Prof. B.S. Srivastava - Biotechresearch

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1


Professor Brahm S. Srivastava, Ph.D.




Former





*
Scientist in Director Grade & Head, Microbiology Division, Central Drug Research
Institute, Lucknow


*
Professor & Founder Chairman, Centre for Biotechnology, Jawaharlal Nehru University,
New Delhi

*
Emeritus Scientist (C.S.I.R.), Central Drug Research Institute, Lucknow


*
L
ecturer, School of Basic Sciences & Humanities, Udaipur University, Udaipur


*
Visiting Scientist, Centre for Vaccine Development, University of Maryland at
Baltimore, USA


*
Visiting

Scientist, University of Ancona, Italy


*
Visiting Scientist, Pasteur Institute, Lille, France







2

Academic Profile

Prof Srivastava,
winner of
Sir Shanti Swaroop Bhatnagar award
, CSIR,
graduated from
Banaras Hindu University and obtained B.Sc, M.Sc. and Ph.D degrees. He did
postdoctoral research

at the University of Brussels. He was visiting scientist at Brown
University (USA), University of Maryland (USA), Institute Pasteur Lille (Fra
nce) and
University of Ancona (Italy).


He had the honour and satisfaction to start the Masters Programme in Biotechnology
at Jawaharlal University and to be the member of the Task Force of DBT on Human
Resource Development in Biotechnology. He has serv
ed on scientific and academic
bodies of DBT, ICAR, CSIR, DST, ICMR, UGC and several universities.
He

was member of
the INDO
-
US Vaccine Action Programme and of the Indian delegation to USA and China
on Biotechnology.

Academic Awards

& Fellowships


B.H.U.
Prize for standing Ist in merit in M.Sc. Examination.


S.S. Bhatnagar Prize in Medical Sciences, C.S.I.R., 1984.

Dr. Nitya Anand Endowment Lecture, INSA, 1991.

Dr. Y.S. Narayana Rao Oration Award, ICMR, 1993.

Shri Om Prakash Bhasin Award for Science & Tech
nology, 1995.

Vigyan Ratna Samman, UP Government, National Technology Day, 2002.


Fellow, The National Academy of Sciences, India.

Fellow, American Academy of Microbiology.



Sources of Research Funding


World Health Organization

Department of Biotechnolog
y

Indo
-
US Vaccine Action Programme


3

Indo
-
Italian Scientific & Technical Bilateral Cooperation

Indo French Cooperation for Promotion of Advanced Research

Department of Science & Technology

Council of Scientific & Industrial Research


Centre for Biotechnol
ogy, Jawaharlal Nehru University, New Delhi

S
elected
,

the first Professor & Chairman of the Centre and invited to start teaching and
research in Biotechnology and to coordinate with other 5 Centres for Biotechnology in
BHU, Baroda, Pune, Madurai and Jadav
pur.



Significant Research Contribution


1.

Developmental Biology and DNA repair mechanisms in cyanobacteria.



2.

DNA repair in
E. coli

and bacteriophages
.
A

new gene locus,
rer
, was identified which
facilitated repair of UV
-
induced damage by regulating the coordination between
repair of damage and replication
.
The
rer
gene has been incorporated on the
linkage map of
E. coli.


3.

Pathogenesis of cholera.

L
andmark discovery
on
dem
onstrat
ion of

expression and
modulation of cholera toxin biosynthesis

by plasmids

(Nature, 1978)
and
adhesive
protein

of
V. cholerae

for binding to intestinal mucosa and
the concept of the
subunit vaccine in experimental cholera
.


4.

Tuberculosis.

Identif
ication and development of
a reliable reagent for early
diagnosis of TB
,

user friendly non hazardous
genetic
screens for antitubercular
compounds
,

strategies to identify genes which are important for tubercle bacilli to

4

survive in the host

and role of m
ycobacterial lipase in virulence and modulation of
host immune response.


