratory ability is accurate so that a correct placement can be made. Any
student who obtains a higher grade through undetected cheating will not only cheat themselves
and potentially be placed at a position above their ability (likel
y resulting in being fired from the
job) but may also place lives in jeopardy.
Therefore cheating, plagiarism or any other form of
academic dishonesty will not be tolerated. Any student demonstrating such lack of academic
integrity will receive a failing

grade (“F”) for that course and be permanently withdrawn from
the Biotechnology program by the program coordinator.




C. Classroom Conduct


All students are expected to abide by the Student Code of Conduct as set forth in the student
handbook. This inc
ludes, but is not limited to, academic honesty and appropriate behavior in and
out of the classroom while on college premises. Students who are disruptive to classroom
activities or who fail to display proper conduct towards other students or faculty will

be required
to leave the classroom. Such violations will result in a referral to the Dean of Students. That
student will not be allowed to return to class until he or she can behave in accordance with the
Student Code of Conduct. In addition such behav
ior will be reported to the program coordinator
as unprofessional behavior and will adversely affect the student’s chances for placement at
internships / employment opportunities.
Please turn off all beepers, cell phones, etc before
entering the classroom
. 10 points will be deducted from your final point total for the semester
Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


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-




the first time your cell phone or beeper is heard during class time (lecture or lab), 20 points the
second, 40 points the third and so on.

You may set your beeper or cell phone to a

non
-
audible
signal (e.g. vibrate) and
respond to emergency calls outside of the classroom
.

























D. Support Services


The Germantown Campus offers numerous support services to assist your educational progress.
These include :


1. Scie
nce Learning Center (301
-
353
-
1937)


The Science Learning Center (SLC) is located in Room 202 of the Science (SAS) building and is
administered by Dr. Megan Birney. Dr. Birney is knowledgeable in Biotechnology and can offer
assistance when I am not availab
le.



2. Math Learning Center (301
-
353
-
1947)


The Math Learning Center is located in Room 229 of the HT building.

Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


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3. Writing Center (301
-
353
-
1930)


The Writing Learning Center is located in Room 107 of the Humanities building.


4. Counseling Center (3
01
-
353
-
7770 / 7734)


The Counseling Center is located in Room 175 of the Science (SAS) building.



E. Cancellation of Classes


Classes will be canceled in accordance with college policy. If in doubt check the coordinators
voice mail or the BSMaT office.













VII. Additional Comments


A. Scheduling of Lecture and Laboratory Times


The focus of the Biotechnology program is to train students to become successful laboratory
workers and to prepare students for future academic success. In order to pro
vide students with
the maximum learning experience times designated for lecture and laboratory may be
interchanged and/or mixed. For example it may be necessary to inoculate a culture, pre
-
swell a
column or pour a gel before using these items in the lab.

In such situations we may meet in the
laboratory during a scheduled lecture time to initiate the experiment and then continue with
lecture. All meetings will occur only during the designated lecture and laboratory times as listed
in the schedule of class
es.

Announcements regarding scheduling (changes) will be made during lecture and lab, usually one
week in advance. In general the experiments are designed to be completed in the time allotted.
However unprepared students, students who do not follow dir
ections or make a mistake may run
Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


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over the allotted time. Students will only be allowed to work a maximum of
45 min past

the
scheduled conclusion of any laboratory.


B. Homework.


Homework may be assigned from both the text and supplementary handout prob
lems when

available. Homework will
NOT

be collected or graded
-

it is strictly for your practice. As time
permits answer keys will be provided for supplementary handout problems. For best results do
the homework as it is assigned. Homework assignment
s will
not

be answered during lecture or
lab time so

please be sure to ask for help
with any problems

after

any class or during office
hours.















C. Office Hours


If you have a question or need some clarification please do not hesitate to ask.


Ask as soon as you have difficulty
-
don't wait until the last minute !!

The instructor will be happy to answer any questions or go over homework problems
immediately
following

any laboratory (within reason) or during scheduled office hours
-

see
posting
at HT room 418 or 421 or by appointment as other duties permit.


