Syllabus for B.Tech(Biotechnology) Second Year - WBUT

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Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


1
2
nd
Year - Semester III
A. THEORY
Contact Hours/Week

S/L Code Theory
L
T
P
Total
Cr. Points
1
HU 301
Values&EthicsinProfession
3
0
0
3
3
2 CH 302 Chemistry 2 3 1 0 4 4
3
CH 301
BasicEnvironmental
Engineering&Elementary
Biology
3
0
0
3
3
4
BT 301
ThermodynamicsandKinetics
3
0
0
3
3
5
BT 302
Biochemistry
3
1
0
4
4
6
BT 303
Microbiology
3
1
0
4
4
Total of Theory
21 21
B. PRACTICAL
7 CH 392 Chemistry 2Lab 0 0 3 3 2
8
CH 391
BasicEnvironmentand
ElementaryBiology

Lab
0
0
3
3
2
9
BT 392

BiochemistryLab
0
0
3
3
2
10
BT 393
MicrobiologyLab
0
0
3
3
2
Total of Practical
12 8
Total of Semester 33 29


2
nd
Year - Semester IV

A. THEORY
Contact Hours/Week

Code Field Theory
L
T
P
Total
Cr. Points
1
M(CS) 401
NumericalMethods
2
0
0
2
2
2

IndustrialStoichiometry
3
1
0
4
4
3

TransferOperationI
3
0
0
3
3
4
BT 401
MolecularBiology
3
1
0
4
4
5
BT402
IndustrialMicrobiologyand
EnzymeTechnology
3
1
0
4
4
Total of Theory 17 17
B. PRACTICAL
6
HU 481
Communicationskill&Report
writing
0
0
3
3
2
7
M(CS) 491
NumericalMethods
0
0
2
2
1
8

TransferOperationILab
0
0
3
3
2
9
10
BT 491
BT 492
MolecularBiologyLab
FermentationTechnologyLab
0
0
0
0
3
3
3
3
2
3
Total of Practical 14 9
Total of Semester
31 26

Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


2

Detailed Syllabus
Note1:Therewillbeonecompulsoryobjectivetypequestioncomprisingtennumbersspreadovertheentiresyllabus
andeachcarryingonemark.
Note2:Twoquestionsaretobesetfromeachmoduleoutofwhichfivequestionsaretobeansweredtakingatleast1
fromeachmodule.Allquestionscarryequalmarks.

Semester III

VALUES & ETHICS IN PROFESSION
H301
Contracts:3L
Credits- 3
Science,TechnologyandEngineeringasknowledgeandasSocialandProfessionalActivities
Effects of Technological Growth:
RapidTechnologicalgrowthanddepletionofresources,ReportsoftheClubofRome.Limitsofgrowth:sustainable
development
EnergyCrisis:RenewableEnergyResources
Environmentaldegradationandpollution.Eco friendlyTechnologies.EnvironmentalRegulations,Environmental
Ethics
AppropriateTechnologyMovementofSchumacher;laterdevelopments
Technologyanddevelopingnotions.ProblemsofTechnologytransfer,Technologyassessmentimpactanalysis.
HumanOperatorinEngineeringprojectsandindustries.Problemsofman,machine,interaction,Impactofassembly
lineandautomation.HumancenteredTechnology.
Ethics of Profession:
Engineeringprofession:EthicalissuesinEngineeringpractice,Conflictsbetweenbusinessdemandsandprofessional
ideals.SocialandethicalresponsibilitiesofTechnologists.Codesofprofessionalethics.Whistleblowingandbeyond,
Casestudies.
Profession and Human Values:
ValuesCrisisincontemporarysociety
Natureofvalues:ValueSpectrumofagoodlife
Psychologicalvalues:Integratedpersonality;mentalhealth
Societalvalues:Themodernsearchforagoodsociety,justice,democracy,secularism,ruleoflaw,valuesinIndian
Constitution.
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


3
Aestheticvalues:Perceptionandenjoymentofbeauty,simplicity,clarity
Moralandethicalvalues:Natureofmoraljudgements;canonsofethics;ethicsofvirtue;ethicsofduty;ethicsof
responsibility.
Books:

1. StephenHUnger,ControllingTechnology:EthicsandtheResponsibleEngineers,JohnWiley&Sons,NewYork
1994(2
nd
Ed)
2. DeborahJohnson,EthicalIssuesinEngineering,PrenticeHall,EnglewoodCliffs,NewJersey1991.
3. ANTripathi,HumanvaluesintheEngineeringProfession,MonographpublishedbyIIM,Calcutta1996.
Chemistry-2
CH-302
Contracts:3L+!T
Credits- 4
Module I
Bio Molecules          6
Carbohydrates – Classification: aldoses and ketoses; monosacchar ides (glucose and fructose), constituent
monosaccharidesofoligosacchorides(sucrose,lactose,maltose)andpolysaccharides(starch,cellulose,glycogen).
Proteins – Elementary Idea of – amino acids, peptide bond, polypeptides; proteins: primary, secondary, tertiary and
quaternarystructure(qualitativeideaonly),denaturationofproteins,enzymes.
Vitamins –Classificationandfunctions.
Nucleic acids–ChemicalconstitutionofDNAandRNA.Biologicalfunctionsofnucleicacids.