Major Research Publications

1.

Singh, H.N. and Srivastava, B.S. : Studies on morphogenesis. I. Effect of inorganic
nitrogen sources on developmental morphology of

Anabaena

doliolum
.
Can. J.
Microbiol. 14,

1968, 1341.


2.

Srivastava, B.S.: Ultraviolet induced mutations to growth factor requirement and
penicillin resistance in a blue green alga.
Arch. Mikrobiol. 66
, 1969, 234.


3.

Srivastava, B.S. and Kumar, H.D.: Inactivation and killing of
Anabaena doliolum

grown on ultraviolet irradiated culture medium.
Radiation Botany 9
, 1969, 291.


4.

Srivastava, B.S.: Sensitivity and resistance of a blue green alga to streptomycin and
penicillin
. Arch. Mikrobiol. 72
, 1970, 182.


5.

Srivastava, B.S.: A simple method of isolating ultraviolet resistant and sensitive
strains of a blue green alga.
Phycologia (USA) 9
, 1970, 205.


6.

Srivastava, B.S.: Pigment variation in ultraviolet irradiated
Anabaena doliolum
.
Arch.
Mikrobiol. 77,

1971, 274
-
77.


7.

Srivastava, B.S., Kumar, H.D. and Singh, H.N.: The effect of caffeine and light on
killing of
Anabaena doliolum

by ultraviolet radiation.
Arch. Mikrobiol. 78
, 1971,
139.


8.

Srivastava, B.S.: Repair of gamma ray induced damage in bacteriophage lambda.
V
irology 55,

1973, 550.


9.

Srivastava, B.S.: The yield of single
-
strand breaks in the DNA of bacteriophage
lambda irradiated extra and intracellularly with gamma rays in oxic and anoxic
conditions.

Int. J. Radiat. Biol. 26
, 1974, 391.


10.

Lafonteyne, J. and Sriv
astava, B.S. Replication and recombination dependent
maturation of phage lambda.
Zeitschrift fur Physiologische Chemie. 355
, 1974, 54


5

11.

Srivastava, B.S.: Radiation sensitivity of a mutant of
Escherichia coli

K
-
12 associated
with DNA replication: Evidence for

a new repair function.
Molec
.
Gen. Genet. 143
,
1976, 327.


12.

Srivastava, B.S.: Irradiation and DNA breaks.
Nature (Lond.) 259
, 1976, 425.


13.

Ahmad, M., Srivastava, B.S. and Agarwala, S.C.: Purification of bacteriophage
lambda by gel filtration.
Ind. J. Expt.

Biol. 15
, 1977, 387.


14.

Ahmad, M., Srivastava, B.S. and Agarwala, S.C. : Effect of incubation media on the
recovery of
Escherichia coli

K
-
12 heated at 52
o
C .

J. Gen
.
Microbiol. 107
, 1978, 37.


15.

Srivastava, B.S.: The role of inducible gene

rer

of

Escherichia coli

K
-
12 in DNA repair
and mutagenesis.
Mutation. Res
.
52,

1978, 1.


16.

Sinha, V.B. and Srivastava, B.S. : Plasmid induced loss of virulence in
Vibrio
cholerae
.
Nature (Lond.) 276
, 1978, 708.


17.

Ahmad, M. and Srivastava, B.S: Thermal inactivation
and mutagenesis of
bacteriophage lambda.
Mutation Res. 73
, 1980, 403.


18.

Rai, R., Prasad, V and Srivastava, B.S.: Symbiotic nitrogen fixation efficiency of
mutant strains of Rhizobium growing from pH 4.5 to 7.5

J. Agric. Sci. Camb. 98
,
1982, 487.


19.

Sinha
,
V.B
. and Srivastava, B.S.: Suppression of pathogenecity by P and V plasmids
in
Vibrio cholerae
. J. Gen. Microbiol. 104
, 1978, 251.


