Ene 487 environmental biotechnology and bioremediation


12 Φεβ 2013 (πριν από 5 χρόνια και 5 μήνες)

174 εμφανίσεις

ENE 487


Course Description

Environmental biotechno]ogy utilizes microorganisms to improve environmental quality.
These improvements include treatment of contaminated waters and wastewaters, clean up of
industrial waste streams, and remediation of soils contaminated with hazardous and toxic
chemicals. Environmental biotechnology is essential to society and truly important as a technical

Environmental biotechnology is historic and eminently modern. Microbiological
treatment technologies developed at the beginning of the twentieth century, such as trickling
filtration, activated sludge and anaerobic digestions remain the mainstays today. In recent years,
new technologies are constantly introduced that address very contemporary problems such as
detoxification of hazardous chemicals, environmental biomonitoring, and microbial genetic
engineering for bioremediation of air, water, and soil.


Environmental Biotechnology : Principles and
Rittmann, B.E., and McCarty, P.L.,
McGraw Hill, 2001.

Class Notes

“Applied Environmental Microbiology and Case Studies,” prepared by M. Pirbazari, 2002.


Prescott, L. M., Harley, 3. P., and Klein, D. A., Microbiology, Second Edition, Wm. C. Brown
Publishers, Dubuque, Iowa, 1993.


CE 210L and BISC 320L


Grading Criteria

Midterm Exam 20%
Final Exam 30%
Howework 15%
Term Paper and Oral Presentation 25%
Class Participation

Field Trips

Two field trips to bioremediation sites will be arranged.

Laboratory Demonstration:

Applications of light microscopy, fluorescence microscopy, and electron microscopy to
environmental samples will be demonstrated in the laboratory.

ENE 487


Course Objectives

The proposed course is designed to teach students the scientific and engineering
principles of microbiological treatment technologies to clean up contaminated environments and
to generate valuable resources for the human society. The course Will include the following
components: (I) fundamentals of environmental microbiology, (2) bioremediation of organic
contaminants and toxic metals, (3) biodegradation of problem environmental contaminants, and
(4) engineering strategies for bioremediation.

Course Syllabus

(Week 1)
Fundamental Aspects of Environmental Microbiology
• Structure and Functions of Procaryotic Cells
• Structure and Functions of Eucaryotic Cells
• Taxonomy of Microorganisms: Bacteria, Algae, Fungi and Protozoa
• Study of Microbial Structure
-Light Microscopy
-Dark-field and Phase-contrast Microscopy
-Electron Microscopy
• Environmental Significance of Bacteria, Fungi, and Algae

(Week 2)
Microbial Metabolism, Growth and Biokinetics
• Microbial Nutrition and Metabolism
• Microbial Growth and Energy
• Enzymes and Their structures
• Effect of Environment on Enzyme activity
• Microbial Growth and Substrate Utilization Kinetics
• Biokinetic Models
• Batch and Continuous Chemostat Studies
• Determination of Biokinetic Parameters
• Examples of Growth Kinetics in Engineered Systems(air, water, and soil)


(Weeks 3 and 4)
Microbial Genetics
• General Principles of Recombination and Plasmids
• DNA Transformation
• Recombinant DNA Technology
• Polymerase Chain Reactions
• Isolating and Cloning Fragments
• Concept of Gene Probes
• Fundamentals of Cloning
• Insertion and Expression of Foreign Genes
• Recombinant DNA Techniques in Biotechnology
• Applications in Environmental Engineering

(Week 5)
Microbiology Reactions
• Suspended Growth Reactors
• Biofilm Reactors
• Batch Reactors
• Completely Stirred Tank Reactors
• Plug Flow Reactors
• Reactors in Series
• Engineering Design of Reactors

(Week 6)
Bioflim Processes
• Trickling Filters and Biological Towers
• Rotating Biological Contactors
• Granular Media Filters
• Fluidized-bed Reactors
• Hybrid Biofllm Processes

(Weeks 7 and 8)
Bioremediation for Soil Environment
• Environment of Soil Microorganisms
• Soil Organic Matter and Characteristics
• Soil Microorganisms Association with Plants
• Pesticides and Microorganisms
• Petroleum Hydrocarbons and Microorganisms
• Industrial solvents and Microorganisms
• Biotechnologies for Ex-Situ Remediation of Soil


• Biotechnologies for in-Situ Remediation of Soil
• Phytoremediation Technology for Soil Decontamination

(Week 9)
Bioremediation for Air Environment
• Atmospheric Environment for Microorganisms
• Microbial Degradation of Contaminants in Gas Phase
• Biological Filtration Processes for Decontamination of Air Stream
- Biofiltration
- Biotrickling Filtration
- Bioscrubbers

(Weeks 10 and 11)
Bioremediation for Water Environment
• Biochemical, Molecular, and Ecological Foundations of Bioremediation
• Contaminants in Groundwater

Ex-situ Decontamination of Groundwater
Characterizing the Site and Contaminant Complexity
-Selecting the Bioremediation Option
• Process Optimization
• In-situ Bioremediation of Groundwater
-Factors Affecting Bioaugmentation
-Delivery Systems for Oxygen, Nutrients, and Innoculation
• Landfill Leachate Biotreatment Technologies
• Industrial Wastewater Biotreatment Technologies
• Biotreatment of Surface Waters

(Week 12)
Biotreatment of Metals
• Microbial Transformation of Metals
• Biological Treatment Technologies for Metals Remediation
• Bioleaching and Biobenificiation
• Bioaccumulation
• Oxidation/Reduction Processes
• Biological Methylation
• Case studies

(Week 13)
Overcoming Limitations of Bioremediation
• Factors Affecting the Bioremediation Processes
• Effects of Co-substrates on Microorganisms
• Global Application of Bioremediation Technologies
• Successful and Unsuccessful Case Studies

(Week 14)
Emerging Environmental Biotechnologies
• Phytoremediation
• Sequestering Carbon Dioxide
• Biomonitoring
• Application of Microbial Enzymes
• Biomembrane Reactors

(Week 15)
Case Studies in Environmental Biotechnology
• Environmental Biotechnology Research Activities in European Union
• Environmental Biotechnology Research Activities in Japan
• Environmental Biotechnology Research Activities in US
• Environmental Biotechnology Research Activities in Other Countries

(Attention: term papers are due during the week of final exams)

Note: Any student requesting academic accommodations based on a disability is required to
register with Disability Services and Programs (DSP) each semester. A letter of verification for
approved accommodations can be obtained from DSP. Please be sure the letter is delivered to me
(or to TA) as early in semester as possible. DSP is located in STU 301 and is open 8:30 a.m. -
5:O0.p.m., Monday through Friday. The phone number for DSP is (213) 740-0776.