IST 480: MOLECULAR DIOAGNOSTICS - MU BERT

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IST 241 Introduction to DNA Cloning

(
4
Credits)








FALL
2011







COURSE OUTLINE


Professor:

Menashi Cohenford,
MT, Ph.D

Required Text:



Understanding DNA and Gene Cloning

4
th

Edition by Karla Drlica
and

Introduction to
Biotechnology 2
nd

Edition
by Thieman and Palladino



Phone:

304
-
696
-
2697

Office

Location
:

BBSC Room 241H

Office Hours







Class Hours:
Location:



Mondays

and
Fridays

9

a
m

-
12

p
m

and 2

pm
-
5
pm

Wednesdays
9

am
-
12

p
m

and 4 pm
-
5 pm




O
r by
appointment








Tuesdays and Thursdays

9:30 am
-

10:45

a
m


Wednesdays 5 pm to 7 pm




BBSC 125


Prerequisites:

E
-
Mail Address:

No
ne


cohenford@marshall.edu

IST 241 Catalog Description

Basic Molecular Genetics. Topics include DNA, RNA, Protein Structure and Function,
Microbiology, Genetics, Cell Biology, Gene Regulation, Molecular Biology Appl
ications in
agriculture, medicine and industry. Hands
-
on DNA cloning lab.

Grades:

Your grade will be calculated as follows:






Exam 1:





25
%


Exam II:





25
%






Final Exam:





25
%






Quizzes
:







1
5
%






Lab Reports




10%




Total




100%








Your final grade in the class will be measured as follows:


A:

90
-
100

B:

80
-
89

C:

70
-
79

D:

60
-
70

F:

Below
60

2


Exams:
The exams will be focused on the materials
presented in class. All lectures will
be in power p
oint
format
. The final exam will be cumulative. Each exam will be based on
multiple choice questions and descriptive essays. These essays may at times be thought
provoking and require you to apply learned concepts in simulated situations.
M
ake
-
up

exams
will not be allowed

unless
you
contact
me prior to the exam with

a valid excuse.


Quizzes:
Following each main topic there will be a
15
-
20 minute quiz. The quiz dates
will be announced to allow for adequate preparation. The quiz may vary in format and
may include both multiple choice and short answer questions.
Quizzes may not be
made up for any reason
.


Lab Reports:

Following
the completion of
each lab
,

students are required to submit a
report of their findings.
Every

report must include an abstract, an introduction, a section
detailing the materials and methods used

followed by
result, discussion

and a reference
section.
The
s
ubmission

date for each report will be announced in advance.



Attendance:
Student attendance and participation will be required. Punctual attendance
to lectures

and labs

will be considered in the final grade. For example, if a student with a
68 average ha
s a full attendance record and has actively participated, that student may
receive a grade of C for the course.


Students Requiring Special Needs
: Students with disabilities who require special
accommodations should refer to this link

http://www.marshall.edu/disabled

which
provides information for the educational and physical accessibility support at the
university.


Academic Integrity & Honor Code
: The honor code as defined in the student
handbook will be strictly enforced. Violations of this code may result in your receiving a
failing grade for the class, and other disciplinary actions by the Academic Dean.





















3




*
DATES

* CHAPTERS


August
2
2
nd

-
2
6
th




Aug

24
th

(lab)


Introduction to
Biotechnology

Powerpoint
1

Structure and function of DNA and RNA

Powerpoint II


No Actual Lab 1
st

Week


August
29
th

-

September
2
n
d




Aug 31
st


Structure and function of DNA and RNA

Powerpoint III

Structure and function of DNA and RNA Powerpoint IV


Lab
I
-

Nucleic Acid Extraction

From Plant Tissues


September
5
th
-
9
th










Sept 5th


Sept 7
th


Structure and function of DNA and RNA Powerpoint IV (Cont.)


Isolation, Purification and Properties of

DNA and RNA

Powerpoint V


DNA Replication in Prokaryotic and Eukaryotic Cells

Powerpoint VI


Labor Day Holiday

(Sept 5
th
)


Lab
II
-
GAPDH PCR


September 12
th
-
16
th





Sept
14th

DNA Replication in Prokaryotic and Eukaryotic Cells

Powerpoint VI

(Cont)

DNA
Replication in Prokaryotic and Eukaryotic Cells

Powerpoint VII


Lab III
-

GAPDH PCR


September
19
th

-
2
3
th






Sept 2
1st

RNA Structure and Function

Powerpoint VII

(Cont.)

RNA Structure and Function

Powerpoint VIII


Lab IV
-

Electrophoresis

September 2
6
th
-

30
th




Sept 29th





Sept 28
th


RNA Structure and Function

Powerpoint
VIII (cont)



Protein Synthesis

Powerpoint IX




Lab V
-

PCR Purification


4


October
3
rd

-
7
th






Oct 5
th


Protein Synthesis Powerpoint IX (cont)

Protein Synthesis

Powerpoint Lecture X


Exam #1:

Materials from Powerpoint Presentations

Plus Reading Materials Provided by Instructor


Lab V
I
-

Ligation



October 1
0
th
-
1
4
th




Oct 12
th


Protein Synthesis

Powerpoint

X (Cont.)

Regulation of Gene Expression

Powerpoint XI


Lab VII
-
Transformation


October 1
7
th
-
2
1
st






Oct 19th

Regulation of Gene Expression

Powerpoint XII



Lab VIII
-

Plasmid Purification

October 2
4
th

-
28
th






Oct
26
th


DNA Cloning
Lecture
Series



Powerpoint 1:
Restriction Enzymes

Powerpoint
2
: Introduction to
Cloning Vectors

Part I


Lab IX
-

DNA Sequencing

Oct 31
st

-
Nov 4th



Nov 2
nd

DNA Cloning
Lecture
Series


Powerpoint 2: Introduction to
Cloning Vectors

Part I (Cont.)

Powerpoint 3: Cloning Vectors
Part II

Powerpoint 4:
Polymerase Chain
Reaction


Lab X
-

Bioinformatics

November
7
th

-
1
1
th








Nov 9th

DNA Cloning
Lecture
Series



Powerpoint 4:
Polymerase Chain Reaction (Cont.)

Powerpoint 5:
DNA Libraries





Introduction to
Immunity and
the Immune System


Powerpoint XIII


Lab XI
-

Immunoa
ssays

November 1
4
th
-
18
th





Nov 18th



November
17
th




Introduction to
Immunity and the Immune System




Powerpoint

XIII

(Cont.)


Exam #
2
:

Materials from Powerpoint Presentations
Since 1
st

exam

Plus Reading Materials Provided by Instructor


No Lab Scheduled


5


November 2
1
st


-
2
5
th




Thanksgiving/Fall
Break


Nov
28
th



Dec

2
nd



Nov 30
th

Introduction to Immunological Methods

Powerpoint IV






No Lab Scheduled

Nov 30
th

-
Dec 6
th



Dead Weak




Last Class Day

(Dec 6
th
)




Date and Time To Be
Announced

Final Exam

Rm 125


*Denotes that t
his syllabus is presented as a
guide only and may be changed at any time by the instructor.