20.

Bhattacharjee, J.W. and Srivastava, B.S.: Mannose
-
sensitive haemagglutinins in
adherence of
Vibrio cholerae

el tor to intestine
. J. Gen. Microbiol. 107
, 1978, 407.


21.

Bhattacharjee, J.W. and Srivastava, B.S.: Adherence of the wild
-
type and mutant
strains of
Vibrio cholerae

to normal and immune intestinal tissue.
Bull
.
Wld. Hlth.
Org. 57
, 1977, 123


22.

Srivastava, R., Sinha, V.B. and Sr
ivastava, B.S.: Re
-
evaluation of antibacterial and
antitoxin immunities in experimental cholera.
Ind. J. Med. Res. 70
, 1979, 368.


23.

Sinha, V.B. and Srivastava, B.S.: Attenuation of virulence by P and V plasmids in

Vibrio cholerae
: strains suitable for oral
immunization.
Bull. Wld
.
Hlth. Org. 57
,
1979, 643.



6

24.

Srivastava, B.S., Sinha, V.B. and Srivastava, B.S.: Attenuated recombinant strains of
Vibrio cholerae

for oral immunization.
Bull. Wld. Hlth. Org. 57
, 1979, 649.


25.

Srivastava, R., Sinha, V.B. and Srivastav
a, B.S.: Events in the pathogenesis of
cholera: role of bacterial adherence and multiplication.
J. Med. Microbiol. 13
, 1980.
1.


26.

Srivastava, R
.

and Srivastava, B.S.: Isolation of a non adhesive mutant of
Vibrio
cholerae

and chromosomal localization of the gene controlling mannose sensitive
adherence.

J. Gen. Microbiol. 117
, 1980, 275.

27.

Knopf, P.M. and Srivastava, B.S.: Application of cloning techniques to the
development of a synthetic vaccine against schistosomiasis.
Ve
t. Parasitol
.
10,

1982,
255.


28.

Sinha, V.B. and Srivastava, B.S.: Plasmid associated suppression of pathogenicity of
some wild type strains of
Vibrio cholerae

isolated from human cholera patients.
Ind.
J. Med. Res. 77
, 1983 1.


29.

Barker, R.H., Srivastava, B.S.
, Goldberg, M. and Knopf, P.M. : Immunoprecipitation
analysis of radiolabelled antigens synthesized
in vitro

by
S. mansoni

I. Identification
of antigens uniquely recognized by protective antibodies
. J. Immunol. 134
, 1985,
1129.


30.

Khan, A
.A., Srivastava, R.,

Sinha, V.B. and Srivastava, B.S.: Regulation of toxin
biosynthesis by plasmids in

Vibrio cholerae.

J. Gen. Microbiol. 131
, 1985, 2653.


31.

Srivastava,

R., Khan, A.A. and Srivastava, B.S.: Immunological detection of cloned
antigenic genes of
Vibrio cholerae

i
n
Escherichia coli
.
Gene, 40
, 1985, 267.


32.

Srivastava, R. and Srivastava, B.S.: Molecular biology of the mating types in yeast
Saccharomyces cerevisiae
.
Jour. Pl. Sci. Res. 1
, 1985, 48.


33.

Sinha, V.B. and Srivastava, B.S.: Colonization and protection in infan
t rabbits by a
choleragenoid producing strain of
Vibrio cholerae
.
Ind. J. Med. Res. 84
, 1986, 139.


34.

Srivastava, R
., Sinha, V.B. and Srivastava, B.S.: Chromosomal transfer and in vivo
cloning of genes in
Vibrio cholerae

using RP4:: mini
-
Mu.
Gene, 75
, 1989,
253.



7

35.

Srivastava, R., Ali, S.S. and Srivastava, B.S.: Cloning of xylanase gene of
Streptomyces flavogriseus

in

Escherichia coli

and bacteriophage lambda induced
lysis for the release of cloned enzyme.
FEMS Microbiol Lett. 78

(2/3) 1991, 20.


