WATERS Laboratory

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20 Φεβ 2013 (πριν από 4 χρόνια και 1 μήνα)

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CWRS Advisory Board Meeting

March 1, 2007

Gretchen Grover


Chemist

Christal Wade
-

Microbiologist

Rick Fowler


Environmental Biotechnologist





WATERS Employees


Director:
Jana Fattic


Lab Analysts:


Microbiology Department: Rick Fowler, Christal Wade


Chemistry Department: Gretchen Grover, Dong Li


Office Associate:
Kristy Mayes


Students:
Samantha Kramer, Rebekkah Lively,
Jennifer Dean


Customers



Private


Total Coliform/E. Coli, Metals, Ions, Atrazine


Wastewater


BOD, pH, TRC, DO, TSS, Fecal Coliform, Ammonia Nitrogen


Storm water


COD, Oil and Grease, TDS, TSS


Drinking water


Total Coliform/E.Coli


Oil Wells


pH, TDS, Specific Gravity, Chloride


Research Projects


TOC, Atrazine, Metals, Ions, DNA

Certifications


Drinking water Certification


January Audit


Inorganic Certification


June Audit


Cryptosporidium


Long Term 2 Enhanced
Surface Water Treatment Rule
(LT2)


Accept outside samples under
LT2 and provides field
filtration apparatus


www.epa.gov/safewater/disinfection/
LT2/lab_aprvlabs.html


Chemical Hygiene Plan


Chemical Hygiene Officer

Projects


Mammoth Cave


Flood Pulse Event


City of Bowling Green


River & Cave System monitoring


Watershed Watch


Upper Green River watershed monitoring (E.coli)


TMDL


Analyzing samples for this project


University Research


Support WKU faculty as well as other university projects



Quality Assurance


Continued revision and updating of SOP to
the 21
st

Edition Standard Methods


QA/QC Improvements


New Standards


PT/QC (quarterly)


USGS


APG


Important Purchases

Chemistry


Accumet excel XL60


TKN Digestor


Konelab


Microbiology


Bench top water bath


FastGel electrophoresis apparatus


PCR Workstation


Qubit fluorometer


Microcentrifuge

Where do we go from here?


Current Capabilities


Cryptosporidium


Konelab Analyses


Drinking water, Wastewater, Storm water analyses


Bacteriological/DNA analyses



Future Capabilities


Unregulated Contaminant Monitoring Rule (UCMR2)


Organics (Work toward a GC MS/MS and HPLC)


Radionuclides


Direct a Chemical Laboratory Certification Program for
Kentucky


Voluntary Wastewater Certification






Where do we go from here?


New customers bring more cash flow


11 new customers


Networking; customer surveys


Pick up service


Consultation services


Sample collection services

Microbiology
Programs

Christal Wade

Drinking Water Microbiology

Sample Type Analyzed
Number Analyzed/year
Drinking Water (Public)
2080
Drinking Water (Private)
25
Wastewater
0
Surface (raw)
745
Total
2850
Laboratory Sample Load
Completed latest On
-
Site Audit in January 2007

EPA Approval for Crypto


WATERS Lab received approval from EPA
for the analysis of Cryptosporidium on
October 18, 2006.


Currently have a cooperative agreement
with Appalachian States Analytical, L.L.C.


Total of 20 surface water clients


Will be starting monitoring in June



4 Contracts with other PWSs


From both Kentucky and West Virginia


Several quotes outstanding

Latest PT Results

From September 2006

Oocysts
Reported
Oocysts
Spiked
% Recovered
Cysts
Reported
Cysts
Spiked
%
Recovered
03604
152
195
78
100
201
50
03605
139
195
71
91
201
45
03606
138
180
77
0
0
N/A
75
48
5
7
RSD of % Recovery
EPA Protozoan Proficiency Testing Laboratory Results Summary
Lab ID: KY00002
Round: 0906
Filter: Envirochek HV
Sample ID
Cryptosporidium
Giardia
Mean % Recovery
Completed another set of PT samples in February 2007

