Job description PHD-1 - Cell-Wall engineering - Interactions - Nutrim

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11 Δεκ 2012 (πριν από 4 χρόνια και 11 μήνες)

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C




Job description
PhD


PhD
-
fellow [1: Cell
-
Wall engineering]


Theme



GI Health


Project title



Molecular Interactions


Project leader:

Koen Venema




FTE

1.0


Tariff

PhD fellow/ AIO

Based on tariff scale TIFN

Promotor

Michiel Kleerebezem


Location

NI
ZO Food Research
(aanstelling bij WUR)


Supervisors

Michiel Kleerebezem

Peter Bron


Start date

Asap


Period

2011
-
2014

Contract for the PhD student
is initially for one year.

After this first year, a positive
evaluation of the PhD
student can lead to an
extension to a maximum of
four years in total.



Project specific requirements


Experience in:

Molecular Microbiology

Genetic engineering

Host
-
microbe interactions


Role Description


This project aims to decipher molecular host
-
microbe communication.

Th
e model microbe that will be predominantly be used in this work is
Lactobacillus plantarum

for which
the complete genome is known and various post
-
genomic technologies have been established.
However, also
Lactococcus lactis

will be employed as a amenable h
ost for genetic engineering and
heterologous gene expression.
Genetic engineering will be employed to modulate the cell
-
wall
properties of this microbe, which will be characterized in terms of their consequences for microbial
physiology, cell
-
wall biochemi
stry as well as signaling capacities with host cells. The latter aspects of
the task (biochemistry and signaling) will actively involve the PhD but will be performed in close
collaboration with two postdocs within the same project team.

The PhD will be in
volved in construction and characterization of mutant libraries in
L. plantarum
,
targeting cell
-
wall biosynthesis genes. Mutagenesis will also include development of genetic
complementation and overexpression strategies and cloning of homologous and hetero
logous genes
in the wild
-
type and mutant background. The PhD project will include molecular approaches to launch
natural competence in
L. plantarum
to enhance transformation efficiencies and facilitate effective
mutagenesis, as well as implementation of ra
ndom mutagenesis approaches.
The PhD will
characterize the effects of these mutations on general m
icrobial physiology
and will collaborate with
two postdocs and a technician to unravel cell
-
wall biochemistry and host
-
signaling of the mutant
collection.


C

Main Tasks and responsibilities


The PhD student is responsible for:



staying abreast of developments in his scientific discipline by keeping up with the scientific
literature in his field and visiting scientific meetings



generating ideas within the scope o
f the defined subject



supervising of research assistants and students, where appropriate



delivering results in line with the project plan milestones and deliverables



keeping up with the latest scientific developments and integrating these in the project



co
nverting data and results of the sub
-
project into overview reports



keeping traceable records of all results and analyses according to the lab journal
procedure



writing high
-
quality clearly written papers about the results obtained and getting these
publis
hed in high

impact scientific journals.



completing a thesis after 4 years.