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FACULTY OF BIOSCIENCES AND
BIOENGINEERING

Page :

COURSE: SQG
2153
GENETIC ENGINEERING

Revision

0

Date of issue


JULY 2009

Last Amendment

JULY 2009

Edition

1

Procedure No

FBB/UG/SQG2153

PRE
-
REQUISITE

SQG 1143

EQUIVALENCE

NONE

CREDIT HOURS

3 Credits

Lecturers

E
-
Mail

Room No.

Phone No.

1.

Prof. Noor Aini Abdul Rashid

nooraini_nar@yahoo.com


DSI Level 3

30068

019
-
7282311

2.

Dr. Chan Giek Far

giekfar@fbb.utm.my

giekfar@utm.my


C08
-
313

32707


SYNOPSIS


This course encompasses the basic principles and techniques involved in molecular biology which will
enable students to apply these techniques in the genetic engineering laboratory. The introductory lecture
will expose students to genetic engineering and
its application in various sectors of the industry such as
agriculture, medical, pharmaceutical, environmental, etc. The following lectures will revolve around
techniques in genetic engineering, cloning of heterologous genes in the
Escherichia coli

host wh
ich will
include common procedures in molecular biology, enzymes important in molecular biology, plasmids and
bacteriophage as cloning vectors, gene libraries preparation and screening for gene of interest. Before
semester ends, students are to produce a g
roup poster relevant to genetic engineering as a form of team
-
working experience. Peer group evaluation is mandatory.

Some of the concepts taught will be applied in
this laboratory practical. Students will have the opportunity to hands on the isolation of
genomic DNA,
agarose gel electrophoresis, PCR amplification, DNA ligation, preparation of competent cells,
transformation, and lastly verify the clones of interest by plasmid DNA extraction and DNA restriction
digestion.



















PREPARED BY :

CERTIFIED BY :

Name :
Prof. Noor Aini Abdul Rashid



Signature


Date :
July 2009

Name :


Signature :


Date :

FACULTY OF BIOSCIENCES AND
BIOENGINEERING

Page :

COURSE: SQG
2153
GENETIC ENGINEERING

Revision

0

Date of issue


JULY 2009

Last Amendment

JULY 2009

Edition

1

Procedure No

FBB/UG/SQG2153

COURSE LEARNING OUTCOMES


By the end of the course, students should be able to :


No.

Course Learning
Outcomes

Programme

Learning
Outcome(s)
Addressed

Assessment
Methods

1.

d
escribe the techniques used in the isolation,
manipulation and cloning of heterologous DNA

PLO1(C2), PLO2(C2)

Q, T,
F
(t)

2.

d
iscriminate the
use of
different kinds of enzymes
and
vectors in

molecular biology

application

PLO1 (C4), PLO2(C4),
PLO3(C4)

Q, T,
F
(t)

3
.

a
pply prior and cumulative knowledge in the
construction of gene libraries to clone genes of
interest based on the resources available

PLO1(C3), PLO2(C3)

Q, T,
F
(t)

4
.

a
nalyze relevant
information and experimental data
in

genetic engineering

PLO1(C4), PLO3(C4),
PLO4 (CS1)

P, Pr

5

demonstrate understanding
in

genetic
engineering
techniques
effectively in

team
work activities

PLO1 (C3), PLO2 (C3
,
P
3

, A3
), PLO3 (C3)
,
PLO6
(TS1
, P
3

, A
3
)

LR
, PA


Note :
Q


Quiz; T


Test

; F
(t)



Final Exam (Theory); P


Poster assignment, Pr


Presentation;


PA


Peer assessment; HW


Homework; LR


Lab report





























FACULTY OF BIOSCIENCES AND
BIOENGINEERING

Page :

COURSE: SQG
2153
GENETIC ENGINEERING

Revision

0

Date of issue


JULY 2009

Last Amendment

JULY 2009

Edition

1

Procedure No

FBB/UG/SQG2153

STUDENT LEARNING TIME (SLT)


Teaching
and Learning Activities

Student Learning Time
(hours)

1.Face to Face Learning

1.

Lecturer
-
Centered Learning

2.

Lecture


42

1.

Student
-
Centered Learning (SCL)

1.

Practical/Tutorial

2.

Student
-
centered learning activities


21

9

2.Self
-
Directed Learning

2.

Non
-
face
-
to
-
face learning
or student
-
centered learning
(SCL) such as manual, assignment, module, e
-
Learning, etc.

9

3.

Revision

18

4.

Assessment Preparations

12

3.Formal Assessment

3.

Ongoing Assessment
(Quiz + Test + Poster +
Presentation, Lab report)

6

1.

Final Exam

3

Total (SLT)

120





TEACHING METHODOLOGY


Lectures shall emphasize on theories, followed by homework on examples and further applications to
problems of interest in Genetic Engineering.


Interactive learning shall be conducted to provide
opportunities for students to be in smaller groups and
work together to appreciate the theories given in lectures. Students shall discuss amongst themselves and
solve given problems in class on related topics.


Briefing sessions prior to experiments and
monitoring students in carrying out experiments.


Hands on experiments.





FACULTY OF BIOSCIENCES AND
BIOENGINEERING

Page :

COURSE: SQG
2153
GENETIC ENGINEERING

Revision

0

Date of issue


JULY 2009

Last Amendment

JULY 2009

Edition

1

Procedure No

FBB/UG/SQG2153

WEEKLY SCHEDULE


Lecture:

Week

Lecture

Topic / Content

1

1
-

3

Introduction to Genetic Engineering:


4.