36.

Czeczulin, J.R
., Srivastava, R., Srivastava, B.S. and Kaper, J.B. : Characterization of a
possible colonization factor of
Vibrio cholerae

01.
Proc. Amer. Soc
.
Microbio
l, 1990,
108.


37.

Kaper, J.B. and Srivastava, B.S. : Genetics of cholera toxin.

Ind. J. Med. Res
. 1992,
16
3.


38.

Jacob, A., Sinha, V.B., Sahib, M.K., Srivastava, R., Kaper, J.B. and Srivastava, B.S.:
Identification of a 33 kDa antigen associated with an adhesive and colonizing strain
of
Vibrio cholerae

el tor and its role in protection.
Vaccine,

11
, 199
3,

376.


39.

Sinha,V.B. , Jacob, A., Srivastava R. , Kaper, J.B and Srivastava, B.S.:

Identification of
the flagellar antigens of

Vibrio cholerae

el tor and their role

in protection.
Vaccine,
11
, 1993, 372.


40.

Srivastava, B.S.: Pathogenesis of cholera and vaccine devel
opment.
Proc.

Ind. Nat.
Sci. Acad., 59B,
1993, 459
.


41.

Krishna Kumar, K, Srivastava,R, Sinha, V.B. Mechalski, J., Kaper, J.B. and Srivastava,
B.S. recA mutations reduce adherence and colonization of

classical and el tor
strains of
Vibrio cholerae
.
Microbio
logy 140
, 1994, 1217.


42.

Chandrasekhar, U., Sinha, S., Bhagat, H.K., Sinha, V.B. and Srivastava, B.S.:
Comparative efficacy of biodegradable liposomes and microspheres as carriers for
delivery of
V.cholerae

antigens in the intestine.
Vaccine 12
, 1994, 1384.


43.

Singh, S.N., Srivastava, R., Sinha, V.B. and Srivastava, B.S. : A 53 kDa protein of
Vibrio cholerae

classical strain 0395 involved in intestinal colonization.
Microbial
Pathogenesis 17,

1994, 69.


44.

Kumar, D., Srivastava, B.S., Singh, N.B. and Srivastava, R
.: Identification of a 25 kDa
protein of
Mycobacterium bovis

BCG to distinguish BCG from
Mycobacterium
tuberculosis
.

J. Clin. Microbiol. 34
, 1996, 224.



8

45.

-

galactosidase
reporter system in mycob
acteria and its application in rapid

antimycobacterial drug
screening.
Biochem. Biophys. Res. Commun
.
235,


1997, 602.


46.

Srivastava, R., Kumar, D., and Srivastava, B.S.: Recombinant
Mycobacterium

aurum

expressing

Escherichia coli

-
galactosidase in high
through put

screening of
antituberculosis drugs.
Biochem. Biophys. Res. Commun. 240,

1997, 536.


47.

Bondre, V.P., Srivastava, R., Sinha, V.B., and Srivastava, B.S. : Screening of Tn
pho
A
mutants of
Vibrio cholerae
0139 for identification of antigens involved
in
colonization
. J. Med. Microbiol. 46
, 1997, 1007.


48.

Bondre, V.P., Sinha, V.B. and Srivastava, B.S. : Evaluation of different subcellular
fractions of

Vibrio cholerae

0139 in protection to challenge in experimental cholera.
FEMS Immunol. Med. Miccrobiol. 1
9
, 1998, 323.


49.

Shukla, A., Dubey, M.P., Srivastava, R. and Srivastava, B.S .: Differential expression
of proteins during healing of cutaneous wounds in experimental and normal and
chronic models.
Biochem. Biophys. Res. Commun. 244
, 1998 434.


50.

