M.Sc. MARINE BIOTECHNOLOGY
PAPER 1.1: Fundamentals of Oceanography
ature, light, transparency, tur
pressure, water currents, tides and waves.
Chemical properties: salinity, dissolved oxygen, pH, n
on of seawater; estuarine and brackish waters.
Law of the sea
Remote sensing applications in monitoring ocean habitats and coastal
1. O.Kinee(ed)Marine Ecology Vols I,II& VJOhn Wiley and Sons.
PAPER 1.2: Principles of Biochemistry
Covalent bond, coordinate bond, hydrogen bond, ionic
bond, vanderwall’s forces, hydrophobic & hydrophilic interactions.
Structure and a chemistry of biomolecules
carbohydrates of fish and shellfish(shrimp &
Amino acids and proteins;
proteins of fish and shellfish
Nucleic acids and porphhyrins
Chemistry and properties of vitamins and hormones.
Biological membranes & transport of biomo
er, Biochemistry, W.H. Freeman & Co
2. A.L. Lehninger, Principles of Biochemistry, CBS Publishers
PAPER 1.3 Cell Biology and Genetics
Cell : ultra Structure, nutrient transport, cytoskeleton organizatio
of prolutin translocation
Cell nucleus : chromosomes, nucleic acids, eukaryotic chromosomes,
heterchromatin, molecular components packing & organization;
Cell division Chromosomal & extra chromosomal, mole
cular basis of
inheritance ; Mendelian principles of inheritance; allelism &
complementation; sex linked inheritance ; quantitative genetics ;
Gene structure & function: transduction, conjugation DNA as genetic
material; easy phages to study T
1,T2,T3 and T7.
D.Frifielder: Microbial genetics
B.Lewin: Genes VII.John Wiley
Arberts et al: Molecular Biology of cells
H.Lodish, A.Berk, S.L. Zipursky, P.Matsudaria, D.Baltimore and
J.Damell. Molecular Cell Biology, W.H.Freeman & Company,
R.F. Weaver. Molecular Biology.W.C.V.McGraw Hill, Dubeque,
S.R.Malog. Microbial Genetics
PAPER1.4: Marine Microbiology
General classification and taxonomy of microorganisms
Distribution of microorganisms in the marine habitats
micrometazoans, microalgae and fungi; culture techniques
Bacteria: Ultra structure, growth & life cycle; culture
Methods of Isolation and Identification of Marine Microbes.
Methods of sterilization, inoculation techniques using different media
methods of isolation of bacteria and fungi.
Pelczar, Chann and Kreig : Microbiology (5
Edition).McGraw Hill Inc.
Salle A.J: Fundamental principles of Bacteriology Tata McGraw Hill
Stainer RT et al : General Microbi
ology. Mac Millan
Madigan,Martinko and parker : Brock biology of microorganisms. 9
Dimmock and primrose : Introduction to viruses. 2
Biswas and Biswas : An introduction to viruses. 2
Luria, Darnell, Baltimore and Campbell : G
eneral Virology, Wiley, New
Ross : Introduction to Microbiology
Tauro, Kapoor and Yadav : An introduction to Microbiology
Cappuccino and Sherman : Microbiology: A Laboratory Manual
PAPER 1.5 : Enzymology
Nomenclature, classification of
Enzyme structure, properties
Enzyme specificity; factors affecting enzyme action
Mechanism of enzyme action activators & inhibitors; characterization of
enzyme complex; single and multi
ase, glutamine synthetase, hemoglobin and myoglobin
Membrane bound enzymes : extraction, purification and assay.
Boyer, P.D : The enzymes Vols. 1&2. A.P. Freeman
Fersht, A.R : Enzyme structure & Mechanism A.P Freeman
Palmer T : Enzymes : Horwood pu
PAPER 2.1: Marine Biology
Classification of marine habitats
Composition & distribution of Marine plants : seaweeds &
Zooplankton: collection composition & distribution
ton : collection, composition & distribution of important finfish &
shell fish resources.
Marine reptiles & mammals
Benthos : collection, composition & distribution of benthic organisms.