Mean (%)
Median (%)
RSD (%)
Mean (%)
Median (%)
RSD (%)
Envirochek
1
35
35
31
41
41
21
Envirochek HV
45
45
43
42
46
48
27
Filta-Max
15
47
46
35
39
43
44
Filta-Max Xpress
4
29
27
34
29
26
41
Direct
Centrifugation
1
61
61
1
49
49
25
Continuous Flow
Centrifugation
1
69
68
5
62
62
5
CrypTest
1
59
65
23
5
5
141
All Versions
68
45
44
41
43
46
35
WATERS Lab
75
5
48
7
OPT Recovery Statistics for Each Method Variation
Giardia Recoveries
Cryptosprodium Recoveries
Method Version
# Labs
Cryptosporidium OPR
0.0
0.2
0.4
0.6
0.8
1.0
06247-02
06251-02
06264-02
06278-02
06292-02
06306-02
06320-02
06334-02
06348-02
06362-02
07011-02
07025-02
07038-02
Sample Identification
Percent Recovery
Ongoing Precision and Recovery

Mean Recovery 52.4%

Giardia OPR
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
06247-02
06251-02
06264-02
06278-02
06292-02
06306-02
06320-02
06334-02
06348-02
06362-02
07011-02
07025-02
07038-02
Sample Identification
Percent Recovery
Ongoing Precision and Recovery

Mean Recovery 41.1%

Education & Outreach


2 Hour Crypto Presentation


Approved for Drinking Water Operator CEU


Presented at KRWA Training Sessions


Will Present at KWWOA Annual Conference


1 Hour
E. coli

Presentation


Approved for Waste Water Operator CEU


Presented at KRWA Training Sessions


Will Present for KWWOA


Environmental
Biotechnology

Rick

Fowler

DNA Analysis Services


DNA extraction from water, soil, membrane
filters, pure cultures, swabs, etc.


Yield of total DNA measured with Qubit fluorometer,
sensitivity 0.2 x 10
-
12
g


Quantitative Real
-
Time PCR


Determines concentration of DNA from specific
organisms in a mixture, sensitivity <1 ppt


DNA cloning and sequencing



Cloning permits segregation of individual species
comprising environmental microbial communities


DNA sequencing yields unique gene sequences for
identification of organisms

Bacteria

Parasites

Algae

Fungi

Amoebae

Zooplankton



Limestone

Ca
13
CO
3

Field work in Mammoth Cave

Examples of clients supported
by WATERS DNA services


Louisville Water Company


Ammonia Oxidizing Bacteria in distribution system


Acanthamoeba

in eyewash stations


KSU Aquaculture Research Center


DNA analysis of bacteria, algae, and fungi used to
evaluate conditions for optimal shrimp production


Fungi in some tanks identified by cloning and DNA
sequencing to assess potential risk or benefit


National Cave and Karst Research Institute


Develop a universal monitoring scheme applicable
to caves nationwide and catalog any new
microorganisms indigenous to cave climates

Examples, continued


Mammoth Cave National Park


Ongoing monitoring of bacterial communities for
better understanding of karst aquifers and as
indicators of environmental changes


Monitor
Chlorella
DNA in surface waters to assist
with protection of endangered mussells


Oregon Caves National Monument


Cloning and sequencing of bacterial and fungal
communities on
-

and off
-
tourist trails to assess
environmental impacts of tourism


Abundance of iron
-
sulfur bacteria indicate growth in
absence of light is derived from chemical energy

Scientific Outreach,
Presentations, and Workshops


National Speleological Society


2001, 2006


National Cave and Karst Research Institute


2001, 2005


Geological Society of America


2001, 2004


National Park Service advisory panels


2001, 2002, 2003, 2004, 2005, 2006


ISEIS


2006