Kumar, D., Sr
ivastava, B.S. and Srivastava, R.: Genetic rearrangements leading to
disruption of heterologous gene _expression in mycobacteria: An observation with
-
galactosidase in
Mycobacterium smegmatis
.
Vaccine, 16
, 1998, 1212.


51.

Srivastava, R., Deb, D.K., Srivastav
a, K.K. Locht, C. and Srivastava, B.S.: Green
fluorescent protein as a reporter in rapid screening of antituberculosis compounds
in vitro

and in macrophages.
Biochem. Biophys
.
Res. Commun
..
253
, 1998, 431.


52.

Chaturvedi, V., Srivastava A., Gupta, H.P. and Sr
ivastava, B.S. :

Protective

antigens
of

Mycobacterium habana

are distributed between

peripheral and

integral
compartments of plasma membrane: a study in

experimental

tuberculosis of
mouse.
Vaccine

17
, 1999, 2882.


53.

Srivastava, R. and Srivastava, B.S.: Tuberculosis vaccines.
IDrugs
,
3
, 2000,

408.


54.

Deb, D.K., Srivastava, K.K., Srivastava, R. and Srivastava, B.S. :

Bioluminescent
Mycobacterium aurum

expressing firefly luciferase for

rapid and high throughput

9

screenin
g of antimycobacterial drugs
in vitro


and in infected macrophages.
Biochem. Biophys. Res. Comm. 279
, 2000,

457.


55.

Srivastava, R. and Srivastava, B.S. : Tuberculosis vaccines: a critical role


for T
-
cells.


Curr. Opinion Anti
-
inflammatory & Immunomodulator
y


Investigational Drugs 2
(2
), 2000, 100.

56.

Pathak R., Pant, C. S., Shaw, a.K., Bhaduri, A. P., Gaikwad, A. K., Sinha, S., Srivastava,
A., Srivastava, K. K., Chaturvedi, V., Srivastava, R., Srivastava, B.S. : Baylis
-
Hillman
Reaction: Convenient ascending
synthetesis and biological evaluation of acyclic
deoxy monosaceharides as potential antimycobacterial agents
. Biorg. Med. Chem.
10,

2002, 3187.


57.

Pathak, R., Shaw, A.K., Bhaduri, A.P., Chandrasekhar, K.V.G., Srivastava, A.,
Srivastava, K. K., Chatturvedi, V
., Srivastava, R. , Srivastava, B.S., Arora, S. and Sinha,
S. : Higher acyclic nitrogen containing deoxy sugar derivatives: a new lead in the
generation of antimycobacterial chemotherapeutics
. Biorg.

Med. Chem. 10
, 2002,
1695.


58.

Tripathi, R.P., Tripathi, R.
, Tiwari, V.K., Bala, L., Sinha, S., Srivastava, A., Srivastava,
R. and Sivastava, B. S. : Synthesis of glycosylated B
-
amino acids as new class of
antitubercular agents.
Eur. J. Med. Chem.


37
, 2002,


773.


59.

Deb, D.K.,


Dahiya, P., Srivastava, K. K., Sriva
stava. R. and Srivastava, B.S. : Selective
identification of new therapeutic targets of
Mycobacterium tuberculosis

by IVIAT
approach
. Tuberculosis

82
, 2002, 175.

60.

Prem Raj, P., Srivastava, S., Jain, S.K., Srivastava, B. S. and Srivastava, R. : Protection
by

live
Mycobacterium habana

cvaccine against
Mycobacterium

tuberculosis

H37Rv
challenge in mice.
Indian J.


Med. Res. 117
, 2003, 139.

61.

Tewari, N., Tiwari, V.K., Misra, R.C., Tripathi, R.P., Srivastava, A.K., Ahmed, R.,
Srivastava, R. and Srivastava, B.S. : S
ynthesis and bioevaluation of glycosyl ureas as a
-
glucosidase inhibitors and antituberculosis agents.
Bioorg. Med. Chem. 11,
2003,
2911.