Definition of genetic engineering

5.

Basic techniques in molecular biology (an
overview)

6.

Applications of genetic engineering

7.

Advances of genetic engineering in agriculture, medicine,
pharmaceutical

2

4
-
6

Techniques in Molecular Biology
:

8.

Techniques in the separation and detection of DNA (electrophoresis)

9.

Techniques in the
identification of DNA (blotting, probe labeling and
detecting)

3

7
-
9

Techniques in Molecular Biology
:

10.

Polymerase chain reaction

11.

DNA Sequencing

12.

Site directed mutagenesis

QUIZ
1

4

10
-
12

Techniques in
Molecular Biology
:

13.

Microarray


Enzymes for molecular biology:


14.

Host restriction and modification

15.

Restriction endonuclease and restriction modification


5



13
-
15


Enzymes for molecular biology:


16.

Classification of enzymes

17.

Types and mechanistic action of restriction enzymes

18.

Types and mechanistic action of modification enzymes (DNA
dependent DNA polymerases, RNA dependent RNA Polymerases and
DNA dependent RNA polymerases)


6

16
-
18

Enzymes for molecular biology:


19.

Enzymes that mend DNA and RNA backbone bonds

20.

Enzymes involved in synthesis of cDNA and efficient cDNA
synthesis


QUIZ 2

7

19
-
21

Cloning in
E. coli

-

plasmid as cloning vector

21.

Plasmids, its characteristics and purification

22.

Desirable properties of cloning vectors

23.

Cloning and screening mechanism

8

22
-
24

Cloning in
E. coli
-

plasmid as cloning vector


24.

Criteria, usage and examples of expression vector

25.

Shuttle and specialized vector

TEST 1


9



25
-
27


Bacteriophage as cloning and expression vector

26.

Bacteriophage and its life cycle, promoter and circuit control,

27.

Mechanism of regulation of types of vector DNA

28.

High level expression in

vector

29.

Packaging
in vitro

QUIZ 3


FACULTY OF BIOSCIENCES AND
BIOENGINEERING

Page :

COURSE: SQG
2153
GENETIC ENGINEERING

Revision

0

Date of issue


JULY 2009

Last Amendment

JULY 2009

Edition

1

Procedure No

FBB/UG/SQG2153

Week

Lecture

Topic / Content

10

28
-
30

Bacteriophage as cloning and expression vector

30.

Cosmid vectors

31.

Phasmid, phagemid

32.

EMBL, BAC, and YAC

11


31
-
3
3


DNA libraries and Screening for Recombinants

33.

Types of libraries (genomic and cDNA)

34.

Construction of genomic library

35.

Construction of cDNA library


QUIZ 4



12

MID SEMESTER BREAK

13

34
-
36

DNA libraries and Screening for Recombinants

36.

Screening of libraries

37.

Antibody screening

38.

Labeling of probes

39.

Hybridization and detection of recombinants

TEST 2


14


37
-
39

POSTER PRESENTATION AND EVALUATION


15

40
-
42

POSTER PRESENTATION AND
EVALUATION

16
-
18

REVISION WEEK AND FINAL EXAMINATION


Lab Schedule

Week

Hands on

Topic / Content

3

1
-

3

Introduction of lab safety and lab report writing.

4

4
-
6

Genomic DNA isolation.

5

7
-
9

Polymerase chain reaction.

6

10
-
12

Agarose gel electrophoresis and PCR clean
-
up.

7

13
-
15

Ligation to cloning vector.

8

16
-
18

Preparation of competent cell.

9

19
-
21

Transformation and blue/white screening.

10

22
-
24

Plasmid miniprep.

11

25
-
27

Restriction digestion and agarose gel
electrophoresis.

Peer assessment

12

MID SEMESTER BREAK

13
-
14

Submission of lab report




FACULTY OF BIOSCIENCES AND
BIOENGINEERING

Page :

COURSE: SQG
2153
GENETIC ENGINEERING

Revision

0

Date of issue


JULY 2009

Last Amendment

JULY 2009

Edition

1

Procedure No

FBB/UG/SQG2153

REFERENCES


1

Richard J. Reece (2004). Analysis of genes and Genomes: John Wiley and Sons Ltd, England.

2

Clark, D.P. and Russel L.D. (1997). Molecular Biology
Made Simple and Fun: Cache River
Press, USA.

3

Madigan, M., Martinko , J. and Parker, J. (1997). Biology of Microorganisms: Prentice Hall
International (UK) Ltd, England

4

Karp, G. 2002. Cell and Molecular Biology
, John Wiley and Sons, New York

5

Voet,
D. & Voet, J. 2004. Biochemistry. 3
rd

Ed. Wiley International Edition

6

Animation and movies on related topics found in my blog
profnoorainiabdulrashid.blogspot.com




GRADING


No.

Assessment

Number

% each

% total

Dates/Weeks

1.

Quizzes

4

2.5

10

3, 6,
9, 11

2.

Test I & II

2

15

30

8 & 13

3.

Group

Poster
Presentation

1

5

5

4, 10, 15

4

Final Exam

1

40

40

16
-

18

5

Lab report

1

10

10

14

6

Peer assessment

1

5

5


Overall Total

100




ATTENDANCE


The student should adhere to the rules of attendance as

stated in the University Academic Regulation :
-


Students must attend not less than 80% of lecture hours as required for the course.


The student is prohibited from attending any lecture and assessment activities upon failure to comply the
above requireme
nt. Zero mark will be given for the course.