Module II
Molecular Spectroscopy 7
UV Spectra

Electronic transition (σ σ*, n σ*, π π* and n π*), relative positions of λmax considering conjugative effect, steric
effect,solventeffect,redshift(bathochromicshift),blueshift(hypsochromicshift),hyperchromiceffect,hypochromic
effect(typicalexamples).
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


4
IR Spectra
Modesofmolecularvibrations,applicationofHooke’slaw,characteristicstretchingfrequenciesofO H,N H,C H,C
D,C=C,C=N,C=Ofunctions;factorseffectingstretchingfrequencies(H bonding,masseffect,electronicfactors,bond
multiplicity,ringsize).
PMR Spectra
Nuclear spin, NMR active nuclei, principle of proton magnetic resonance, equivalent and non equivalent protons,
chemical shift δ), shielding/deshielding of protons, up field and down field shifts. NMR peak area (integration),
diamagneticanisotropy,relativepeakpositions of differentkinds of protons(alkylhalides,olefins,alkynes,aldehyde
H),substitutedbenzenes(toluene,anisole,nitrobenzene,halobenzene,dinitrobenzenes,chloronitrobenzene),firstorder
coupling(splittingofthesignals:ordinaryethanol,bromoehane,dibromoehanes),couplingconstants.
Module III
Polymer Blends & Composites 6 
Difference between blends and composites, their significance, choice of polymers for blending, blend miscibility
miscible and immiscible blends, thermodynamics, phase morphology, polymer alloys, polymer eutectics, plastic
plastic,rubber plasticandrubber rubberblends.
FRP,particulate,longandshortfibrereinforcedcomposites.
Adhesive 3
Introduction,Classificationofadhesive,adhesiveaction,adhesivestrength,bondingofadhesive,varioustypeoffailure
ofadhesivejoint.
Protective coatings 5
Introduction, Types of protective coatings (Metallic Coatings  Anodic Coatings, Galvanization, Cathodic Coatings 
Tinning,MetalCladding,ElectroplatingEx:ChromiumPlating,Metalspraying,cementation sheradizing,colourizing,
chromizing)
SurfacePreparation:solventcleaning,alkalicleaning,Picklingandetching,sandblasting,mechanicalcleaning.
ChemicalConversionCoatings:(1)Phosphate(2)Chromate(3)AnodizedCoatings.
Organic coatings, paints, formulation of paints, failure of paints, varnishes, enamels, lacquers, Emulsion paint, self
healingpaint.

Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


5
Module IV
General treatment of reaction mechanisms 7
Ionicandradicalreactions;heterolyticand,homolyticbondcleavage
Reactive intermediates:carbocations(cabeniumandcarboniumions),carbanions,carbon
radicals, carbenes –structure using orbitalpicture,electrophilic/nucleophilic behaviour, stability, generation andfate.
Reaction kinetics:transitionstatetheory,rateconstandfreeenergyofactivation,freeenergyprofilesforonestepand
twostepreactions,
Nucleophilic substitution reactions  SN1, SN2, SNi mechanisms. Effect of substrate structure, nucleophiles and
mediumonreactivityandmechanism;neighboringgroupparticipations.
Elimination Reactions- E1,E2,andE1cBmechanisms.SaytzeffandHofmannrules.
Eliminationvssubstitutionreaction.
ElectrophilicandActivatedNucleophilicsubstitutionreactionsofBenzene(Nitration,sulphonation,Halogenationand
FriedelCraftreactions)
Module V
Photochemistry 3
Lambert’slawandBeer’sLaw,Lawsofphotochemistry,Photochemicalprocesses,photosensitization,photochemical
equilibrium,fluorescence,phosphorescenceandchemiluminiscence.
Catalysis 3
Homogeneous,heterogeneous,andenzymatic.Conceptsofpromotors,inhibitors,andpoisioners.

Basic Environmental Engineering and Elementary Biology
CH-301


L-T-P = 3-0-0 
Atleast30Hrs/Sem
General
Basicideasofenvironment,basicconcepts,man,society&environment,theirinterrelationship.  
      1L
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


6
Mathematics of population growth and associated problems, Importance of population study in environmental
engineering, definition of resource, types of resource, renewable, non renewable, potentially renewable, effect of
excessiveusevis à vispopulationgrowth,SustainableDevelopment.     
 2L
Materials balance: Steady state conservation system, steady state system with non conservative pollutants, step
function.      1L
Environmental degradation: Natural environmental Hazards like Flood, earthquake, Landslide causes, effects and
control/management; Anthropogenic degradation like Acid rain cause, effects and control. Nature and scope of
EnvironmentalScienceandEngineering.        
  2L
Ecology
Elements of ecology: System, open system, closed system, definition of ecology, species, population, community,
definitionofecosystem componentstypesandfunction. 1L
Structureandfunctionofthefollowingecosystem:Forestecosystem,Grasslandecosystem,Desertecosystem,Aquatic
ecosystems, Mangroveecosystem(special referenceto Sundarban); Foodchain[definition and oneexample ofeach
foodchain],Foodweb.  2L
BiogeochemicalCycle definition,significance,flowchartofdifferentcycleswithonlyelementaryreaction[Oxygen,
carbon,Nitrogen,Phosphate,Sulphur].   1L
   Biodiversity  types, importance, Endemic species, Biodiversity Hot spot, Threats to
biodiversity,Conservationofbiodiversity.      2L
Air pollution and control
AtmosphericComposition:Troposphere,Stratosphere,Mesosphere,Thermosphere,TropopauseandMesopause.
       1L
Energy balance: Conductive and Convective heat transfer, radiation heat transfer, simple global temperature model
[Earthasablackbody,earthasalbedo],Problems.  1L
Greenhouseeffects:Definition,impactofgreenhousegasesontheglobalclimateandconsequentlyonseawaterlevel,
agriculture and marine food.Global warming and its consequence, Control of Global warming. Earth’s heat budget.
1L
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


