Biotech Practice Questions - Chapter 09

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Bio 225 Chapter
9

Practice Questions


1.

Which of the following steps most closely represents the definition of recombinant DNA
technology?

A.

Use a restriction enzyme to cut the genome of an organism into pieces

B.

Use a restriction enzyme to cut plasmids

C.

Insert

the restriction fragments into the plasmids

D.

Insert the plasmids into cells

E.

Screen the cells to determine which contains the restriction fragment with the gene of
interest

F.

Allow the cells to reproduce

G.

Harvest the gene product


2.

Which of the following steps
most closely represents the definition of genetic engineering?

A.

Use a restriction enzyme to cut the genome of an organism into pieces

B.

Use a restriction enzyme to cut plasmids

C.

Insert the restriction fragments into the plasmids

D.

Insert the plasmids into cell
s

E.

Screen the cells to determine which contains the restriction fragment with the gene of
interest

F.

Allow the cells to reproduce

G.

Harvest the gene product


3.

Which of the following steps most closely represents the definition of cloning?

A.

Use a restriction enzym
e to cut the genome of an organism into pieces

B.

Use a restriction enzyme to cut plasmids

C.

Insert the restriction fragments into the plasmids

D.

Insert the plasmids into cells

E.

Screen the cells to determine which contains the restriction fragment with the gene
of
interest

F.

Allow the cells to reproduce

G.

Harvest the gene product


4.

Which of the following steps most closely represents the definition of biotechnology?

A.

Use a restriction enzyme to cut the genome of an organism into pieces

B.

Use a restriction enzyme to cut

plasmids

C.

Insert the restriction fragments into the plasmids

D.

Insert the plasmids into cells

E.

Screen the cells to determine which contains the restriction fragment with the gene of
interest

F.

Allow the cells to reproduce

G.

Harvest the gene product



5.

Which of th
e following steps is the first to use a vector?

A.

Use a restriction enzyme to cut the genome of an organism into pieces

B.

Use a restriction enzyme to cut plasmids

C.

Insert the restriction fragments into the plasmids

D.

Insert the plasmids into cells

E.

Screen the ce
lls to determine which contains the restriction fragment with the gene of
interest

F.

Allow the cells to reproduce

G.

Harvest the gene product


6.

What are restriction enzymes?

A.

Enzymes that only work in restricted areas of the cell

B.

Enzymes that are specific for pla
smids

C.

Enzymes that cut introns out of pre
-
mRNA

D.

Enzymes cut DNA molecules at specific recognition sites


7.

What are sticky ends?

A.

Ends of cut DNA that are even

B.

Ends of uncut chromosomal DNA

C.

Areas of restriction enzymes that bind to plasmids

D.

Areas of restrictio
n enzymes that recognize genomic DNA

E.

Ends of cut DNA that have overhangs


8.

The best description of a rule for using restriction enzymes to produce recombinant DNA
molecules is:

A.

Use a restriction enzyme that produces blunt ends

B.

Use as many different restrict
ion enzymes as possible

C.

Don’t use the same restriction enzyme on the genomic DNA that is used on the plasmid

D.

A restriction enzyme that produces sticky ends, used on both the genomic DNA and the
plasmid, will give the best chance of recombination


Use for Q
9 and 10

Enzyme

Recognition sequence

(
cuts at *
)

BamHI

G*GATCC

CCTAG*G

EcoRI

G*AATTC

CTTAA*G

HindIII

A*AGCTT

TTCGA*A

PstI

CTGCA*G

G*ACGTC


9.

Which of the following restriction enzymes makes sticky ends?

A.

BamHI

B.

EcoRI

C.

HindIII



D.

PstI



E.

All of the above

F.

None

of the above


10.

Which restriction enzyme would cut a strand of DNA with the
following
sequence
?



GGTACCGTGAATTCGAG

CCATGGCACTTAAGCTC


A.

Bam HI


B.

Eco RI


C.

HindIII



D.

Pst I





11.

The method of inserting foreign DNA into cells that relie
s on fusing bacterial cells that lack
cell walls is:

A.

Using a gene gun

B.

Microinjection

C.

Protoplast fusion

D.

Electroporation

E.

Transduction


12.

The method of inserting foreign DNA into cells that relies on forcing particles coated with
DNA across cell walls is:

A.

Using

a gene gun

B.

Microinjection

C.

Transformation

D.

Electroporation

E.

Transduction


13.

The method of inserting foreign DNA into cells by using a viral vector is:

A.

Using a gene gun

B.

Microinjection

C.

Transformation

D.

Electroporation

E.

Transduction


14.

A

method of getting plasmids int
o cells that don’t normally take up naked DNA is:

A.

Using a gene gun

B.

Microinjection

C.

Conjugation

D.

Electroporation

E.

Transduction


15.

If you want to clone a gene, what’s the first thing you have to do?

A.

Digest the DNA with restriction enzymes

B.

Cut your plasmid with re
striction enzymes

C.

Ligate the plasmid with a restriction fragment

D.

Incubate the cells you will transform with the recombinant plasmids

E.

Lyse the cells with the genomic DNA you’re interested in cloning


16.

What is a gene library?

A.

A computerized database of gene
sequences

B.

The entire sequence of a particular organism’s genome

C.

A set of synthetic cDNA molecules that represent all of the genes of a particular organism

D.

A bunch of clones containing all of the restriction fragments generated from a restriction
digest of
an organism’s genome


17.

