Presentation_LTEM

Laboratory of Enzyme and Microbial

Technology

Research
Programme

Research Programme

Service and Facility Unit

Fermentation Technology

Enzyme Technology


Bioprocess Engineering


Strain Development and
Modification


Microscopy and Microanalysis
Techniques

Digestive Microbiology

Fermentation Technology

Microscopy and Microanalysis

Head of Laboratory

Assoc. Prof. Dr. Arbakariya Ariff

ORGANISATION STRUCTURE

Research
Programme

Research
Programme

Programme

Coordinator
:

Prof
.

Dr
.

Mohamed

Ismail

Abdul

Karim



Research

Projects
:

i.
Development

of

yeast

fermentation

for

the

production

of

biomass,

gluthathione

and

amino

acids
.

ii.
Development

of

productive

fermentation

technique

for

the

cultivation

of

beneficial

microorganisms

for

aquaculture

industry
.


iii.
CG tase and pullulanase production by local strains.

iv.
Production

of

microbial

PUFA
.

v.
Development

of

mass

cultivation

of

aquatic

yeast

isolated

from

local

sources
.




1. Fermentation Technology

Research
Programme

2
.

Bioprocess

Engineering


Programme

Coordinator
:

Assoc
.

Prof
.

Dr
.

Arbakariya

Ariff


Research

Projects
:


i.
Freeze

drying

of

probiotic

microorganisms
.

ii.
Biosorption

of

heavy

metals

by

microbial

biomass
.


iii.
Purification

of

recombinant

protein
.

iv.
Purification,

packaging

and

application

of

lipase

enzyme
.

v.
Bioreactor

system

for

animal

cell

suspension

culture

vi.
Bioreactor

system

for

the

cultivation

of

plant

cell

in

suspension
.


Research
Programme

3
.

Strain

Modification

and

Development



Programme

Coordinator
:

Assoc
.

Prof
.

Dr
.

Raha

Abd

Rahim



Research

Projects


i.
Systematic

deletion

of

hypothetical

open

reading

frame

(ORF)

and

protein

localization

for

elucidation

of

function

of

Saccharomyces

cerevisae
.

ii.
Screening

and

isolation

of

microorganisms

that

have

potential

for

industrial

use

from

local

sources
.


iii.
Development

of

recombinant

yeast,

Saccharomyces

cerevisae
,

capable

to

be

used

in

direct

fermentation

of

starches

to

ethanol
.


iv.
Improvement

of

fungal

strain

for

fermentation

process

via

monospores

isolation

technique
.



Research
Programme

4
.

Enzyme

Technology


Programme

Coordinator
:

Prof
.

Dr
.

Mahiran

Basri



Research

Projects

i.
Scale
-
up

of

lipase

enzyme

production

using

recombinant

strain
.

ii.
Enzymatic

synthesis

of

wax

ester
.

iii.
Biosensor
.

iv.
Protein

structure

and

function

Research
Programme

5
.

Microscopy

and

Microanalysis

Techniques


Programme

Coordinator
:

Assoc
.

Prof
.

Dr
.

Fauziah

Othman


Research

Projects

1.
In
-
vitro

effect

of

Newcastle

disease

virus

infection

in

breast

cancer

cells
.

i.
In
-
vivo

cytotoxicity

effects

on

breast

cancer

cells
.

ii.
Transmission

microscopy,

confocal

microscopy,

light

microscopy

and

elemental

analysis

using

energy

dispersive

x
-
ray

microanalysis

during

hepatocarcinogenesis

treated

with

Henna

and

Strobilanthus
.

iii.
Molecular

study

of

anti
-
cancer

properties

of

Henna

and

Stobilanthus
.

iv.
Cytotoxicity

and

oncilyic

effect

of

Henna

and

Strobilanthus

Research
Programme

6
.

Digestive

Microbiology


Programme

Coordinator
:

Assoc
.

Prof
.

Dr
.

Norhani

Abdullah


Research Projects:


i.
Molecular

study

of

several

Lactobacillus

strains

used

as

probiotic

in

poultry
.

ii.
The efficiency of beta glucanase produced by Lactobacillus
probiotics on growth performance of broiler.

iii.
The production of avian egg yolk antibody (IgY) against
Salmonella and
E.coli
.

iv.
Large scale production of probiotic microorganisms for chicken.

Service

and

Facility

Units


1.
Fermentation

Technology


Unit
:

Coordinator
:

Assoc
.

Prof
.

Dr
.

Arbakariya

Ariff

The

main

objective

of

the

unit

is

to

provide

scale
-
up

facilities

to

researchers
.

It

is

an

avenue

to

commercialize

potential

research

findings

and

also

to

act

as

a

platform

for

the

private

companies

to

get

involve

in

biotechnology

business
.


Fermentation

Various

sizes

of

bioreactor

(
2
,

10
,

50
,

100

and

200

L)

equipped

with

accessories

and

computer

control

system,

which

enabled

it

to

be

operated

in

different

operation

modes

(batch,

continuous

and

fed
-
batch)

are

available

at

FTU
.


Research
Programme

Downstream Processing


•

Continuous

flow

centrifuges,

homogenizer,

normal

flow

filtration

system,

tangential

flow

filtration

system,

pilot

scale

rotary

evaporator,

pilot

chromatography

system

and

freeze

dryer
.



Research
Programme

Analytical

•
High Performance Liquid Chromatography, Gas Chromatography,
Microkjedhal, Glucose analyser, Rheometer, Gel Documentation
system.

Research
Programme

Packaging Equipment


•
Semi
-
automatic volumetric and gravity filling machine, Horizontal
sachet packaging machine, capping and labelling machine



Research
Programme

2
.

Microscopy

and

Microanalysis

Unit


Unit

Coordinator
:

Assoc
.

Prof
.

Dr
.

Fauziah

Othman


•
Microscopy

and

Microanalysis

Unit

(MMU)

provides

service

and

facilities

for

research

and

training

in

the

field

of

electron

microscopy,

light

microscopy,

confocal

microscopy

and

microanalysis

to

the

scientists

and

researchers

from

universities,

research

institutes

and

private

companies
.


Research
Programme