Biotech
Methods
BITC
1402
Dr. Larry Loomis
-
Price
Spring 2008
Who am I?
Dr. Larry
Labman
Biotech is a lab science
What is Biotechnology?
What is Biotechnology?
Oldest forms of
biotechnology
techniques for making
yogurt and cheese
(with bacteria); bread,
beer and wine
(yeast).
What is Biotechnology?
The use of living
organisms or parts
or organisms to
make a useful
product or as part
of an industrial or
technical process
What is Biotechnology?
•
Newer products of the
biotech industry are
found in many fields,
including the “5 F’s”:
•
Food
•
Fiber
•
Fuel
•
Filtration
•
Pharmaceuticals
We Use Instruments
-
Small
•
Hot plate/stirrers
•
Pipettors
•
pH meters
•
Microscopes
•
Minifuges
•
Balances
And
Large (next semester)
•
Spectrophotometers
•
Electrophoresis rigs
•
HPLC’s
•
Mass Spec’s
•
DNA sequencers
•
nmr
, X
-
ray etc.
TOP PAPERS PUBLISHED
OF ALL TIME
1.
O.H. Lowry et al.
"Protein measurement with the Folin phenol reagent, "J
Biol Chem,
193:265
-
75, 1951.
293,328
2.
U.K. Laemmli
"Cleavage of structural proteins during assembly of head of bacteriophage T
4
,"
Nature,
227:680
-
5, 1970.
192,022
3.
M.M. Bradford
"Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein
-
dye binding,"
Anal Biochem,
72:248
-
54, 1976.
120,179
4.
F. Sanger et al.
"DMA sequencing with chain
-
terminating inhibitors,"
Proc Natl Acad Sci,
74:5463
-
7, 1977.
63,909
5.
P.Chomczyski,N
.Sacchi
"Single
-
step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction,"
Anal
Biochem,
162:156
-
9, 1987.
55,987
6.
H. Towbin etal.
"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some
applications,"
Proc Natl Acad Sci,
76:4350
-
4, 1979.
48,671
7.
J. Folchetal.
"A simple method for the isolation and purification of total lipides from animal tissue, "J
Biol Chem,
226:497
-
509, 1957.
35,646
8.
E.M. Southern
"Detection of specific sequences among DNA fragments separated by gel electrophoresis,"_/
Mol Biol,
98:503
-
17, 1975.
31,273
9.
J.M. Chirgwin et
al.
"Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease,"
Biochemistry,
18:5294
-
9, 1979.
23,909
10.
K. Weber, M.
Osborn
"Reliability of molecular weight determinations by dodecyl sulfate
-
polyacrylamide gel electrophoresis,"J
Biol
Chem,
244:4406
-
12, 1969.
23,619
With better technology…
Biotech Business
Factor VIII
-
hemophilia
Etanercept
-
arthritis
Follicle stimulating protein
-
infertility
Human Insulin
-
diabetes
Erythropoietin
-
anemia
TPA
–
heart attacks and strokes
http://bioweb.uwlax.edu/GenWeb/Molecular/Molecular_Biology_Syllabus/tPA/tPA_image/tpa_image.htm
Enter the password to open this PDF file:
File name:
-
File size:
-
Title:
-
Author:
-
Subject:
-
Keywords:
-
Creation Date:
-
Modification Date:
-
Creator:
-
PDF Producer:
-
PDF Version:
-
Page Count:
-
Preparing document for printing…
0%
Σχόλια 0
Συνδεθείτε για να κοινοποιήσετε σχόλιο