7
Lapserate:AmbientlapserateAdiabaticlapserate,atmosphericstability,temperatureinversion(radiationinversion).
        2L
Atmospheric dispersion: Maximum mixing depth, ventilation coefficient, effective stack height, smokestack plumes
andGaussianplumemodel.     2L
Definitionofpollutantsandcontaminants,Primaryandsecondarypollutants:emissionstandard,criteriapollutant.
Sources and effect of  different air pollutants  Suspended particulate matter, oxides of carbon, oxides of nitrogen,
oxidesofsulphur,particulate,PAN.     2L
Smog,PhotochemicalsmogandLondonsmog.
Depletion Ozone layer: CFC, destruction of ozone layer by CFC, impact of other green house gases, effect of ozone
modification.       1L
Standards and control measures: Industrial, commercial and residential air quality standard, control measure (ESP.
cycloneseparator,baghouse,catalyticconverter,scrubber(ventury),Statementwithbriefreference). 
      1L
Water Pollution and Control
Hydrosphere,HydrologicalcycleandNaturalwater.
Pollutantsofwater,theiroriginandeffects:Oxygendemandingwastes,pathogens,nutrients,Salts,thermalapplication,
heavymetals,pesticides,volatileorganiccompounds. 2L
River/Lake/groundwaterpollution:River:DO,5dayBODtest,SeededBODtest,BODreactionrateconstants,Effect
ofoxygendemandingwastesonriver[deoxygenation,reaeration],COD,Oil,Greases,pH.  
    2L
Lake:Eutrophication[Definition,sourceandeffect].     1L
Groundwater:Aquifers,hydraulicgradient,groundwaterflow(Definitiononly) 1L
Standardandcontrol:Wastewaterstandard[BOD,COD,Oil,Grease],
Water Treatment system [coagulation and flocculation, sedimentation and filtration, disinfection, hardness and
alkalinity,softening]
Waste water treatment system, primary and secondary treatments [Trickling filters, rotating biological contractor,
Activatedsludge,sludgetreatment,oxidationponds]tertiarytreatmentdefinition.   
      2L
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


8

Waterpollutionduetothetoxicelementsandtheirbiochemicaleffects:Lead,Mercury,Cadmium,andArsenic
        1L
          
Land Pollution
Lithosphere;Internalstructureofearth,rockandsoil    1L
Solid Waste: Municipal, industrial, commercial, agricultural, domestic, pathological and hazardous solid wastes;
Recoveryanddisposalmethod Opendumping,Landfilling,incineration,composting,recycling.
Solidwastemanagementandcontrol(hazardousandbiomedicalwaste).   2L

Noise Pollution
Definitionofnoise,effectofnoisepollution,noiseclassification[Transportnoise,occupationalnoise,neighbourhood
noise]      1L
Definition of noise frequency, noise pressure, noise intensity, noise threshold limit value, equivalent noise level,
10
(18hr Index)
L,
n
Ld
.
Noisepollutioncontrol.         1L
Environmental Management:
Environmental impact assessment, Environmental Audit, Environmental laws and protection act of India, Different
internationalenvironmentaltreaty/agreement/protocol.  2L

References/Books
1. Masters, G. M., “Introduction to Environmental Engineering and Science”, Prentice Hall of India Pvt. Ltd.,
1991.
De,A.K.,“EnvironmentalChemistry”,NewAgeInternational.

Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


9
BT-301: Thermodynamics and Kinetics L-T-P = 3-0- 0
Atleast30Hrs/Sem

Module I: 8L
Basic Concepts of Thermodynamics: The Ideal Gas, Review of first and second laws of thermodynamics, PVT
behaviourofPureSubstances,VirialEquationofState,ApplicationoftheVirialEquations,CubicEquationsofState.
The Vapour Compression Cycle, the Choice of Refrigerant, Absorption, Refrigeration and liquefaction: Low
temperaturecycle:LindeandClaude.
Module II: 6L
ThermodynamicsanditsApplications:TheChemicalPotentialandPhaseEquilibriaFugacityandFugacityCoefficient:
for pure species and solution; The Nature of Equilibrium, the Phase Rule, Duhem’s Theorem, Simple model’s for
Vapour/LiquidEquilibrium,Roult’sLaw,Henry’slaw,ModifiedRaoult’sLaw.

Module III: 8L
Kinetics: Rate of chemical reaction; Effect of Temperature on Rate Constant, Arrehnius equation, Collision Theory,
TransitionStateTheory,OrderandMolecularityofaChemicalreaction,ElementaryReactions,First,SecondandThird
order reactions, Non Elementary Reactions, Pseudo first order reaction, Determination of rate constant and order of
reaction,Halflifemethod,Fractionalorderreactions

Module IV: 8L
Applications of Kinetics: Interpretation of batch reactor data for simple and complex reactions. Kinetics of Enzyme
catalyzed reactions for free and immobilized enzymes.– derivation of Michaelis Menten equation, Briggs Haldane
relationship, the determination and significance of kinetic constants, Lineweaver burk and Eadie Hofstee plot,
principlesofenzymeinhibition–Competitive,noncompetitiveanduncompetitive.

Textbook :
1.Smith&Vanness,ThermodynamicsforChemicalEngineers,MGH
Reference books:
1.Richardson,J.F.,Peacock,D.G.Coulson&Richardson’sChemicalEngineering Volume3
ed.,FirstIndianed.AsianBooksPvt.Ltd.1998
2.Levenspiel.O.,ChemicalReactionEngineering,WileyEasternLtd.
3.Bailey&Olis,BiochemicalEngg.Fudamentals,MGH,1990
4.PhysicalChemistry:Castellan,NarosaPublishing.
5.PhysicalChemistry,;Moore,PHIBT
BT-302: Biochemistry L-T-P = 3-1-0
Atleast45Hrs/Sem

Module I: 10L
Enzyme Chemistry:
Enzymes,Co enzymesandtheirclassification:Conceptofactivesite,Principlesandmechanismofenzymecatalysis,
enzyme substrate reaction, Classification nomenclature properties cofactors units turnover of enzymes, free energy
and Michaelis Menten equation, activators, inhibitors, inhibition reactions and their kinetics, allosteric and feed back
inhibition competitive, uncompetitive, non competitive inhibition, Hill and Scatchard plot, regulation of enzyme
activity, flexibility and conformational mobility of enzymes, immobilized enzymes, multisubstrate reaction (kinetics,
ping pong and ordered bi bi reactions), multi enzyme systems, isozymes; Regulation of enzyme activity, Allosteric
protein,Isozymes,Solidphasepeptidesynthesis,proteinfolding.


Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


10
Module II: 10L
Carbohydrate Metabolism & Bioenergetics:
GenerationandutilizationofATP.Metabolicpathwaysandtheirregulation:glycolysis,TCAcycle,pentosephosphate
pathway, electron transport chain, oxidative phosphorylation, coupled reaction, group transfer, biological energy
transducers; gluconeogenesis, glycogen (glycogenolysis and glycogenesis) and fatty acid metabolism. Metabolism of
Nitrogencontainingcompounds:nitrogenfixation,aminoacidsandnucleotides.Photosynthesis:theCalvincycle.

Module III: 10L
Metabolism of Amino acid, Protein, Lipids, Nucleic Acids and Vitamins:
CatabolismandAnabolism,Catabolism ofaminoacids, generalmetabolismofaminoacids.Glucogenicaminoacids,
ketogenicaminoacids,ureacycle,proteindegradationandturnover.Betaoxidationandomegaoxidationoffattyacids
– saturated and unsaturated fatty acids – even and odd numbered, catabolism of phospholipids, biosynthesis of fatty
acids and phospholipids. Biosynthesis of cholesterol, neucleotide metabolism purine and pyrimidine degradation;
MetabolismofVitamins.

Module IV: 10L
Cell Communication and Cell Signaling 
Biological membranes; Membrane transport and pumps; Structure of model membrane, lipid bilayer and membrane
protein diffusion, osmosis, ion channels, active transport, ion pumps, mechanism of sorting and regulation of
intracellulartransport,electricalpropertiesof membranes; Cellsignalingandsignaltransduction;Hormonesandtheir
receptors, cell surface receptor, signaling through G protein coupled receptors, signal transduction pathways, second
messengers, regulation of signaling pathways, general principles of cell communication, cell adhesion and roles of
differentadhesionmolecules,gapjunctions,extracellularmatrix,integrins,neurotransmissionanditsregulation.

Textbook:
1.Lehninger,Nelson&Cox,PrinciplesofBiochemistry,CBSPublishers,1993

Reference books:
1.BruceAlberts,MolecularBiologyoftheCell,4thed,GarlandSciencePublishers,2002
2.LubertStryer,Biochemistry,Freeman&Co,NY
3.Voet&Voet,FundamentalsofBiochemistry,JohnWilley&Sons
4.Harper’sIllustratedBiochemistry R.K.Murrayetal.(McGrawHill)
5.OutlineofBiochemistry Conn&Stump(JohnWilley&Sons)
6.ProteinScience A.M.Lesk(OxfordUniv.Press)
7.DNAStructure&Function R.R.Sinden(AcademicPress)
8.TheEnzyme–Dixan&Webb
BT-303: Microbiology L-T-P = 3-1-0
Atleast45Hrs/Sem
Module I: 10L
Introduction to Microbiology:
Basic microbiology  Landmark achievements in 20th century: Refutation of abiogenesis: Major contribution of
scientists.Cellstructureandsubcellularorganellesofbacteria–Slimelayer,capsule,cellwall,flagella,pili,fimbriae,
nucleoid, plasmid and episome (F, R, Ti as example) ribosome, cytoplasmic inclusions (inorganic and organic),
endospores. Archibacteria (importance  structure  reproduction), Moulds (importance  structure  reproduction), yeast
(structure  reproduction), algae (importance  structure reproduction) Virology General classification of virus,
(structure, nucleic acid, cultivation of bacteriophage, coliphage), animal viruses plant viruses, TMV). Importance of
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


11
viruses, life cycle of viruses. Taxonomy  The five kingdom classification, criteria for classification, bacterial
phylogeny,numericalapproach,newapproaches–taxonomicimplicationsofDNAbasecomposition

Module II: 10L
Basic Principles of Microbiology:
Microscopic examination: Principles and applications of dark field, bright field and phase contrast microscopy,
fluorescentmicroscopy,invertedmicroscopy,stereomicroscopyandelectronmicroscopy(TEMandSEM).Resolving
power,numericalapertureandchromaticaberration ofamicroscope.Cultivationofbacteria:Typesofgrowthmedia
(natural, synthetic, complex, enriched, selective  definition with example), pure culture methods (streak plate, spread
plate, pour plate, stab culture, slant culture). Anaerobic (thioglycolate, anaerobic chamber, Robertson’s media,
microaerophilic), liquid shake culture of aerobic bacteria. Control of microbes  Sterilization, disinfection, antiseptic,
tyndallisation, pasteurization: Physical  dry heat, moist heat, UV light, ionizing radiation, filtration, HEPA filter,
Chemicalcompounds,anionicandcationicdetergents.

Module III: 10L
Microbial Growth and Metabolism
Growth of bacteria  Definition, growth phases, kinetics of growth, direct and indirect measurement of growth, The
mathematical natureand expression of growth. growth principles of nutrition,influence ofenvironmental factors pH,
temperature, oxygen, Heavy metals and Other compounds Bacterial metabolism  Aerobic and anaerobic respiration
(definition, examples), fermentation (alcoholic, mixed acid, acetic acid, lactic acid), glycolysis, TCA cycle, Entner
Duodruffs pathway, pentose phosphate pathway, electron transport chain, (components, sites of synthesis), bacterial
photosynthesis (green and purple bacteria), biochemical nitrogen fixation– non symbiotic, symbiotic (definition and
examples),basicconceptofnif–genes.Modgenes,nitrogenasecomplex,legheamoglobin

