Food research and molecular biotechnology at FCUB - FCUB-ERA

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20 Φεβ 2013 (πριν από 4 χρόνια και 3 μήνες)

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Food research and
molecular
biotechnology at
FCUB

Faculty of Chemistry,

University of Belgrade,

Serbia

FCUB


Founded in 1988 by the Government of Serbia, following division of
the Faculty of Natural Sciences into several independent faculties


Non
-
profit entity


Education of chemists, biochemists and chemistry teachers;
research


92 employed researchers, 42 teachers


From 2009 it has its own Innovation Center with 25 employed
researchers

Resources


National Projects (MNTR)


National Investment fund


Own resources (international students)


International Projects


Research projects: NATO SFP


Mobility schemes: COST 928, DAAD


Education support: TEMPUS, WUS Austria

Facilities


Starting from 2005 trough various funding
resources, up to 1 mEuro was put into
upgrading research facilities at the FCUB


Protein analysis (PA)


Structural Instrumental Analysis (SIA)


Molecular Biology and Biotechnology (MBB)


Cell Culture facilities (CC)

National Investment Fund and WUS Austria grants


2003


Protein analysis


2004


Laboratory for Molecular Biology


2007


Structural instrumental analysis



Cell culture laboratory


2009


Laboratory for Molecular Biotechnology

Facilities for protein isolation,
purification and analysis


Ultracentrifuge Sorwall 80,


ÄKTA purifier HPLC,


1D and 2D PAGE protein analysis, semy
-
dry and wet electroblotters,


ELISA microplate reader and washer,


Waters Breeze HPLC with electrochemical detection,


Gel Documentation System,


Eppendorf Centrifuges


microcentrifuges with cooling,


System for Ultra pure water,


Deep Freezers (
-
80 oC),


Lyophylizer.

Molecular Biotechnology and
Enzymology facilities


Master Cyclers with gradient system,


Electroporators,


Autoclave,


Incubators and shakers,


Illuminators,


Microplate readers,


Centrifuges with cooling,


Low pressure LC system,


Deep Freezers (
-
80 oC),


Analytical and Preparative Electrophoresis systems for DNA analysis,


inNO Nitric Oxide measuring System, Innovative Instruments Inc (USA)
,
inO2 Dissolved Oxygen meter, Innovative Instruments Inc (USA).

Cell culture facilities


Laminar flows,


CO2 incubators,


Centrifuges with cooling,


Microscopes and inverted microscopes,


Deep Freezers (
-
80 oC),


Liquid nitrogen containers,


Flow cytometer FACSCalibur (BD Biosciences).

Structural instrumental analysis
facilities


Superconducting Fourier
-
Transform NMR Spectrometer AVANCE


500 MHz
,


Parr Hydrogenization system up to 200 barr,



System for synthesis and purification of small physiologically active
molecules consisting of glove box, microwave reactor and Biotage
chromatography system
,


GC
-
MS,


Elemental analysis system,


UV/VIS spectrophotometer,



FT
-
IR Spectrometer, Polarimeter,


HPLC with UV/VIS detection
,


HPLC with electrochemical detection.

Strategic plan of the FCUB development for the 5
-
year period involves following:



-

Food safety and quality
: Evaluation and standardization of food allergenic extracts
(peanuts, cow’s milk, fruit and vegetables) at the molecular level. Development of
cutting
-
edge methods for trace allergen contaminants determination in food matrix
(MS
-
based and multiplex technologies based on flow
-
cytometry).



-

Food biotechnology and enzymology
: Development of new enzyme tools for food
industry, based on production, preparation and stabilization of enzymes (protease,
glycohydrolases, lipases) by techniques of molecular biotechnology. Development of
new recombinant allergens for the purposes of allergy diagnosis improvement.
Translation of research to industry and SME.



-

Food for health
: Development of new prebiotic and probiotic products for healthy
newborns
-

In vitro

infant formulae
.

New nutraceuticals development with organically
bounded trace elements. Healthy diet: methods development for organically bounded
oligo
-

and micro
-
elements in food. Impact of bioprocessing of food on health effects
of food, digestion, allergenicity and bioactive compounds release.Translation of
research to industry and SME.