If you plan to come to office hours please call so that the instructor can be sure to be in the office
when you arrive. When you call please be sure to leave the following information :





Your name




Your phone number (speak slowly and
repeat

it)




Times you may be reached


One hint to make efficient use of time is to come with specific questions already prepared. Some
Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


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-




students have a written list. Mark those sections in your notes or o
n your homework where you
had difficulty. A little time organization will go a long way!


We will also respond to e
-
mail :

collins.jones@montogmerycollege.edu







Lori.kelman@montgomerycollege.edu



















VIII. Course Sch
edule.


Please Note:

The course syllabus is a

tentative schedule

of lecture topics and exam dates. The
exact schedule is subject to change. One major factor affecting the pace at which
the syllabus is covered is the student. Topics are arranged sequenti
ally and thus it
is often necessary to be sure that one concept is covered and understood before
continuing to the next topic. Please keep your in class questions focused and brief.
I will be happy to answer
specialized, tangential or interest

questions
a
fter

class
or during my office hours !


A. Tentative Lecture Syllabus


Week of


Lecture Topic


January 26


Introduction / Course Policies





Overview of Protein Biotechnology
-

Proteomics




Properties of Amino acids


Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

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of 20
-




February 2


Properties of Amino Ac
ids




Primary Structure of Proteins
-

Peptide bond



Peptide synthesis / combinatorial peptide chemistry




Secondary Structure of Proteins






February 9


Secondary, Tertiary and Quaternary Structure of Proteins


February 16


Methods for the Determinati
on of Protein Structure




Protein Bioinformatics


February 23


Protein Synthesis / Post
-
Translational Modifications


March 1


Exam I


March 8


Protein Synthesis
-

Expression Systems and Scale
-
up

March 15


Protein Function
-

Enzymes and Receptors


March 22
-
26


Spring Break No Classes!




Biotech Trade Show at Montgomery College Germantown


March 29


Protein Function
-

Regulation





A. Tentative Lecture Syllabus


Week of


Lecture Topic

April 5



Protein Purification


April 12


Protein Purification


Apri
l 19


Exam II


April 26


Protein Sequencing, Bioinformatics and Structural Determinations




May 3



Industrial Protein Production
-

Scale
-
up Operations / cGMP and
Production Considerations



Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

-

page
15

of 20
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May 10
-
16


Final exams
-

Final Exam is Cumulative for the entire

semester
lecture and lab
-

every student must take the final exam

____________________________________________________________________________


Important Semester Dates for Spring 2004:


January 26


Classes Begin


February 1


Last day to drop date and get

a refund


February 15


Last day to drop without a grade being recorded or change audit /




credit


March 19
-
27


Spring Break College Closed
-

No classes, No faculty on Campus!!


March



Biotech Trade Show Association on Germantown Campus


April 1



La
st day to file for Spring Semester 2004 graduation


April 19


Last day to drop classes with a "W"


May 10
-
16


Final Exams



June 1



First Day of Summer Session I.


____________________________________________________________________________


B. Tentati
ve Laboratory Schedule


Week of


Laboratory Topic






January 26


Introduction to Biotechnology Laboratory




Safety, Equipment, Volumetric Measurements




Solution Calculations


February 2


Properties of Amino Acids




Preparation of Buffers


February
9


Tissue / Cell Lysis for electrophoresis




Determination of Serum Protein Concentration
-






Coomassie Blue Assay





BCA assay

Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

-

page
16

of 20
-







Practice Page Gel Loading






February 16


Determination of Protein Concentration in cell lysate




Protein Assay Cal
culations and aliquoting




PAGE run of Serum Sample
-

load optimization


February 23


SDS
-
PAGE of Lysate




Run 1
st

dimensional gel (IEF) of lysate




Methods of Protein Concentration :

Dialysis









TCA precipitation




Preparation of Homogenization

buffer for functional protein recovery


March 1


Run 2
nd

dimensional gel (SDS
-
PAGE) of lysate




Homogenization of tissue / cell samples for functional analysis




Catch
-
up / Summary

March 8


Enzyme Assays
-


Alkaline Phosphatase
-

optimization of assay
c
onditions







Acid Phosphatase
-

fixed point assay


March 15


Enzyme assasys
-

Alkaline Phosphatase properties
-
high throughput screen
for inhibitors




Analysis of Data





B. Tentative Laboratory Schedule


Week of


Laboratory Topic



March 22
-
26


Spring Break
-

No Classes!