10

62.

Katiyar, D.,


Tiwari, V. K., Tripathi, R. P.,


Srivastava, A.,


Chaturvedi, V.,


Srivastava,
R. and Srivastava, B. S.

: Synthesis an antimycobacterial activity of 3,5
-
disubstituted
thiadiazine thiones.
Bioorg. Med. Chem
.
11
, 2003, 4369.

63.

Mishra, A., Srivastava, R., Pruzzo, C. and Srivastava, B. S. : Mutation in
tcp
R gene
(Vc0832) of
Vibrio cholerae

O1 causes loss of toler
ance to high osmolarity and
affects colonization and virulence in infant mice.
J. Med. Microbiol. 52
, 2003, 1.


64.

Tiwari, V.K., Tripathi, R.P., Chaturvedi, V., Srivastava, A., Sinha, S., Srivastava,
R.Srivastava, B.S. Combinatorial and conventional synthesis

of glycohybrid molecules
as new class of antitubercular agents. Med. Chem. Res. 12, 2003, 394.

65.

Gautam Panda, Shagufta , Jitendra Kumar Mishra, Vinita Chaturvedi, Anil K.
Srivastava,
Ranjana Srivastava

and Brahm S. Srivastava 2004.
Diaryloxy methano
phenan
threnes: a new class of antituberculosis agents..
Biorg. Med. Chem.

12 . 5269 .

66.

Synthesis of Galactopyranosyl Amino Alcohols as a new class of Antitubercular and
antifungal agents.
N. Tewari,

V. K. Tiwari,

R. P. Tripathi
,

A. Gaikwad, S. Sinha, P. K.
Shukla, R. Srivastava and B. S. Srivastava,
Bio. Med. Chem
.
Lett.
,
2004
,
12
, 329.

67.

D. Katiyar, V.K. Tiwari, N. Tiwari, S.S. Verma, S. Sinha, A. Gaikwad, A. Srivastava, V.,
Chaturvedi, R. Srivastava, B.S. Srivastava and R.P. Tripa
thi 2005.
Synthesis of
antimycobacterial activity of Glosylated Aminesand Amino Alcohols..


Eur. J. Med.
Chem
.40, 351.

68.

M. Zampini, C. Pruzzo, V.P. Bondre, R. Tarsi, M. Cosmo, A. Bacciaglia, A. Chabbra,
R.
Srivastava
, B.S. Srivastava.. 2005.
Vibrio cholera
e

persistence in aquatic environments
and colonization of intestinal cells: involvement of a common adhesion mechanism.


FEMS Microbiol. Lett
. 244 , 267.

69.

Synthesis and antitubercular activities of bis
-
glycosylated diamino alcohols.

R.P.
Tripathi, V. K. Tiwari, N. Tewari, D. Katiyar, N. Saxena, S. Sinha, A. Gaikwad, A. K.
Srivastava, V. Chaturvedi, Y. K. Manju, R. Srivastava, B. S. Srivastava.
Bioorg Med
Chem
.,
2005,
13
, 5668.

70.

Ranjana Srivastava
, D. Kumar, M.N. Waskar, M. Sharma, V.M
. Katoch and B.S.
Srivastava.
2006. Identification of a repetitive sequence belonging to a PPE gene of

11

Mycobacterium tuberculosis

and its use in diagnosis of tuberculosis .
J. Med.
Microbiol
. 55, 1071
.


71.

Ravi

K.

Asthana
,
, Arunima

Srivastava, Akhilesh

P.

Sin
gh, Deepali, Sureshwar


P.

Singh,
Gopal

Nath,
Ranjana

Srivastava

and Brahm

S.

Srivastava. 2006. Identification of an
antimicrobial entity from the cyanobacterium
Fischerella

sp. isolated from bark of
Azadirachta indica

(Neem) tree .
J. Applied Phycology
18

(1), 33
.


72.