Research groups presentation


Department of Biochemistry


Department of Analytical Chemistry


Research group 1 (RG1):


Group leader: Tanja Cirkovic Velickovic


PhD students: Jelena Radosavljevic, Luka Mihajlovic, Jana Ognjenovic, Marija Stojadinovic


MS students: Jasna Bojcevski



Main area of interest:




Food bioprocessing




Health effects of food




Food safety, including food allergens



Ongoing projects:


Microwave and ultrasound enhanced action of cross
-
linking enzymes on proteinaceous
substrates


Effects of polysaccharide food matrices and bioprocessing on the protein digestion by
gastrointestinal enzymes


Food allergens and hypoallergenic foods by chemical and enzymatic means


New quantification methods for food allergen detection, including multiplex technology


Recombinant food allergens for food allergy diagnosis


Research group 2 (RG2):


Group leader:
Marija

Gavrovic
-
Jankulovic


Senior researchers: Milan
Nikolic


PhD students:
Milica

Popovic
,
Milica

Grozdanovic
,
Rajna

Dimitrijevic
, Mohamed Salem
Alzwam

Abugren


MS students: Igor
Macinkovic
,
Draidi

Abuajila

Rogaia


Main area of interest:




Food nutrients with
immunomodulatory

potential




Food safety including food allergens




Food
probiotics




Health effects of food


Ongoing projects
:


Modulation of human immune response by food nutrients


Production of food proteins by recombinant DNA technology


Identification of novel food allergens & development of a monoclonal
-
antibody based assay
for detection of food allergens


Microbial production of novel food
probiotics


Influence of the different dietary patterns on red blood cell lipids



Research group 3 (RG3):


Group leaders
: Ratko Jankov


PhD students: Dusan Velickovic, Aleksandra Dimitrijevic, Milena Rikalovic, Ziyad Tantoush




Main area of interest:




Enzyme isolation and characterization




Enzyme stabilization and application in nonaqueos systems



Ongoing projects:


Isolation, immobilization and stabilization of glucosidases (glucoamylases, α
-
glucosidases,
lipases)


Synthesis of physiologycally active glycosides


Application of glycosidases and lipases in synthesys of glycolipids and glycosides.


Food bioprocessing by oxidases



Research group 4 (RG4):


Group leader:
Zoran

Vujcic


PhD students:
Uros

Andjelkovic
,
Biljana

Dojcinov
, Aleksandra
Milovanovic
, Nikola
Loncar




Main area of interest:




Food
bioprocessing




Food safety, including food contaminants




Food utilization




A
nimal

feedstuffs




Ongoing projects:


Enzyme immobilization techniques (using natural, synthetic and composed materials) and
their use in continual substrate
biotransformations

(bioreactors) and biosensors.


New quantification methods for food enzyme and contaminant detection.


Chemical modifications of proteins and nucleic acids; examination of effects of modification
on the structure and function of proteins and nucleic acids.


Food chemistry and biochemistry.
Flavours
. Connection between chemistry of natural
products with food chemistry and natural food contaminants.


Purification and characterization of enzymes from various food sources. Examination of
enzyme structures and their interactions with other proteins (antibodies,
lectins
, enzymes
and enzyme inhibitors) and nucleic acids.


Influence of food ingredients on the digestive enzyme expression and food utilization with
midgut

of
Morimus

funereus

larvae as a model system.



Research group 5 (RG5):


Group leader:
Miroslav

Vrvic


Senior researchers:
Ljuba

Mandic
,
Vesna

Jovanovic


PhD students: Mila
Ilic
,
Nikoleta

Lugonja
, Olga
Martinov
, Vladimir
Beskoski
,
Srdjan

Miletic
,
Jelena

Milic
,
Jelena

Acimovic,Tamara

Lazarevic
-
Pasti



Main area of interest:




Food biotechnology




Food supplements




Food microbiology




Food safety control




Health effects of food




Ongoing projects:


Developing of new
prebiotic

and
probiotic

products for healthy newborns
-

In vitro

examination of infant formulae
.


New
nutraceuticals



organically bounded trace elements: chromium yeast


Examination of organically bounded
oligo
-

and micro
-
elements in food


healthy diet


Characterization of advanced
glycation

end products.

Protein
thiol

group modification with
methylglyoxal

generated in diabetes.


Enzymes and modified proteins


diagnostic markers of diabetic complications and chronic
renal failure. Development of methods for their determinations.



Research group 6 (RG6):


Group leader:
Dusana

Milojkovic
-
Opsenica


Senior researchers:
Zivoslav

Tesic
,
Maja

Natic
,
Dragan

Manojlovic
,
Jelena

Mutic


PhD students:
Jelena

Trifkovic
,
Filip

Andric, Kristina
Lazarevic
,
Dragana

Dabic
, Ivan
Andjelkovic




Main area of interest
:


Determination of trace elements in food by ICP and AAS.


Trace analysis of pesticides residues by HPLC
-
UV detection.


Chromatographic analysis (HPTLC, HPLC, IC) of food additives.


Water quality control (analysis of anions) by ion chromatography.


Application of bioassays for screening purposes of POPs chemicals in food.




Ongoing projects
:


New method development and method assessment for quantification of trace elements and
multiresidue

analysis of pesticides in foodstuff.


Food origin and authenticity, development and application of novel
chemometrics

approach.


Macroporous ultramicroelectrode arrays for environmental analysis of metal cations
ultratraces.