Module IV: 10L
Microbiology of Air, Water and Soil:
Air microbiology  Microorganisms in the air, sampling techniques, air borne pathogens. Microbiology of water-
Microbiology of fresh water and wastewater (sewage), Important water borne diseases– cholera, typhoid, (name of
pathogen, preventivemeasures).Outlines of method for detection of microorganisms in drinking water (presumptive,
confirmatoryandcompletedtests).Distinctionbetweenfecalandnon fecalcoliforms,IMVICtests.Soilmicrobiology 
Soilmicroorganisms,biogeochemicalcycles(carbon,nitrogen,sulfurcycle),differentkindsofassociationbetweensoil
microflora,betweenmicroandmacroorganisms.
Textbook:
1. R.CDubeyandD.KMaheshwari ATextBookofMicrobiology,3
rd
ed,S.ChandandCompany.
2. C.BPowarandH.FDaginawala GeneralMicrobiology(VolI&II)3
rd
ed,HimalayaPublishingHouse.
Reference books:
1. StanierR.–GeneralMicrobiology,5thed,MacmilanPressltd.
2. M.Pelczar,E.Chan,N.Kreig,Microbiology,5thed,MGH
3. Salle.A.J FundamentalPrinciplesofBacteriology,TataMcgrawHill
4. HansG.Schlegel,GeneralMicrobiology,7thed,CambridgeLowPriceEdns
5. A.H.Rose,ChemicalMicrobiology,3rded,ButterworthWorldStudentReprints


Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


12
PRACTICAL

CH-392: Chemistry -2 Lab L-T-P = 0-0-3
1. AminoAcidAnalysis:pHmeasurementsandBufferPreparation
2. AminoAcidAnalysis:IsoelectricPointDetermination
3. EstimationofproteinsbyLowry’smethod/Biuretmethod
4. EstimationofproteinsbyBradfordAssay
5. DeterminationofN,P,K,organicCfromsoilsamples
6. Lipid/sugar:TLC/PaperChromatography
CH-391: Basic Environmental Engineering and Elementary Biology

Lab


L-T-P = 0-0-3
1. PhysicalexaminationofSewage/Water
a. TotalSolid
b. Totaldissolvesolid
c. Totalsuspendedsolid
d. pH,colorandodor
2. ChemicalestimationofSewage/Waterandsoil
a. DeterminationofChlorides
b. EstimationofChemicaloxygenDemand
3. MicrobialexaminationofSewage/Water
a. Confirmationofcoliforms
b. Biologicaloxygendemand
4.Determinationofsoilmicrobialbiomasscarbon.
5.Examinationofdifferentstagesofcelldivisionusingpreparedslide(MitosisandMeiosis)
6. Examination of different bacteria, algae, fungi, plants and animals by microscopic or morphological 
Examination

BT-392: Biochemistry Lab L-T-P = 0-0- 3

1. Spectrophotometry:VerificationofLambert Beer’slaw
2. Estimationofsugars:Enzymaticmethod
3. Separationofaminoacids/sugars/steroids/vitamins/alkaloids/antibiotics Thinlayerchromatography/Paper
chromatography.
4. Determinationofspecificactivityofanenzyme(αandßamylase/SDH/alkalinephosphatase)
5. EnzymeKinetics (DeterminationofKmandVmax)ofaenzyme
6. DeterminationoftemperatureandpHoptimaofanenzyme
7. EnzymeInhibition–determiningthenatureofinhibition
8. DeterminationofSGOTandSGPT
9. ChemicalestimationofDNA/RNA(EstimationofDNAbydiphenylamine&EstimationofRNAbyorcinol
reagent.)
:
BT-393: Microbiology Lab L-T-P = 0-0-3
1. Sterilizationtechniques
2. Mediapreparation
3. MicroscopyandMicrometry
4. Isolation,enumerationandpurificationofmicrobesfromagivensample
5. StainingTechniques(Simple,Gramstaining,sporestaining)
6. BiochemicalCharacterizationofBacteria
Oxidation/FermentationTest
Catalase,OxidaseandUreaseTests
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


13
IMViCtest
HydrogenSulfideTestandNitrateReductionTest.
CaseinandStarchHydrolysis
7. AntibioticAssay AntimicrobialSensitivityTest(DiscDiffusionMethod)
8. GrowthKinetics(BacterialGrowthCurve)


Semester IV

M(CS)-401:
Numerical Methods
L-T-P = 2-0-0

Approximationinnumericalcomputation:Truncationandroundingerrors,Fixedandfloating pointarithmetic,
Propagationoferrors.(4)
Interpolation:Newtonforward&backwardinterpolation,Lagrange’sandNewton’sdivideddifferenceInterpolation.
(5)
Numericalintegration:Trapezoidalrule,Simpson’s1/3rule,Weddle’srule.(3)

Numericalsolutionofasystemoflinearequations:
Gausseliminationmethod,Matrixinversion,LUFactorizationmethod,Gauss JacobiandGauss Seideliterative
methods.
(6)
NumericalsolutionofAlgebraicequation:
Bisectionmethod,Secantmethod,Regula Falsimethod,Newton Raphsonmethod.
(4)

Numericalsolutionofordinarydifferentialequation:Taylor’sseriesmethod,Euler’smethod,Runge Kuttamethods,
Predictor
CorrectormethodsandFiniteDifferencemethod.(6)
TextBooks: 
1. C.Xavier:CLanguageandNumericalMethods.
2. Dutta&Jana:IntroductoryNumericalAnalysis.
3. J.B.Scarborough:NumericalMathematicalAnalysis.
4. Jain,Iyengar,&Jain:NumericalMethods(ProblemsandSolution).
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


14
References:
1. Balagurusamy:NumericalMethods,Scitech.
2. Baburam:NumericalMethods,PearsonEducation.
3. N.Dutta:ComputerProgramming&NumericalAnalysis,UniversitiesPress.
4. SoumenGuha&RajeshSrivastava:NumericalMethods,OUP.
5. SrimantaPal:NumericalMethods,OUP.


