Horizontal Transfer

beefzoologistΒιοτεχνολογία

21 Φεβ 2013 (πριν από 4 χρόνια και 10 μήνες)

120 εμφανίσεις

Molecular Microbiology

Genetic Recombination


Vertical


Binary fission


Budding


Horizontal


Conjugation


Transduction


Transformation

Conjugation

Transduction

Transformation

Griffith Experiment

Biotechnology


Recombinant DNA


Genetic Engineering


Disease treatments


SCID


SSA


CF


Make products


Vaccines


Enzymes


Insulin


Hormones

Plasmids


DNA


Replicate
independently


Genetic Vectors


Small


Stable


Marker inserted


Gene expression

Background


Donor


Recipient


Competent Cell


Natural


Change cell wall


Change PM


Artificial


Temperature


Salts (CaCl2)


Marker


Cloning Site

LB Broth and Agar


Lysogeny Broth


Luria Broth


Luria
-
Bertani


Nutritionally Rich


Peptides, casein


Vitamins


Minerals (NaCl)


Trace elements


Biotech


Cultivate E.coli

Procedure


Follow lab manual!!


Reader


Directs


Keeps time


Materials


LB plates


2 types


LB


LB
-
amp


2 situations


Before


After


Label, Label, Label


pGreen plasmid


CaCl2


Micropipets


Spreader


Loop inoculation


Temperature


Incubator


Baths


Ice


Heat


Results


Marker


Fluorescence


Cloning site


R
-
ampicillin


Colony observation


Natural light


UV light


Calculate
transformation
efficiency

Questions?

Glow Fish