P. Akhtar, S. Srivastava, Anil Srivastava, Mukesh Srivastava,


Brahm S. Srivastava,
Ranjana Srivastava

2006.


Rv3303c of
Mycobacterium tuberculosis

protects tubercle
bacilli against oxidative stress
in vivo

and


contributes to virulence in mice.
Microbes
and infection

8, 2855
-
2862.


73.

Srivastava, V., C. Rouanet, R. Srivastava, B. Ramalingam, C. Locht and B.S. Srivastava.
2007. Macrophage specific
M. tuberculosis

genes identified by green fluorescent
protein an
d kanamycin resistance selection.
Microbiology

153 (Pt 3) 659
-
66. 48.


74.

Saquib, M., Manish K. Gupta, Ram Sagar, Yenamandra S. Prabhakar

, Arun


K. Shaw,

Rishi Kumar, Prakash R. Maulik, Anil Gaikwad, Sudhir Sinha, Anil K. Srivastava, Vinita
Chaturvedi, Ranja
na Srivastava and Brahm S. Srivastava..2007. C
-
3 Alkyl /Arylalkyl
-
2,3
-
dideoxy hex
-
2
-
enopyranosides as antitubercular agents: Synthesis, Biological
Evaluation and QSAR study
.

J. Med. Chem
., 2007,
50
, 2942.

75.

V. Srivastava,
A. Jain, B.S. Srivastava, R. Srivastava. 2008.

Selection of genes of
Mycobacterium tuberculosis

upregulated duri
ng residence in lungs of infected mice.
Tuberculosis (Edinb).

88 (3) 171.

76.

A. Saxena, V. Srivasrtava, R. Srivastava, B.S. Srivastava
. 2008
.
Identification of genes
of
Mycobacterium tuberculosis

upregulated during anaerobic persistence by
fluorescence and k
anamycin resistance selection.
Tuberculosis (Edinb)
, 88 (6) 518.

77.

R.P.S. Parti, R. Shrivastava, S. Srivastava, A.R. Subramanian, R. Roy, B.S. Srivastava,
R. Srivastava.
2008
. .
A transposon insertion mutant of
Mycobacterium fortuitum

attenuated in virulence and persistence in a murine infection model that is

12

complemented by Rv3291c of
Mycobacterium tuberculosis.

Microbial Pathogenesis
.
45(5
-
6), 370.

78.

A. Gupta, S. Bhakta, S. Kundu, M. Gupta, B.S. Srivastava, R. Srivastava.
2009.

Fast
-
growing, non
-
infectious and intracellularly surviving drug
-
resistant
Mycobacterium
aurum
: a model for
high
-
throughput antituberculosis drug screening.
J. Antimicrob.
Chemotherapy

64(4), 774.

79.

J. Kundu, R. Mazumdar, R. Srivastava, B.S. Srivastava.
2009
.
Intranasal
immunization with recombinant toxin
-
coregulated pilus and cholera toxin B subunit
protects r
abbits against
Vibrio cholerae

01 challenge.
Fems Immunol

Med
Microbiol
. 56(2), 179.

80.

R.K.
Asthana
, A.
Deepal
i, M.K.
Tripathi
, A.
Srivastava
, A.P.
Singh
, S.P.
Sing
h
, G.
Nath
,
R.
Srivastava
, B.S.
Srivastava.
2009
.
Isolation and identification of a new antibacterial
entity from the Antarctic cyanobacterium Nostoc CCC 537.

Journal of Applied
Phycology

21(1), 81.

81.

R. K. Gupta, B.S. Srivastava, R. Srivastava.
2010.

Comparative expression analysis of
rpf
-
like genes of
Mycobacterium tuberculosis

H37Rv under different physiological
stress and growth conditions.

Microbiology,

156

, 2714.

82.