Industrial Stoichiometry
BS-412:!!
L-T-P = 3-1-0
Atleast45Hrs/Sem
Module I: 10L
Small units and dimensions:
Basic physical Laws & concepts of vapour pressure. Buckingham Pi theorem. Dimensionless groups, Conversion of
equations,Solutionofsimultaneousequations,useoflog logandsemi loggraphpaper,triangulardiagram,Graphical
differentiation and graphical integration, Treatment and Interpretation of data, Error analysis in connection with
computation.
Module II: 10L
Material balance:
Introductory Concepts, Simplification of the general mass balance equation for steady and unsteady state processes,
Procedureformaterialbalancecalculations,MaterialBalancewithrecycle,bypassandpurgestreams.Materialbalance
withoutchemicalreactions,humidificationsuchascontinuousfiltration,batchmixing,crystallizer,distillationcolumn
anddrying.Materialbalancewithchemicalreaction:Limitingreactions,excessreactions,yieldandpercentconversion.
Stoichiometryofgrowthandproductformation:growthstoichiometryandelementalbalances.

Module III: 10L
Energy Balance:
General energy balance equation for steady and unsteady state processes: Without Chemical Reaction, concept of
humidificationand psychometric chart. With Chemical Reaction,Enthalpy calculation procedures,Specialcases e.g.,
spraydryer,DistillationColumn,Enthalpychangeduetoreaction:Heatofcombustion,Heatofreactionforprocesses
withbiomassproduction,Energy balanceequationforcellculture,forfermentationprocesses.

Module IV: 10L
Combined Material and Energy Balances
Simultaneousmaterialandenergybalances,selectedindustrialprocesscalculationsforchemicalandbioprocesses.

Textbook:
1.HougenandWatson,ChemicalProcessPrinciples(Partone):2nded,JohnWiley.
Referencebooks:
1.BasicPrinciplesandCalculationsinChemicalEngineering:Himmelblau,6thEd.
PrenticeHall,
2.Bhatt&Vora,Stoichiometry,4thEd.,TM
3.Bioprocessengineering:2
nd
edition,MichaelL.Shuler,FilkertKargi
4.Bhatt&Thakur:Stoichiometry;1
st
ed TataMcGr
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


15

Transfer Operation-I
ES-413: !!
L-T-P = 3-0-0
Atleast30Hrs/Sem
Module I: 8L
BasicconceptsofFluidMechanics:
Dimensional Analysis: Buckingham Pi theorem, Dimensionless groups, Conversion of equations. Classification of
fluids, Newtonian & non Newtonian fluids, basic equation of fluid flow, Continuity equation, Momentum balance
equation, Bernoulli equation, Incompressible laminar flow in pipes, Hagen Poiseullie equation, Friction factor, Flow
throughpacked bed,Fundamentalsoffluidization.
Module II: 6L
Flowmeasurementsandmachineries:
Orifice and Venturi meters, Pitot Tube, Weirs, Rotameters and other types of meters, Transportation of fluids, Pipe
Fittings and valves, Pumps – classification, centrifugal and positive displacement type  peristaltic. Blowers and
Compressors(oil free).
Module III: 6L
Heattransfer
Classificationofheatflowprocesses,basiclaw ofconduction.Onedimensionalsteady stateheatconductionthrough
plan wall composite wall radial systems. Concepts of convection. Heat flow in fluids by conduction and convection.
Countercurrentandparallel flow. Enthalpy balance in heat exchangeequipment. Individual heat transfer coefficients,
overallcoefficient,Heatingandcoolingoffluids,Heattransferequipment.Unsteadystateheattransfer,Radiation.

Module IV: 10L
MechanicalOperations&TransportPhenomena:
Particulate solid: particle size shape, mixed particle size and size analysis. Screen analysis: Types of screens, ideal
screen and real screen, differential and cummulative analysis, screen capacity, industrial screen. Handling of solids:
Conveyors and storage of solids.  Principles of comminution, Types of comminuting equipment, Energy and power
requirement, Crushers, Grinders, Mixing and Agitations, Power consumption in mixing, Mechanical separation,
Screening, Types of screen, Filtration, Principle, Constant pressure and constant rate filtration, Settling classifiers,
Floatation,CentrifugalSeparations.Similarityofmass,momentum,andenergytransfer,Nevier Stoke’sequation,flow
analysisusingN Sequationforflowdownininclinedplane.

Textbook:
1.UnitOperationsofChemicalEngineering:McCabe,Smith&Harriot,TMH,5
th
edition

Reference books:
1.Geankopolis,TransportProcesses&Unitoperations:3rdedition,PHI.
2.Coulson&Richardson,ChemicalEngineering,Vol I&II:,ButterworthHeinemann
3.D.Q.Kern,HeatTransfer,MGH
3.Badger,W.L.,Banchero,J.T.,IntroductiontoChemicalEngineering,MGH
4.Foust,A.S.,Wenzel,L.A,et.al.PrinciplesofUnitOperations,2ndedition,JWS
5.Perry,Chilton&Green,ChemicalEngineers’Handbook,MGH
6. R.ByronBird, WarrenE.StewartandEdwinN.Lightfoot:Transportphenomenon,JohnWiley&SonsInc.Asian
studentsEdition.6
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