Mangroves and coral reef ecosystems : General characters
Impacts of man on marine habitats : Marine pollution & Over
K.H.Mann : Ecology of coastal waters
RSK Barners & R.N Huges : Introduction to Marine Ecology
PAPER 2.2 : Principles of Biochemistry
Lipids: Biosynthesis and oxidation fatty acids; Ketone bodies.
Biosynthesis of amino acids, nucleotides, and their regulation.
and general properties.
H.Stryer: Biochemistry. W.H.Freeman &Co.
2.B. Lewin: Genes VII. John Wiley
3. Voet & Voet : Biochemistry (2
4.Martin, Mayer &Rodwell : Harpers’ review of Biochemistry.
5. Plummer : An Introdu
ction to Biochemistry.
PAPER 2.3 Molecular Biology
DNA replication : Principles in eukaryotes & prokaryotes, enzymes
involved in replication, DNA polymerases, RNA primer, Primosome
ssb proteins; discontinuous synthesis.
Transcription : DNA
RNA hybrid; enzymes involved in RNA synthesis;
Reverse transcription; genetic manipulations; RNA processing; Nuclear
as catalyst: ribozyme.
_ Regulation systems :
abolite repression and activation;
Protein Synthesis : translation factors : mRNA, tRNA & rRNA
Structure and function ; genetic code.
Genetic Recombination : homogenus & heterogenus genetic
recombinations; DNA repair
utations : Types of mutations, detection, isolation & reversion.
Molecular basis of mutations, consequences and utilizations of
1. B. Lewin: Genes VII. John Wiley
2. Watson et al : Molecular Biology of Gene. Benj. Cumm.
PAPER 2.4: Microbial Technology
Role of microorganisms in sea : recycling of nutrients.
Estuarine and mangrove microbiology
Microbial degradation of carbohydrates, proteins and lipids
Fermentation and bioconversion by microorganisms
l principles of bioreactors
Control of microorganisms : Physical, chemical and chemotherapeutic
Microalgal biotechnology : Single cell proteins :
H H Rehm & G. Reed : Biotechnology Vol. IV
G. Reed et
al : Industrial Microbiology. C.B.S. Publishers
PAPER 2.5 : Enzyme Technology
Cell & enzyme reactors; fusion and diffusion control.
protein interactions and effect of fluidity on enzyme activity
d in nucleic acid modification: methylases, dam
methylases, DNAses , RNAses , gyrases , topoisomerases &
Enzyme Kinetics : Steady State Kinetics ; single and multi substrate
reactions . Competetive, non
competetive and uncompetitive enzyme
Immobilization of enzymes : biosensors & modifications ; biological
Synthetic enzymes; problems & perspectives of co
Enzymes of industrial and diagnostic importance.
1.Siseman A. ( ed ) : Hand Book of enzyme technology
2. Trevan M.D. : Immobilized Enzymes. Wiley & Sons
3. Plowman K. M. : Enzyme Kinetics. McGraw Hill
M.Sc Marine Biotechnology
of gene, introduction to plasmids, cosmids and lamda carrier DNA
called Vectors. Principles of plasmid preparation. Genetic types of various
Restriction Enzymes : Type I, II, III. Klenov Fragm
ent, commonly used
restriction enzymes resulting blunt ends and cohesive ends.
Stringent and relaxed plasmids.
Bacterial resistance to antibiotics; submarine gel electrophoresis and blotting
Introduction to foreign DNA into plasmids, cosmids an
d lamda DNA.
Principles of using linkers and adaptors
Detection of cloned genes by transformation experiments.
Introducing cDNA as probe; kinasing experiments and incorporating 32
W. Old & Primrose :
of gene manipulatio
ns S B Univ. Press.
Lewin B. Genes
V . John Wiley.
PAPER 3.2 : Principles of Immunology
Immunity : History, scope and types of immunity.
Antigens : Types and properties
Immunoglobulins : Structure and properties.
: Major histocompatibility gene complex.
Genetic basis of antibody diversity.
Molecular basis of B & T cells
Mechanisms of cell mediated cytotoxicity.
Compliment proteins : types and pathways.
Cytokines : types and functions
Invertebrate immune system.
