PS 454/554 Plant Biotechniques - Department of Plant Sciences

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3 Δεκ 2012 (πριν από 4 χρόνια και 8 μήνες)

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PS 454/554 Plant Biotechniques


Instructor: Janice Zale

Office: 346 PBB

Lab: 343 PBB

Phone Office: 974
-
8596

Phone Lab: 974
-
8834

Email:
jzale@utk.edu

Office Hours: drop by or schedule an ap
pointment



Course description:


454/554 Plant Biotechniques (3). Lectures will discuss recombinant DNA technology,
molecular assisted breeding of economically important crops, gene cloning and
transformation technologies. Examples will be given of food

and ornamental crops,
pharmaceuticals, and renewable sources of energy produced using biotechnology as well
as potential risks of this technology. Labs will include electrophoresis, tissue culture,
plasmid preps, genomic DNA preps, plant transformation a
nd genomic techniques.
Prereq: Biology 240 or Plant Sciences 353 or consent of the instructor. 1.5 hours of
lecture and 3 hours of lab

or equivalent
.


Obectives
: To educate students in Plant Biotechnology particularly
as
it relates to
agriculture.


Req
uired
Textbook:

Analysis of Genes and Genomes
.
2004
.

Richard J Reece.

John
Wiley and Sons Ltd.,West Sussex, England.


Handouts will
also
be supplied.


Lecture and Lab Lecture
:



most
Tuesday
s


8:10 A.M.
-
10
:55

A.M.
, Dec 1


Lab
s
:





most
T
hursday
s

9:
30 A.M.
-
12:25 P.M.
, Nov 22 and Nov 29



Attendance at all class
es

and l
aboratory sessions is encouraged
. S
ome lab
oratory
sessions
are contingent on
results from t
he previous lab. Absences due to
extenuating
circumstances

will be allowed after consultatio
n with the instructor.

Without an excused
absence, a score of zero may be given for exams and/or assignments.


Lab
books/assignments

handed in late will be penalized.



University’s Honor Statement
:

“An essential feature of the University of Tennessee is

a commitment to maintaining an
atmosphere of intellectual integrity and academic honesty. As a student of the University,
I pledge that I will neither knowingly give nor receive any inappropriate assistance in
academic work, thus affirming my own personal

commitment to honor and integrity.”






Evaluation and Grades


Mid
-
term exam

150 pts

Final exam

175 pts

Lab book


75 pts

2

Quizzes 100
pts




500 pts total


Note: Graduate students will
have to submit an essay
after

consultation with instructor
and will
be marked separately from undergraduates
.




Grading (%)



92

=

A


460 pts









8
6

=

B
+


430

pts






80

=

B

400 pts




7
6

=

C+


380 pts


75

= C

375 pts




6
9


=

D

345 pts





59

=
F


295 p
t
s


Expected Outcome
:


This class will aid in equipping students with the skills to work in a Life Science
Laboratory, the field of Biotechnology, or continue research at the graduate level.





















Lecture/Lab Schedule


Week



Lecture


Book
Chapter


Lab Lecture


Lab









Aug 25

Overview
of Class

Review

Making Solutions, Sterile
Technique
, Pipetting
, Pouring Plates

Centrifugation


Aug 30


Review

Macromolecules



1
, 2.4



Sept 1

DNA Quantification

Bacterial Transformatio
n

Determining Transformation
Efficiency

of
Competent Cells


Sept 6


Restriction Enzymes

Modifying Enzymes

Gel Electrophoresis


2


Sept 8

Plasmid Minipreps

DNA Quantity/Quality Check


Sept 13


Vectors

Selectable Markers

Recombinant DNA
Technology

3

&
h
andouts


Sept 15

Restriction Digests

Gel Electrophoresis

Gel extraction


Sept 20


PCR and its
Applications


4 &
handouts


Sept 22

Directional Cloning
,

Bacterial Transformation


B
lue/White

Selection


Sept 27


PCR and its
Applications


Sept 29

Miniprep
cultures and check for
recombinants


Oct 4


Gene Cloning

5


Oct 6

PCR

Run PCR Products


Oct 11


Midterm



Fall break



Oct 18


Molecular Markers in
Mapping and
Selection


handouts


Oct 20

Genomic DNA Preps

RNA preps (handouts)


Oct 25


Creating Mutatio
ns in
Bacteria and Plants

7 and
handout


Oct 27

Southern
Blotting

Northern Blotting (handout)

Hybridization

(handout)



Nov 1


Plant Transformation

Applications

Ethics


11 and
handouts


Nov 3

Gene Gun
-
Transient Expression of Lc/C1
in dissected wheat scut
ellum


In Planta
Transformation


Nov 8


Genomics

9 and
handouts


Nov 10

Databases

Blast Searches

Sequence Alignment in Computer Lab


Nov 15


Post
-
Genom
ic

Analysis

10 and
handouts


Nov 17

Microarray

-
RNA labelling


Nov 22


Lab

Microarray
-

Purification o
f
labelled products and
h
ybridization

handouts

Nov 24

Thanksgiving



Nov 29



Lab

Microarray
-
Slide
washing and scanning,
Brief Intro to Data
Analysis

handouts


Dec 1

Applications of Biotechnology

Biotechnology
-

Risks

And Benefits



Dec 6

Last Class


Intellectual Property
Rights
,
PVP

Laboratory Books
Submitted for Grades





Final Exams weeks of Dec 9, 12, 13 and 15