16
BT-401: Molecular Biology L-T-P = 3-1-0
Atleast45Hrs/Sem
Module I
Introduction to molecular biology: 10L
DNA and RNA Structure of DNA:Nucleoside, Nucleotide, Base pairing, Base stacking, Double Helix,features of
WatsonandCrickmodel,majorandminorgroove.FormsofDNA A,B,Z.StructureandfunctionofmRNA,rRNA,
tRNA.SecondarystructuresinRNA
Module II
DNA Replication and repair: 10L
TypesandfunctionsofDNApolymerasesinProkaryoteandEukaryoteReplicationinprokaryoteandEukaryote,Proof
readingactivity, 5’3’exonucleaseactivity,topoisomeraseactivity,TelomericDNA.DNARepair Nucleotideexcision
repair, base excision repair, mismatch repair, photo reactivation, recombination repair and SOS repair. Recent
advancementsinDNArepair.
Module III
Transcription and translational machinery: 10L
Structure and function of the promoters, RNA polymerases in prokaryote and eukaryote: types and functions.
Transcription of mRNA, rRNA,and tRNA genes in prokaryotes and eukaryotes. Transcription termination (Rho
dependentandindependent),PosttranscriptionalprocessingofmRNA–5’capping,splicing(includingdifferenttypes),
polyadenylation. Genetic code and Wobble hypothesis Translation in prokaryote and eukaryote Post translational
modifications:typesandsubcellularlocationsofmodifications.Inteinandexein,Inteinsplicing.

Module IV
Gene regulation: 10L
Geneexpression–conceptofoperon(lac,trpandara),Phageregulatorystrategy(Transcriptionalcontrolinlyticand
lysogeniccycletakinglamdaasmodel),geneexpressionineukaryotes:typesandcontrolsystems.Recentdevelopment
ingeneexpressionmechanisms.
Textbook:
1.OldandPrimrose,PrinciplesofGeneManipulation,3rdEd,BlackwellScientific
Publishers.2
2.Watsonetal,MolecularBiologyoftheGene3rded,Pearson
Reference books:
1.Watson,J.D.,Gilman,M.,Witkowski,J.,Zoller,M.  RecombinantDNA,Scientific AmericanBooks,NewYork,
1992.GlickandPasternwck,MolecularBiotechnology.
2.BenjaminLewin,GenesVIII,Internationaledition,Pearson.

BT-402: Industrial Microbiology and Enzyme Technology L-T-P = 3-1-0
Atleast45Hrs/Sem
.
Module I: 10L
Commercial Strain Development & Microbial Processes: Cellular control regulating production of microbi al
metabolites–PrimaryandSecondarymetabolite–Inducedmutationtechnique–Analogueresistantmutant–Catabolic
derepressedmutants–Geneticallyengineeredstrain–Protoplastfusiontechnique.
Basic idea on fermentation process, submerged, stationary, solid and semi solid – with their merits and demerits,
Outlines of all microbial processes like productions of organic acids, solvent, antibiotic, polysaccharide, enzymes,
vitamins, lipids, pigments, aroma, Classical process may be discussed in details: Wine and spirits; Acetone butanol;
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


17
Penicillin/Tetracycline/Streptomycin fermentation; Alkaline protease/lipase/amylase; Citric acid;  Dextran, xanthan
gum
Module II: 10L
Introduction to Fermentation Technology; Microbial Culture systems; Media for Industria fermentations; Media
Optimization; Sterilization of Industrial Media; The development of Inocula for Industrial fermentations; Starter
Cultures;DownstreamProcessingandfermentationeconomics.

Module III: 10L
Application of Enzyme and Enzyme Technology: Extraction and Purification of Crude Enzyme extracts from plant,
animal and microbial sources some case studies; Brief Enzyme Overview; Classification & Nomenclature;Storage &
Handling;Units of Activity;General Characteristics; Environmental Effects on Enzyme Activity;  Glycosi dic
Hydrolases;  Cellulases. Pectic Enzymes, Proteolytic Enzymes, Esterases, Lipase, Oxidoreductases, Lipoxygeanase.
Stabilityofenzyme,strainselection,(thermophilic,halophilic,alkalophilicproducerstrain),Cloningstableenzymein
mesophile,Proteinengineeringtoimproveenzymestability,Enzymeapplications–(Industrial,medicalandanalytical),
Reaction environment rebuilding, Enzyme reaction in non aqueous medium, Synthesis with hydrolase enzymes,
Chemical modification of enzyme to improve physico chemical properties, Immobilization of enzymes, Various
techniques

Module IV: 10L
Enzyme Immobilisation Technology: Physical and Chemical techniques for enzyme Immobilization – adsorption,
matrix entrapment, encapsulation, cross linking, covalent binding – examples. Advantages and disadvantages of
different Immobilization techniques. Overview of applications of immobilized enzyme systems, Applications of
enzymes in analysis; Design of enzyme electrodes and their applications as biosensors in industry, health care and
environment.DesignofImmobilizedEnzymeReactors Packed bed,Fluidized bedMembranereactors;Bioconversion
calculationsinfree enzymeCSTRsandimmobilizedenzymereactors.

Text books:
1. L.E.Cassida.Jr,IndustrialMicrobiology,NewAgeInternationalPublisher
2. W.Crueger,AnneliseCrueger,Biotechnology:ATextbookofIndustrialMicrobiology,SinauerAssoc.Inc
3. FundamentalsofenzymologybyNicolasC.priceandLewisstevens.OxfordUniversityPress
4. EnzymesbyTrevorpalmer,EastwestPress3.EnzymeTechnologybyMessing

Reference books:
1. Prescott’sandDunn’s,A.IndustrialMicrobiology,4thedition.CBSPublishers,NewDehli,India,1987.
2. Atkinson.BandMarituna.F,BiochemicalEngineeringandBiotechnologyHandbok,TheNaturePress,
MacmillanPubl.Ltd.4
3. Enzymes:DixonandWebb.(IRLPress)EnzymetechnologybyChaplinandBucke.CambridgeUniverity
Press
4. Biochemicalengineeringfundamentals,secondedition.JamesEBailey,DavidF.,Ollis,McGrawHillIntl.
Edition


Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


18
PRACTICAL
Communication Skill and Report Writing
HU-481:
L-T-P = 0-0-3
Cr-2
Guidelines for Course Execution:
Objectives of this Course: This course has been designed:
1. To inculcate a sense of confidence in the students.
2. To help them become good communicators both socially and professionally.
3. To assist them to enhance their power of Technical Communication.

