Biotech Instrumentation

Biotech
Methods

BITC
1402
Dr. Larry Loomis
-
Price
Spring 2008

Who am I?

Dr. Larry

Labman

Biotech is a lab science

What is Biotechnology?

What is Biotechnology?


Oldest forms of
biotechnology
techniques for making
yogurt and cheese
(with bacteria); bread,
beer and wine
(yeast).

What is Biotechnology?


The use of living
organisms or parts
or organisms to
make a useful
product or as part
of an industrial or
technical process

What is Biotechnology?

•
Newer products of the
biotech industry are
found in many fields,
including the “5 F’s”:

•
Food

•
Fiber

•
Fuel

•
Filtration

•
Pharmaceuticals

We Use Instruments
-

Small

•
Hot plate/stirrers

•
Pipettors

•
pH meters

•
Microscopes

•
Minifuges

•
Balances

And
Large (next semester)

•
Spectrophotometers

•
Electrophoresis rigs

•
HPLC’s

•
Mass Spec’s

•
DNA sequencers

•
nmr
, X
-
ray etc.

TOP PAPERS PUBLISHED





OF ALL TIME



1.

O.H. Lowry et al.

"Protein measurement with the Folin phenol reagent, "J
Biol Chem,
193:265
-
75, 1951.

293,328

2.

U.K. Laemmli

"Cleavage of structural proteins during assembly of head of bacteriophage T
4
,"
Nature,
227:680
-
5, 1970.

192,022

3.

M.M. Bradford

"Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein
-
dye binding,"
Anal Biochem,
72:248
-
54, 1976.

120,179

4.

F. Sanger et al.

"DMA sequencing with chain
-
terminating inhibitors,"
Proc Natl Acad Sci,
74:5463
-
7, 1977.

63,909

5.

P.Chomczyski,N
.Sacchi

"Single
-
step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction,"
Anal
Biochem,
162:156
-
9, 1987.

55,987

6.

H. Towbin etal.

"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some
applications,"
Proc Natl Acad Sci,
76:4350
-
4, 1979.

48,671

7.

J. Folchetal.

"A simple method for the isolation and purification of total lipides from animal tissue, "J
Biol Chem,
226:497
-
509, 1957.

35,646

8.

E.M. Southern

"Detection of specific sequences among DNA fragments separated by gel electrophoresis,"_/
Mol Biol,
98:503
-
17, 1975.

31,273

9.

J.M. Chirgwin et
al.

"Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease,"
Biochemistry,
18:5294
-
9, 1979.

23,909

10.

K. Weber, M.
Osborn

"Reliability of molecular weight determinations by dodecyl sulfate
-
polyacrylamide gel electrophoresis,"J
Biol
Chem,
244:4406
-
12, 1969.

23,619

With better technology…

Biotech Business

Factor VIII
-

hemophilia

Etanercept

-

arthritis

Follicle stimulating protein
-

infertility

Human Insulin
-

diabetes

Erythropoietin
-

anemia

TPA
–

heart attacks and strokes

http://bioweb.uwlax.edu/GenWeb/Molecular/Molecular_Biology_Syllabus/tPA/tPA_image/tpa_image.htm