Effect of Phanerochaete chrysosporium fungus and the bacteria ...

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22 Φεβ 2013 (πριν από 4 χρόνια και 5 μήνες)

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Biodegradation activities of
Phanerochaete

chrysosporium

fungus and the bacteria
Pseudomonas
putida

and
Sphingomonas

macrogoltabidus

on
pretreated HDPE plastic

Anne Richards, Cara Broshkevitch,
Ong

Kim Yao,
and
Poh

Yong
Rui


HDPE plastic: used in food packaging, plastic bags, plastic
bottles, recycled plastic lumber, toiletry and liquid
containers, outdoor furniture, and piping


Takes over 100 years to degrade in a landfill
-
discarded
plastics filling up landfills (Sivan
,
2011)


Detrimental environmental effects:


Water and air cannot enter soil


deplete underground water
resource


Disrupts degradation of other substances


In the ocean, plastic chokes and entangles marine life


Background


Other ways of disposal can not be used:


Burning plastics at high temperatures produces
toxic irritants that are human carcinogens


Past research: biodegradation with bacteria or
fungi, UV radiation, and thermal radiation have
been separately investigated


All three organisms have been shown to degrade
polyethylene plastic separately


Background continued

Academy of Science

Hypothesis
:

The following factors affect
the rate of biodegradation of
HDPE plastic mass:


Ratios of different
microorganisms


P.
Chrysosporium

fungus


P.
Putida

bacteria


S.
Macrogoltabidus

bacteria

Hwa

Chong Institution

Hypothesis:

The following factors affect
the rate of biodegradation of
HDPE plastic mass:


Environmental conditions for
biodegradation


Exposure time to UV
radiation



Purpose

To determine the optimum treatment of
HDPE plastic for maximum biodegradation.

Academy of Science


Independent variables



Ratios of
P.
chrysosporium

fungus,
P.
putida

bacteria, and
S.
macrogoltabidus

bacteria


Dependent variable


Percent change in dry
mass of the HDPE
plastic samples


Amount of CO
2

gas
present


Hwa

Chong Institution


Independent variables


Environmental conditions for
biodegradation


Exposure time to UV radiation



Dependent variables


Percentage change in dry mass
of HDPE samples


Amount of CO
2

gas present


Temperature of contents of flask


Cell density of bacterial cultures

Variables:

Academy of Science

Culture Bacteria and Fungi

Pre
-
treat Plastic

Expose Plastic to Individual
Microorganisms

Expose Plastic to different
Ratios of 3 Microorganisms

Measure Dependent
Variables

Hwa

Chong Institution

Culture Bacteria

Prepare Soil
Conditions

Pre
-
treat Plastic

Expose Plastic to
Microorganisms

Measure Dependent
Variables

Procedure

Bacteria will be cultured in
culture test tubes with
nutrient broth

Fungi will be cultured in
Petri dishes in potato
dextrose agar

Microorganism Culture (AOS & HCI)

Erlenmeyer flask with
minimal media

Erlenmeyer flask with loamy soil
and distilled water

Prepare Soil Conditions (HCI)

Pre
-
treat HDPE Plastic (AOS & HCI)

HDPE Plastic
Grocery Bags

Exposed to
365nm UV
radiation for 96
hours

Cut into one
gram pieces

Exposed to
thermal radiation
in the oven at
115
°
C for 48
hours

Exposed to
365nm UV
radiation
for 72, 96,
120 hours

A

O
S

H
C
I

Cut into one
gram pieces

HDPE Plastic
Grocery Bags

Exposed to
thermal radiation
in the oven at
115
°
C for 48
hours

Academy of Science

Expose plastic to:


Individual
microorganisms


P.
putida


S.
macrogoltabidus


P.
chrysosporium


Different ratios of
the three
microorganisms

Hwa

Chong Institution

Expose plastic to:


P.
putida

in:


Minimal Media


Loamy soil +
Distilled
water

Expose Plastic to Microorganisms (AOS & HCI)

Will be used to measure:


Percentage change in dry mass of HDPE samples


Amount of CO
2

gas present


Temperature of contents of flask


Cell density of bacterial cultures


Measure Dependent Variables (AOS & HCI)

Thermometer

CO
2

Probe

Analytical Balance

Spectrophotometer

A
O
S

H
C

I

Aamer

Ali Shah (2007). Role of Microorganism in Biodegradation of Plastics. Retrieved October 30, 2011 from
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