DetailedCourseOutlines:
A. Technical Report Writing:2L+6P

1. ReportTypes(Organizational/Commercial/Business/Project)
2. ReportFormat&OrganizationofWritingMaterials
3. ReportWriting(PracticeSessions&Workshops)
B.Language Laboratory Practice

I. Introductory Lecture to help the students get a clear idea of Technical Communication & the need of Language
Laboratory
Practice Sessions 2L
2. Conversation Practice Sessions: (To be done as real life interactions)
2L+4P
a) Training the students by using Language Lab Device/Recommended Texts/cassettes /cd’s to get their Listening
Skill & Speaking Skill honed
b) Introducing Role Play & honing over all Communicative Competence
3. Group Discussion Sessions: 2L+6P
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


19
a) Teaching Strategies of Group Discussion
b) Introducing Different Models & Topics of Group Discussion
c) Exploring Live /Recorded GD Sessions for mending students’ attitude/approach & for taking remedial measure
Interview Sessions; 2L+6P
a) Training students to face Job Interviews confidently and successfully
b) Arranging Mock Interviews and Practice Sessions for integrating Listening Skill with Speaking Skill in a
formal situation for effective communication

4. Presentation: 2L+6P
a) Teaching Presentation as a skill
b) Strategies and Standard Practices of Individual /Group Presentation
c) Media & Means of Presentation: OHP/POWER POINT/ Other Audio-Visual Aids

5. Competitive Examination: 2L+2P
a) Making the students aware of Provincial /National/International Competitive Examinations
b) Strategies/Tactics for success in Competitive Examinations
c) SWOT Analysis and its Application in fixing Target

Books – Recommended:
Nira Konar: English Language Laboratory: A Comprehensive Manual
PHI Learning, 2011
D. Sudharani: Advanced Manual for Communication Laboratories &
Technical Report Writing
Pearson Education (W.B. edition), 2011
References:
Adrian Duff et. al. (ed.): Cambridge Skills for Fluency
A) Speaking (Levels 1-4 Audio Cassettes/Handbooks)
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


20
B) Listening (Levels 1-4 Audio Cassettes/Handbooks)
Cambridge University Press 1998
Mark Hancock: English Pronunciation in Use
4 Audio Cassettes/CD’S OUP 2004

Numerical Methods Lab
M(CS)-491 :
L-T-P = 0-0-2


1. AssignmentsonNewtonforward&backward,Lagrange’sinterpolation.
2. AssignmentsonnumericalintegrationusingTrapezoidalrule,Simpson’s1/3rule,Weddle’srule.
3. AssignmentsonnumericalsolutionofasystemoflinearequationsusingGausselimination,Matrixinversion,
Gauss Jacobi,andGauss Seideliterations.
4. AssignmentsonnumericalsolutionofAlgebraicEquationbyBisection,Secant,Regular falsiandNewton
Raphsonmethods.
5. Assignmentsonordinarydifferentialequation:Taylorseries,Euler’s,Runga KuttaandFinitedifference
methods.
6. IntroductiontoSoftwarePackages:Matlab/Scilab/Labview/Mathematica.

Transfer Operation I Lab
ES-483:!!
L-T-P = 0-0-3 
1. ExperimentsonReynold’sApparatus Determinationofflowregimeandconstructionoffrictionfactoragainst
NRe.
2. Experiments on flow measuring device—in closed conduit using (a) Venturimeter, (b) Orifice meter (c)
Rotameter.
3. Determination of Pressure drop for flow through packed bed & verification of Ergun Equation, Kozeny
Karmanequation,Blake PlummerEquation.
4. TostudytheworkingcharacteristicsofaJawCrusher,calculatetheenergyconsumptionasafunctionofsize
reductionandcompareitwiththeactualenergyrequirements.
5. To study the working characteristics of a Ball Mill, calculate the energy consumption as a function of size
reductionanddeterminethecriticalspeed.
Syllabus for B.Tech(Biotechnology) Second Year
Revised & Proposed Syllabus of B.Tech in EE (To be followed from the academic session, July 2011, i.e. for the students who were
admitted in Academic Session 2010-2011)


21
6. To Determine the Overall heat transfer coefficient of a concentric pipe heat exchanger based on the inside
diameterofthetube.
7. Tostudythecharacteristicsoffilm wise/drop wisecondensation.

BT-491: Molecular Biology Lab L-T-P = 0-0-3

1. IsolationofGenomicDNAfromblood,plantcellandfrombacteriaofdifferentsourceslikesoil,wateretc.
2. SpectroscopicanalysisofDNA/RNA
3. PreparationofAgaroseGel
4. IsolationofPlasmids
5. SeparationofproteinsbyPolyacrylamideGelElectrophoresis(SDS/PAGE).
6. IsolationofRNA
7. FormaldehydegelelectrophoresisofRNA
8. Inducedmutationby:(a)Chemical(b)Ultravioletlight
9. PhageTitration

BT-492: Fermentation Technology Lab L-T-P = 0-0-3


1. BatchFermentationandRecoveryandAssayofAntibiotics(Penicillin/Streptomycin
orany
oftheinstitutionschoice
)
2. ProductionofAlcohol(FermentationandRecovery)
(i) UsingMolasses/SugarcaneJuice(Batch)
(ii) UsingImmobilizedMo’s(BatchandContinuous)
3. BatchFermentationofOrganicAcid
4. BatchandImmobilizedFermentationofBacterial/FungalEnzymes(Amylases/Proteases
oranyenzymeoftheinstitutionschoice
)(Fermentation,RecoveryandAssay).
5. SolidStateFermentation(Moulds/Fungus).