University of Washington

blaredsnottyΤεχνίτη Νοημοσύνη και Ρομποτική

15 Νοε 2013 (πριν από 3 χρόνια και 8 μήνες)

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Dr. Mehmet Sarikaya


University of Washington


Molecular Biomimetics Approach to Materials Synthesis, Assembly and
Function Using Genetically Engineered Proteins


University of Missouri
-
Rolla Ceramic Engineering Seminar Series Speaker

Thursday April 29, 20
04


McNutt Hall, Room 204

3:30pm


Biography:

Dr. Sarikaya earned his BS in Metallurgical Engineering from the Middle East Technical University in
Turkey in 1977. Dr. Sarikaya received his MS and PhD in Materials Science and Engineering from the
University

of California, Berkeley in 1979 and 1982. He did his post doctoral work at the Max
-
Planck Institute
fur Metallforschung in Germany and the Edgar Bain Fundamental Research Laboratories with U.S. Steel in
Pittsburgh PA. He was a lecturer at the Lawrence B
erkeley Laboratory for one year before he became an
assistant professor at the University of Washington in Seattle. Dr. Sarikaya has also been a visiting professor at
both Princeton University and Nagoya University. In 2001, he returned to the University

of Washington as an
Adjunct Professor of Chemical Engineering. He is currently a professor at the University of Washington. Dr.
Sarikaya is a member of many professional societies including; ACerS, MRS, American Chemical Society,
Microscopy Society of A
merica, American Society of Materials, and the American Physical Society. He also is
a reviewer to many journal publications and panels for proposals to NSF, NIH, DOE, ARO, DARPA, and
AFOSR. Dr. Sarikaya has had over 25 publications since 1999 and has ed
ited many books in the same period.


Abstract:

Introduction:
Physical and chemical functions of organisms are carried out by a very large number
(billions) of proteins, of different variety (~10
5

in humans), through predictable and self
-
sustaining interact
ions, that
are developed through evolution.
Purpose:

Using biology as a guide, our multifaceted objective is to select, design,
genetically tailor, synthesize, and utilize short polypeptides for potential molecular linkers in self
-
assembly, ordered
organiz
ation, and biofabrication of nanoinorganic materials and molecularly hybrid systems in nanotechnology
(molecular electronics, magnetics, and photonics) and nanobiotechnology (bio
-
sensors,
-
assays, and
-
materials).
Methods:

Using display techniques, we: i.
Select short polypeptide (7 to 14
-
amino acid) sequences that bind to
inorganic materials; ii. Use various microscopy (e.g., fluorescence microscopy and atomic force microscopy) and
spectroscopy (surface plasmon resonance) techniques to asses molecular bind
ing and assembly characteristics and
perform cross
-
specificity of the binders; iii. Tailor molecular structure and binding properties utilizing protein
modeling and genetic engineering (such as site directed mutagenesis), and finally iv. Explore the utilit
y of the
selected sequences for synthesis, assembly and growth of inorganic materials.
Results
: We adapted combinatorial
biology protocols (cell
-
surface and phage display) for the selection inorganic
-
binding polypeptides with high
affinity to specific mate
rials, e.g., noble metals (Pt and Pd), oxides (SiO
2
, Al
2
O
3
, ZnO, Cu
2
O), and semiconductors
(GaN). We used these inorganic
-
binding polypeptides in materials synthesis and morphogenesis; assembled them in
self
-
organized patterns, used them as molecular erect
ors for immobilization of target molecules (DNAs, proteins,
phage and cells); and hybridized them with functional synthetic molecules for utility in nanoelectronics and
photonics.
Conclusions:

The success of engineered polypeptides in molecular biomimetics

is based on i. Molecular
recognition, ii. Self
-
assembly, and iii. Genetic tailoring of structures and functions.

Supported by the USA
-
ARO through the DURINT program under Grant No. DAAD19
-
01
-
1
-
0499.


A meeting of the UMR Chapter of the American Ceramic
Society and the Missouri Chapter of Keramos will follow the Seminar in
McNutt 211.

Contact Information:

Ceramic Engineering Department: (573) 341
-
4401

Seminar Selection Committee: Natalie Vanderspiegel nnvander@umr.edu, Jim Zimmermann jwztyf@umr.edu