Use of ELISA tests based on formation of ArH receptors

dioxin like compounds complex in
screening for POPs chemicals in foodstuff.


Food biotechnology and
enzymology
Health effects of food
Food quality and safety
RG3 RG4
RG5
RG2
RG6
RG1
Selected publications: Health
effects of food


Gavrovic
-
Jankulovic, M., Grozdanovic, M. (in press 2010)
Application of Plant
-
Derived Food Lectins in
Proteoglycomics and Immunomodulation.

Handbook of Nutritional Biochemistry: Genomics, Metabolomics and
Food Supply. Ed.: Sondre Haugen and Simen Meijer ISBN: 978
-
1
-
60741
-
916
-
7


Nikolic, M., Medic Ristic, D., Stanic, D., Postic, M., Arsic, A., Niketic, V. (2008).
Dietary lipid intake influences
the level of cholesterol bound to haemoglobin in human erythrocytes.
European Journal of Nutrition. 47(3),
123
-
130. (
RG2
)


Gorjanovic S., Beljanski M., Gavrovic
-
Jankulovic, M., Gojgic
-
Cvijovic G., Pavlovic M., Bejosano F. (2007)
Antimicrobial activity of malting barley grain thaumatin
-
like protein isoforms S and R
,
Journal of the
Institute of Brewing, 113, 206
-
212. (
RG5
)


Gavrovic
-
Jankulovic, M., Poulsen, K., Brckalo, T., Bobic, S., Lindner, B., Petersen, A. (2008)
A novel
recombinantly produced banana lectin isoform is a valuable tool for glycoproteomics and a potent
modulator of the proliferation response in CD3+, CD4+, and CD8+ populations of human PBMCs.

International Journal of Biochemistry and Cell Biology 40(5), 929
-
41. (
RG2
)


Dimitrijevic, R., Stojanovic, M., Zivkovic, I., Petersen, A., Jankov, R.M., Dimitrijevic, Lj., Gavrovic
-
Jankulovic, M.
(2009)
The identification of a low molecular mass bacteriocin rhamnosin A, produced by
Lactobacillus
rhamnosus

strain 68
. Journal of Applied Microbiology doi: 10.1111/j.1365
-
2672.2009.04539.x

(RG2)


Jovanovic, B.V., Dimitrijevic
-
Sreckovic, S.V., Mandic, M.Lj. (2008)
Serum N
-
acetyl
-

-
D
-
杬畣潳a浩湩摡se
灲潦楬es⁩渠瑹灥 1 摩d扥瑥t sec潮摡特⁣潭灬oca瑩潮猺 ca畳es 潦 c桡湧敳 a湤⁳楧湩晩ia湣e 潦
摥瑥牭t湡瑩潮t

Journal of Clinical Laboratory Analysis, 22, 307
-
313. (
RG5
)


Nikolic, M., Vranic, D., Spiric, A., Batas, V., Nikolic
-
Kokic, A., Radetic, P., Turubatovic, L., Blagojevic, D.P., Jones,
D.R., Niketic, V., Spasic, M.B. (2006)
Could cholesterol bound to haemoglobin be a missing link for the
occasional inverse relationship between superoxide dismutase and glutathione peroxidase activities?

Biochemical Biophysical Research Communication, 348(1), 265
-
270. (
RG2
)

Selected publications: Food
quality and safety


Polovic, N., Pjanovic, R., Burazer, L., Velickovic, S., Jankov, R., Cirkovic Velickovic, T. (2009)
Acid
-
formed
pectin gel delays major incomplete kiwi fruit allergen Act c 1 proteolysis in
in vitro

gastrointestinal
digestion
, Journal of the Science of Food and Agriculture 89(1), 8
-
14. (
RG1
)


Polovic
,
N
.,
Blanusa
,
M
.,
Gavrovic
-
Jankulovic
,
M
.,
Atanaskovic
-
Markovic
,
M
.,
Burazer
,
L
.,
Jankov
,
R
.,
Cirkovic
Velickovic
,
T
. (2007)
A matrix effect in pectin
-
rich fruits hampers digestion of allergen by pepsin in vivo
and in vitro
.
Clinical and Experimental Allergy,

37(5), 764
-
71.

(RG1)



Blanusa, M., Perovic, I., Popovic, M., Polovic, N., Burazer, L., Milovanovic, M., Gavrovic
-
Jankulovic, M., Jankov,
R., Cirkovic Velickovic, T. (2007)
Quantification of Art v 1 and Act c 1 being major allergens of mugwort
pollen and kiwi fruit extracts in mass
-
units by ion
-
exchange HPLC
-
UV method.

