Review: Viruses and Biotechnology

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23 Οκτ 2013 (πριν από 3 χρόνια και 10 μήνες)

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Name:____________________________________


Date of Test:___________

Review: Viruses and Biotechnology

Ch. 19 Viruses

1.

What are the components of a virus?

2.

Draw and explain the lytic and lysogenic cycles (you may sketch them together)

3.

How to retroviruses reproduce? Sketch what happens and explain each step using
captions on

your drawing.

4.

What is the difference between an epidemic and a pandemic?

5.

In order for a pandemic to occur what must the virus be able to do?

6.

What is a vaccine and how does it work?

7.

What are viroids? What type of organism do they affect?

8.

What are prions?

a.

What do they affect?

b.

Where do they come from?

c.

How do they work, and list an example

9.

Draw a Venn diagram comparing bacteria and viruses

10.

In what ways can new viruses emerge? (3 ways)

11.

If a bacterium was infected with an engineered phage that contained a T2 pr
otein coat,
but T4 DNA, what would occur within the cell?

Ch. 20 Biotechnology

1.

What is a plasmid and how is it used as a tool in biotechnology?

2.

List the uses of each of the following biotechnology tools:

a.

Restriction enzyme

b.

DNA ligase

c.

DNA polymerase

d.

Reverse

transcriptase

e.

Electrophoresis

f.

Plasmid
/Bacterial Transformation

3.

Why are plants more easily manipulated by genetic engineering than animals?

4.

List several ways DNA technology is used in the medical field?

5.

What is a vector in DNA technology?

6.

What is cDNA

and how is it made?

7.

Which of the following DNA sequences is most likely to be recognized by a restriction
enzyme?

a.

AATTCGA

Name:____________________________________


Date of Test:___________

TTAAGCT

b.

GGCC

CCGG

c.

ACCAT

TGGTA

8.

Why did you choose the one you did in question #7?

9.

What issues arise when incorporating foreign eukaryo
tic DNA into prokaryotic DNA. Be
specific.

10.

Describe the technique of Gel electrophoresis and explain how it works.

11.


List the uses for gel electrophoresis.

12.

Explain how stem cells work. What are two different types? How are they different
from one another
?

13.

Briefly describe the cloning of a mammal. (page 414 in the online text) See figure 20.19

Be sure you know the following terms. Some you may have to look up in the text.

Biotechnology Vocabulary List

Anneal

cDNA

DNA ligase

DNA probes

Gel Electrophoresis

Gene Cloning

Gene Therapy

Genetic Profile

PCR

Pla
smids

Pluripotent

Recombinant DNA

Restriction enzymes (endonucleases)

Restriction Sites

RFLPs

Stem Cells

Sticky ends

Totipotent

Transformation

Transgenic

Vectors