What is synthetic DNA?

A.

DNA created by recombinant DNA technology

B.

DNA created by PCR

C.

cDNA

D.

DNA created using one organism’s genome for a template and another organism’s DNA
polymerase and nucleotides

E.

DNA created with a machine (DNA synt
hesizer)


18.

You want to clone some DNA, so you use a plasmid with an ampicillin resistance gene as a
vector. After transforming the bacteria you’ll be using to clone the DNA you streak them out
on media containing ampicillin. Colonies that contain the plas
mid can be identified by:

A.

Their blue color

B.

Their white color

C.

Replica plating

D.

Growth


19.

To determine whether or not a clone contains the specific gene of interest you could use:

A.

Replica plating

B.

Blue
-
white screening

C.

X
-
gal

D.

Ampicillin

E.

A probe


20.

To locate a specif
ic piece of DNA on a group of restriction fragments the probe must be:

A.

Identical to the gene of interest

B.

Radioactive

C.

Properly inserted into the cloning site on the plasmid vector

D.

Single stranded



21.

What do you need to make cDNA?

A.

A cloning vector

B.

An enzyme t
hat removes introns

C.

A template, some nucleotides, and Taq polymerase

D.

Reverse transcriptase

E.

None of the above


22.

What is the difference between cDNA and genomic DNA?

A.

cDNA has no codons

B.

cDNA has uracil instead of thymine

C.

cDNA is circular in eukaryotes

D.

cDNA has

no introns


23.

Which of the following is most problematic when using
E. coli

to produce human gene
products?

A.

E. coli

can’t excise introns to produce mRNA from eukayotic genes

B.

E. coli

doesn’t naturally secrete most gene products

C.

E. coli

DNA polymerase won’t c
opy human DNA

D.

E. coli

RNA polymerase won’t transcribe human DNA

E.

The product may be contaminated by endotoxin


24.

Genetic engineering can be used to produce:

A.

Subunit vaccines

B.

Hormones

C.

Disease resistance in plants

D.

Improved shelf life of agricultural products

E.

Al
l of the above


25.

Gene therapy uses genetic engineering to:

A.

Inhibit oncogene expression

B.

Eliminate copies of disease
-
causing genes

C.

Construct plants that produce their own insecticide

D.

Impart pesticide resistance to plants

E.

Treat diseases that result from defect
ive or missing genes


26.

You take the gene that codes for Bt toxin from
Bacillus thuringiensis



organism A
-

and put
it into the leaf colonizing organism
Pseudomonas fluorescens



organism B. The
Pseudomonas

colonizes a tomato plant


organism C. The res
ulting organism
(organism
C

after colonization)

is:

A.

Bacillus thuringiensis

B.

Pseudomonas fluorescens

C.

E. coli

D.

A plant x
Pseudomonas

hybrid

E.

A tomato plant


27.

You take the gene that codes for Bt toxin from
Bacillus thuringiensis



organism A
-

and put
it into th
e leaf colonizing organism
Pseudomonas fluorescens



organism B. The
Pseudomonas

colonizes a tomato plant


organism C. The resulting organism
(organism
B

with the Bt toxin gene)

has:

A.

A tomato gene

B.

An
E. coli

gene

C.

A
Bacillus

gene

D.

A tomato and a
Bacillu
s

gene

E.

No new gene



28.

The purpose of PCR is:

A.

To identify specific genes

B.

To make cDNA

C.

To make mRNA

D.

To make multiple copies of plasmid cloning vectors

E.

To make multiple copies of a desired piece of DNA enzymatically


29.

PCR makes DNA:

A.

By synthesizing individual

nucleotides

B.

Using
Thermus aquaticus

to synthesize new DNA molecules

C.

By using reverse transcriptase

D.

Without a living organism


30.

After 2 rounds of PCR how many new single stranded DNA molecules do you have?

A.

One

B.

Two

C.

Four

D.

Eight

E.

None of the above


31.

Southern blo
tting is used to:

A.

Identify specific mRNA molecules present in an organism

B.

Identify specific proteins present in an organism

C.

Identify clones with a specific gene

D.

Identify restriction fragments with a specific piece of DNA


32.

Which of the following is the fift
h step in a Southern blot?

A.

Lyse cells

B.

Do electrophoresis

C.

Transfer bands to nitrocellulose filter

D.

Develop film

E.

Expose film

F.

Hybridize with probe

G.

Restriction digest

Key

Bio 225 Chapter
9

Practice Questions


1

c

See definition

2

d

See definition

3

f

See def
inition

4

g

See definition

5

b

The plasmid is a vector

6

d


7

e


8

d


9

e


10

b


11

c

Protoplast fusion wasn’t choice C, there was no correct answer so I changed it.

12

a


13

e


14

d


15

e


16

d

C looks good but the cDNA molecules have to be i
n a vector and in cells

17

e


18

d

Only ampicillin resistant cells grow, only cells with plasmid are ampicillin resistant

19

e


20

d

It has to be complementary to the DNA of interest and single stranded so it can hybridize

21

d


22

d


23

e

Probably
most

problematic since you would have dealt with the other problems up front.

24

e


25

e

You put a working copy of the gene in to compensate for the defective or missing gene

26

e


27

c


28

e


29

d

B would be correct if it said …
Thermus aquaticus

DNA

polymerase


30

e

You’d have 8 total
single strands
and six
new

single strands (the other 2 were original template)

31

d


32

f