March 29


Tissue Homogenate:

Protein Determination




Alkaline Phosphatase Assay of Tissue




Protein Purification Demo with Pharmacia program




Begin hydration of Sephadex


April 5



De
-
fine, Pack and run Sephadex column




Ch
eck fractions for Alkaline phosphatase activity




Run PAGE of homogenate at various loading amounts




Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

-

page
17

of 20
-




April 12


Batch Ion Exchange of Pooled Sephadex fractions




Catch up


April 19


Begin analysis of Ion Exchange fractions


April 26


Complete analysis
of fractions




Begin Peptide Mapping of Protein Sample


May 3




Compete Peptide Mapping Tricine
-
PAGE




Lab clean up and review.





Lab Notebooks are due on Wednesday May 5
-

Late Notebooks will not
be graded
-

no exceptions


May 10
-
16


FINAL EXAMS
-

N
O LABORATORY

____________________________________________________________________________


Please note:

The lecture and laboratory schedules are tentative and
will depend on the progress of the class are subject to
modification at the discretion of the i
nstructor.









Paper


A 10
-
15 page paper with references will be due on or before December 6, 2001. The
paper must be word processed using 12
-
point font on 81/2 x 11 paper. References, tables, charts,
diagrams do not count as part of the 10
-
15 pages
. For references you may use text books,
magazine articles or (preferably) scientific review articles. The references should be listed in
scientific format,
not

as in an English paper (no "ibid").


The reference should be listed in the paper by author o
r editor separated from the
publication year by a comma each time it is used. At the conclusion of the paper the complete
reference is given in the reference section and references are listed in alphabetical order using the
first authors name.

Your paper

must have at least five (5) references.


Sample reference format "Tn5, like Tn10 is thought to transpose by a conservative cut and paste
mechanism (Berg, et al., 1989)."

Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

-

page
18

of 20
-





complete reference listing for reference section :

Berg, D.E. (1989). Transpoon Tn
5. In "Mobile DNA" (D.E. Berg and M.M. Howe, eds.),


pp. 185
-
210. American Society for Microbiology, Washington, D.C.


Important sections to be included in your paper and labeled as such :


Title Page with your name

Background and introduction



overview
of the discussion
-

what points will you cover / make



why is this topic important



what does this reader need to know to understand the rest of your presentation

Overview of methodology




non
-
detailed description of methods / technology



what steps a
re involved what must be done at each step correct order of steps



what problems may be encountered how to deal with problem

Ethical issues



what is the impact of this method on individuals and society, should this



technology be used, what are the prop
er and improper uses of this technology,



should it be regulated or controlled (limited access) how can this be done

Summary

-



what important points and issues have you made that you want the reader to


“Walk away with” ? What did the reader learn ?

what are the future directions ?

References





Sample Topics for Paper


Role of proteins in health and disease




Alzheimer disease




Cystic Fibrosis




Sickle cell anemia




Phenylketonuria




Cholesterol / atherosclerosis / lipoproteins




receptor /

ion channel defects




drugs for the inhibition or stimulation of proteins





cholesterol regulation





anti
-
psychotics





Alcoholism




enzyme assays for disease diagnostics




enzyme replacement therapy

Dr. Collins Jones









Biotechnology Program

Room 421 HTSC









Montgomery Col
lege

301
-
353
-
1910




Course Syllabus BT
-
200



Germantown, MD

collins.jones@montgomerycollege.edu



Protein Biotechnology Spring2004©2004


BSMaT

-

page
19

of 20
-







prions / Mad Cow Disease




inborn metabolic

errors


Protein Microarrays


Industrial use or production of proteins




biotechnology research




food industry




enzymes for the manufacture of pharmaceuticals or speciality chemicals




protein engineering
-

design, purification or structure solutions



Environmental applications of enzymes




biosensors




bioremediation


You may choose other topics providing they are approved by the instructor.


You may not prepare a paper on anything related to the immune system. The paper must focus
on proteins (pl
ease limit any discussion of gene sequences or expressions
-

only as they relate
to protein function as you be asked to prepare a paper for BT 213).


Important Assignment Due Dates :



April 5




Topic Selection Due


April 19


Outline or Abstract Due


May

3




Paper Due