Journal of Chromatography,
B: Analytical Technologies in the Biomedical and Life Sciences, 857(2), 188
-
194. (
RG1
)


Gavrovic
-
Jankulovic, M., Spasic, M., Cirkovic Velickovic, T., Stojanovic, M., Inic
-
Kanada, A., Dimitrijevic, L.,
Lindner, B., Petersen, A., Becker, W.M., Jankov, R.M. (2008)
Quantification of the thaumatin
-
like kiwi allergen
by a monoclonal antibody
-
based ELISA.

Molecular Nutrition Food Research, 52(6), 701
-
7.

(RG2)


Popovic, M., Milovanovic, M., Burazar, L., Vuckovic, O., Hoffmann
-
Sommergruber, K., Knulst, A., Lindner, B.,
Petersen, A., Jankov, R., Gavrovic
-
Jankulovic, M. (accepted May 2009)
Cysteine proteinase inhibitor Act d 4 is
a functional allergen contributing to the clinical symptoms of kiwifruit allergy
. Molecular Nutrition Food
Research.
(RG2)


Vidovic, S., Stojanovic, B., Veljkovic, J., Prazic
-
Arsic, L., Roglic, G., Manojlovic, D. (2008)
Simultaneous
determination of some water
-
soluble vitamins and preservatives in multivitamin syrup by validated
stability
-
indicating high
-
performance liquid chromatography method
. Journal of Chromatography A, 1202(2),
155
-
162.
(RG6)


Stanic D., Monigioudi E. Dilek E., Radosavljevic J., Lantto R., Mattinnen M., Vuckovic O., Bucher J., Cirkovic
Velickovic T (2009)
Allergenicity and digestibility of cross
-
linked beta
-
casein
. Molecular Nutrition Food
Research, in press.
(RG1)

Selected publications: Molecular
Biotechnology and Enzymology


Milosavi
c,
N.,
Prodanovic, R., Jankov, R.M. (2007)
A simple and efficient one
-
step, regioselective, enzymatic
glucosylation of arbutin by a
-
glucosidase.

Tetrahedron Letters, 48, 7222
-
7224. (
RG3
)


Milosavi
c,
N.,
Prodanovic, R., Jovanovic, S., Vujcic, Z. (2007)
Immobilization of glucoamylase via its
carbohydrate moiety on macroporous poly(GMA
-
co
-
EGDMA).

Enzyme and Microbial Technology, 40, 1422
-
1426.
(RG3)


Milovanovic, A., Bozic, N., Vujcic, Z. (2007)
Cell wall invertase immobilization within calcium alginate beads.

Food Chemistry,

104(1), 81
-
86. (
RG4
)


Vujcic, Z., Dojnov, B., Milovanovic, A., Bozic, N. (2008)

Purification and Properties of the Major Leucyl
Aminopeptidase from
Solanum tuberosum
Tubers.

Fruit, Vegetable and Cereal Science and Biotechnology,
2(1), 125
-
130. (
RG4
)


Dojnov, B., Bozic, N., Nenadovic, V., Ivanovic, J., Vujcic, Z.

(2008)

Purification and properties of midgut α
-
amylase isolated from
Morimus funereus

(Coleoptera: Cerambycidae) larvae.
Comparative Biochemistry and
Physiology, 149B, 153
-
160. (
RG4
)


Bozic, N., Ivanovic, J., Nenadovic, V., Bergstrom, J., Larsson, T., Vujcic, Z. (2008)
Purification and properties of
major midgut leucyl aminopeptidase of
Morimus funereus

(Coleoptera, Cerambycidae) larvae.

Comparative
Biochemistry and Physiology 149B, 454

462. (
RG4
)


Momic, T., Vujcic, Z., Vasic, V. (2008)
Kinetics of Inhibition of Peroxidase Activity of Myeloperoxidase by
Quercetin.

International Journal of Chemical Kinetics, 40(7), 384
-
394. (
RG4
)


Radulovic, M., Cvetkovic, O., Nikolic, S., Djordjevic, D., Jakovljevic, D., Vrvic, M. (2008)
Simultaneous
production of pullulan and biosorption of metals by
Aureobasidium pullulans

strain CH
-
1 on peat
hydrolisate.

Bioresource Technology, 99
,
6673
-
6677. (
RG5
)


Ilic, S., Konstatinovic, S., Veljkovic, V., Savic, D., Lazic, M., Gojgic
-
Cvijovic, G. (2008)
Impact of
carboxymethylcellulose on morphology and antibiotic production by
Streptomyces hygroscopicus
.
Current Microbiology, 57,

8
-
11. (
RG5
)


Stanic D., Radosavljevic J., Polovic N., Kostic
-
Jadranin M., Cirkovic Velickovic T.,
Removal of N
-
terminal
peptide from beta
-
lactoglobulin by contaminants in phenol oxidase preparations
. (2009) International Dairy
Journal, in press (
RG1
)