1. L. Hood et al. Immunology. Benj. Cumur. Press
2. W. E. Paul. Fundamentals of immunology. Raven Press
PAPER 3.3. Marine Living Resources
Marine Plant Resources: Phytoplankton, seaweeds, sea grasses, mangroves
their utilization and rational explo
Marine animal resources : crustaceans, mollusks and finfish resources
utilization and rational explo
Culture of live plant feeds; sea ranching of algae.
Culture of live animal feeds; ro
brine shrimp (
ranching of economically important marine animals.
Jhingran V. G. Fish and Fisheries of India (2
Prossar C.L. Comparative animal physiology
Kinne O. Marine Ecology , Vol. 3 , Part 3.
Paper 3.4: Bioactive Marne Natural Products
Bioactivities of Marine Natural Products. An introduction to the of marine natural
tumor, repellent, anti
parasitic, Microbial, antifouling.
Commercial potential and de
velopment of marine natural products.
Chitosan as a biomaterial.
Algae products: carrageenan, algin, B
carotene and vitamins.
Marine microorganisms as a new biomaterial resources (marine microalgae, fungi
Dinoflagellates as a source of bioac
tive compounds: A brief introduction of isolation,
chemistry and pharmacology of saxitoxin, brevitoxin and cigatoxn Tunicromes.
Isolation techniques: Liquid
Liquid extraction, membrane separation methods and
Chromatography (TLC, HPLC) and conventional te
Characterization techniques: IR, UV, NMR, and mass spectra.
David H.Attway & Oskar R.Zabosky: Marine Biotechnology:
Plenum Press, 1993.
P.J.Scheuer: Marine. Natural Products, Volume 1 & 2 (1978). Volume (1
81) Academic Press.
Paper 3.5: Marine Pollution
Marine Pollution: Definition by GESAMP, major sources of pollution, dynamics,
transport paths and agents.
Toxicology: Lethal and sub lethal effects of pollutants on marine organisms,
tion of toxicity tolerance, bioassay.
Enzymatic removal of hazardous organic substances from aqueous effluents.
Sewage: Domestic, Industrial, agricultural and aquacultural discharges, their
composition and fate in the marine environment, toxicity and treat
sewage disposal system.
Environmental Impact Assessment Methods of aquaculture activities.
Oil pollution: Sources and fate of oil, composition and toxicity of oil, biological effects
Thermal and radioactive pollutants:
Source and characteristics, strategies for
disposal of RNA and Heated effluents, biological effects and alternative uses of
waste dumping, mining and dredging operations, their effects on the organisms and
. Nielsen: Tropical Pollution, 1982.
A.M.Chakravarthy Biodegradation and detoxification of Environmental
pollutants, CRC Press, 1928.
O.Kinne: Marine Ecology, Vol.V.Ocean Management 3&4, John Wiley &
Johnston R. (Ed.) : Marine Pollutio
n, Academic Press, 1976.
Patin. S.A.: Pollution and Biological resources of the Oceans Butterworth &
Co. Ltd., 1982.
Ruive M. (Ed.) : Marine Pollution and Fishing News Sea Life, 1972.
Venugopalan, V.K.: Pollution and Toxicology, CAS in Marine Biol
Rita Colwell (Ed.): Biotechnology in Marine Sciences, Academic Press, 1981.
Prakesh P.: Textbook of Marine Pollution.
Gupta, P.K.: Introduction to Biotechnology.
M.Sc. Marine Biotechnology
Paper 4.1 : Genetic Engineer
Construction of cDNA libraries, Nick translation, probes using oligos, RNA, DNA and
Restriction mapping: hybridization: radioactive and nonradioactive. Introduction to
M13 phage DNA for MCS, directional & non
site specific mutagenesis study gene function. Nested Deletion
Transfection, Transferring of genes into cells.
Construction of Genomic DNA libraries.
DNA Fingerprinting and Foot printing and chromosomal wa
DNA sequencing; Sanger’s Dideoxy sequencing and Maxam & Gilbat sequencing.
Automated DNA sequencing using fluorescence.
Analysis of cloned genes.
PCR basic principles.
Introduction to oncogenes & oncogenesis
A molecular detail.
Biological risks, ethical, economical and legal issues.
W.Old & Primrose: Principles of gene manipulation SB Univ. Press.
Lewin.B: GENES V, John Wiley.