Singh VK, Srivastava V, Singh V, Rastogi N, Roy R, Shaw AK, Dwivedi AK, Srivastava R,
Srivastava BS.
2011
.
Overexpression of Rv3097c in Mycobacterium bovis BCG
abolished

the efficacy of BCG vaccine to protect against Mycobacterium tuberculosis
infection in mice.
Vaccine
.
2011
;29(29
-
30):4754
-
60.

83.

Saquib M, Husain I, Sharma S, Yadav G, Singh VK, Sharma SK, Shah P, Siddiqi MI,
Kumar B, Lal J, Jain GK, Srivastava BS, Srivasta
va R, Shaw AK.
2011
.
2,3
-
dideoxy hex
-
2
-
enopyranosid
-
4
-
uloses as promising new anti
-
tubercular agents: design, synthesis,
biological evaluation and SAR studies.
Eur J Med Chem. 2011

46(6):2217
-
23.

84.

S
ingh V, Chandra D, Srivastava BS, Srivastava R
2011
.
Biochemical and transcription
analysis of acetohydroxyacid synthase isoforms in Mycobacterium tuberculosis
identifies these enzymes as potenti
al targets for drug development.
Microbiology.
;157(Pt 1):29
-
37.


13

85.

Singh V, Chandra D, Srivastava BS, Srivastava R
2011
.
Downregulation of Rv0189c,
encoding a dihydroxyacid dehydratase, affects gr
owth of Mycobacterium tuberculosis
in vitro and in mice.

Microbiology.
;157(Pt 1):38
-
46.

86.

Sharma SK, Moe TS, Srivastava R, Chandra D, Srivastava BS. 2011.
Functional
characterization of VC1929
of Vibrio cholerae El Tor: role in mannose
-
sensitive
haemagglutination, virulence and utilization of sialic acid.

Microbiology
.;157(Pt
11):3180
-
6.

87.

Kashyap VK, Gupta RK, Shrivastava R, Srivastava BS, Srivastava R, Parai MK, Singh P, Bera
S, Panda G
. 2012.

In vivo activity of thiophene
-
containing trisubstituted methanes
against acute and persistent infection of non
-
tubercular
M
ycobacterium fortuitum

in a
murine infection model.

J Antimicrob Chemot
her
. ;67(5):1188
-
97.


List of Patents:



International Patents granted

: 5


Mycobacterium tuberculosis

specific


DNA


fragment

US Patent no. 6,242,585 B1; 05. 06. 2001

Ranjana Srivastava
,
Deepak Kumar, B.S. Srivastava


Mycobacterium tuberculosis

specific


DNA


fragment

US Patent no. 6114514; 05. 09. 2000

Ranjana Srivastava
,
Deepak Kumar, B.S. Srivastava


Mycobacterium tuberculosis

specific


DNA


fragment

European Patent EP0945462; 08/02/2006

Germany, England, France and Netherland

Ranjana Sri
vastava
,
Deepak Kumar, B.S. Srivastava


Antitubercular extracts of
Salicornia brachiata


14

US Patent 20070020351; 25.1.2007

Meena Rajnikant Rathod
,
Bhupendra Dhanvantrai Shethia
,
Jayant Batukral Pandya
,
Pushpito Kumar Ghosh
,
Prakash Jagivanbhal Dodia
,
Brahm S. Srivastava
,
Ranjana
Srivastava
,
Anil Sri
vastava
,
Vinita Chaturvedi
,
Mariappanadar Vairamani


Herbal extracts of
Salicornia

species, process of preparation thereof, use thereof

against tuberculosis preparation thereof, use thereof against tuberculosis

US Patent 20070248704; 25.10.2007

Meena Rajnika Rathod
,
Bhupendra D. Shethia
, Jayant B.Pandya,
Pushpito K. Ghosh
,
Prakash J. Dodia
,
Brahm S. Srivastava
,
Ranjana Srivastava
,
Anil Srivastava
,
Vinita
Cha
turvedi
.



National Patent

: 22