Paper 4.2 : Applications of Immunology
Immunological techniques: Immunodiffusion, Immunoelectropheres
Immunoflourescene, ELISA and RIA.
Production of Monoclonal antibodies.
Hypersenstivity reactions: Antibody
mediated and Cell
Auto immunity m
Immunotherapy techniques: Vaccines types and their preparation.
Immunology of infectious diseases.
Hood et al.: Immunology, Benn
. Cumm. Press.
Riott: Essentials o
Golub: Immunology a synthesis, Sinave
Principles and Practices
Paper 4.3 : Application of Biotechnology
cations in Aquaculture: Chromosomal manipulations:
gynogenesis, androgenesis, polyploidy and transgenics.
Hybridization: Production of new strains for broodstock improvement, hormonal
control and induced breeding.
Preparation of Synthetic feeds: Compositio
n, formulation and processing.
Tissue Culture: Culture techniques of plant (marine algae and mangroves) and
animal (crustaceans, molluscs & fishes
) tissues, preservation of germ
eservation of tissue c
ltures, IN VITRO fertilization technique.
Gene manipulations to improve strains: Industrial applications of tissue culture
Diseases in marine organisms: Application of biotechniques in disease diagnosis,
prevention and control
gene probes and their industrial applications.
R.R.Colwell (ed), Biotechnology of Marine Science, 1982.
R.R.Colwell et. al (eds) Biotechnology of Marine polysaccharides, 1985.
Paper 4.4. Bioactive Marine Natural Products
Bioactive marine natural products: Anti
, tumour promoting, anti
inflammatory, cytotoxic, anti
neoplastic and analgesic compounds.
Nitrogen containing marine natural products: Amides, (Symbioramide,
A), Tyrosin based metabolites (Aeroplisinin
C) Imadezoles (Girolline), Pyridines (Theonelladines),
Peptides (didemnins, Dolastatins), ara
A, nucleosides (toyocamnins), swinholide
Non Nitrogenous Bioactive Compounds: polyketides (Dysidazirine, ficulinic
nd B, Duryne, Aliphatic esters, peroxides), prostanoides (clavulone
1, chloro, bromo and iodo vulones).
Polyethers (hemibrevitoxin B, lokadaicacid), macrolides (amphidinilide .A.
swinholide A, bryostatins) Terpenes, laplysiterpenoid, geran
Averol and Averone, curcuphenol, cembranoides, brantheines V&Z,
oxygenated sterols and saponins (sarasinoside A, Xeniasterol C, Imbricatosides A
and B, cephalostatins).
David H.Attway & Oskar R.Zabosky: Marine Biotechnology, Vo
plenum press 1993.
P.J.Scheuer: Marine Natural Products, Volume 1&2 (1978) Volume (1980,
81), Academic Press.
Paper 4.5: Environmental Monitoring and Biodeterloration
Global environmental monitoring methods: Stat
us and objectives, limitations for
monitoring critical pollutants.
Role of biotechnology in environmental pollution control: Indicator organisms, Test
organisms, Monitoring organisms, Enzymes.
Coastal developmental activities
d Macro fouling, corrosion of metals and alloys in the sea, effects of bio
fouling and bio deterioration on marine structures.
Protection methods against corrosion and fouling Application of biotechnology in
controlling the bio deterioration of wood and sy
nthetic substances in the sea.
Red tides: Cause character and effects on the organisms of Marine environment
S.E. Nielsen: Tropical Pollution, 1982.
Chakravarthy: Biodegradation and detoxification of Environmental
nts, CRC Press, 1928.
Kinne: Marine Ecology, Vol.V. Ocean Management 3&4 John Wiley &
Johnston R. (Ed.) : Marine Pollution, Academic Press, 1976.
Patin S.A.: Pollution and Biological resources of the Oceans Butterworth &
Ruive M. (Ed.) : Marine Pollution and Fishing News Sea Life 1972.
Venugopalan, V.K. : Pollution and Toxicology, CAS in Marine Biology, 1991.
Rita Colwell (Ed): Biotechnology in Marine Sciences, Academic Press, 1981.
Prakash P.: Textbook
of Marine Pollution.
Gupta, P.K. : Introduction to Biotechnology.